LED 광질이 차나무 기내배양묘의 생육 및 광합성에 미치는 영향
Effect of Light-emitting Diodes on Photosynthesis and Growth of in vitro Propagation in Tea Tree
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
차나무의 기내배양 과정 중 증식배양 단계에서 LED 광질 조건에 따른 기내배양묘의 생육 특성과 광합성에 미치는 영향을 조사하였다. 광질은 적색광(R), 청색광(B), 혼합광(R+B+W)을 사용하여 처리하였고, 형광등(F)을 대조구로 하였다. 초장 생육은 적색광에서 가장 좋았으며, 특히 뿌리 생육에 있어서 혼합광은 길이와 표면적 증대에 효과적인 것으로 나타났다. 또한 T/R율, 엽록소 함량은 혼합광 처리에서증가하는 것으로 나타났다. 엽록소 형광반응 이미지 촬영 결과 모든 처리구에서 광질에 따른 Fv/Fm의값은 현저한 차이가 없었다. 그러나 배양묘의 NPQ는 청색광 처리에서 가장 많이 증가하여, 다른 광질과 유의적인 차이를 보였으며, 광합성 효율을 억제시키는 것으로 나타났다. 이상의 결과로 차나무 기내배양은 배양목적에 따라 광질을 선택하는 것은 매우 중요하며, 차나무 기내배양 시 건실한 식물 생산을위해서는 혼합광 처리가 유리할 것으로 판단된다. 본 연구결과는 차나무 대량증식 및 우량묘 생산 등에기초자료로 활용할 수 있을 것으로 판단된다.
Abstract
The influences of light generated by LEDs on shoot growth and photosynthesis of Tea plant(Camellia sinensis L.) were evaluated. The growth characteristics were investigated after 45 days of culture under four different light qualities: fluorescent lamp, red LED, blue LED, red+blue+white LED. Shoot growth was promoted by red light, especially root length and area were further promoted under the red+blue+white LED. Also, T/R ratio and Chlorophyll content were highest in red+blue+white. Fluor Cam was used to measure the fluorescence images of the plants, inhibition of photochemical efficiency(Fv/Fm) were not changed in all treatment. However, non-photochemical quenching(NPQ) were found rapidly increasing in blue LED, these results were that blue LED were inhibit photosynthetic efficiency and must be considered for efficiently in vitro cultivation of the tea plant. The above results suggest that light qualities could be an important factor to foster in vitro growth of the species. Also, In order to produce healthy plants, it is effective to using light qualities of red+blue+white LED on in vitro culture of the tea plant. These results could be used to mass propagating shoot and produce of healthy seedling.
참고문헌 (37)
- Chung DH, "Components and efficacy of tea", Ph D, pp.2004
- Debergh P, "Reconsideration of the term vitrification as used micropropagation", Pl Cell Tissue Organ Cult, 30, pp.135~140 (1992)
- Demmig B, "Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants", Planta, 171, pp.171~184 (1987)
- Fankhause C, "Light control of plant development", Annu. Res. Cell Dev. Biol, 13, pp.203~229 (1997)
- Haase D, "Morphological and physiological evaluation of seedling quality", USDA Forest Service Proceedings (2007)
- Heo JW, "Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provides by a light-emitting diode (LED)", Plant Growth Regul, 38, pp.225~230 (2002)
- Heo JW, "Influence of light quality and photoperiod on flowering of cyclamen ersicum Mill. cv ‘Dixie White’", Plant Growth Biol, 40, pp.7~10 (2003)
- 황정규, "차광재배에 의한 찻잎 성분 및 말차 품질 변화", 한국차학회지, 24(2), pp.40~49 (2018), 한국차학회[DOI]
- James AR, "Factors affecting CO2 assimilation, leaf injury and growth in salt-stressed durum whear", Functional Plant Biology, 29(12), pp.1393~1403 (2002)
- Jeong SC, "Development of Mass Propagation Techniques of Camellia sinensis through In vitro Culture", The Plant Res. Soc. of Kor, 13(1), pp.69~71 (2000)
- Kautsky H, "Neue Versuche zur Kohlens aureassimilation", Naturwissenschaften, 19(48), pp.964~ (1931)
- 김길자, "pH, 무기염류, 생장조절제 및 광 조건이 차나무의 기내 배양에 미치는 영향", 한국차학회지, 17(3), pp.30~35 (2011), 한국차학회
- 김기선, "한일 말차의 성분 비교", 한국식생활문화학회지, 19(2), pp.177~183 (2004), 한국식생활문화학회
- Kim SJ, "Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro", Sci. Hort, 101, pp.143~151 (2004)
- Kim YG, "Propagation of Camellia sinensis (L.)O. Kuntze by Tissue Culture and Physiological Activities of Methanol Extracts", pp.2012
- Kurilcik A, "In vitro culture of chrysanthemum plantlets using light-emitting diodes", Central Euro J. Biol, 3(2), pp.161~167 (2008)
- Effect of light intensity on chlorophyll contents in the leaves of several species of conifers and hardwoods subjected to artificial shading treatment, "Kwon KW", ~ (1996)
- 권민경, "LED 파장이 거베라 기내배양묘의 생육에 미치는 영향", 화훼연구, 20(4), pp.233~237 (2012), 한국화훼학회[DOI]
- 이종원, "건강기능식품의 시장현황 및 인삼시장의 전망", Journal of Ginseng Research, 29(4), pp.206~214 (2005), 고려인삼학회
- O. Kuntze) using Cold Tolerance Gene, , Kyungpook National Univ, 2007
- Lurie S, "Determining chilling injury induction in green peppers using nondestructive pulse amplitude modulated(PAM)fluorometry", J. of American Soc. for Horti. Sci, 119, pp.59~62 (1994)
- Masojidek J, "Change in chlorophyll fluorescence quenching and pigment composition in the green Chlorococcum sp. Grown under nitrogen dificiency and salinity stress", J. of Applied Phycology, 12, pp.417~426 (2000)
- 문흥규, "희귀 및 멸종위기 식물 덩굴용담의 기내생장에 미치는 광질 및 환기효과", Journal of Plant Biotechnology, 35(3), pp.215~221 (2008), 한국식물생명공학회
- Moreira da Silva MH, "The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer", Plant Cell Tissue Organ Culture, 51, pp.187~193 (1997)
- Morrow RC, "LED lighting in horticulture", Hort.Sci, 43, pp.1947~1950 (2008)
- Okamoto K, "Development of light quality on some greenhouse crops", Sci. Hort, 33, pp.27~36 (1994)
- Okamoto K, "Development of plant growth apparatus using blue and red LED as artificial light source", Acta Hort, 440, pp.111~116 (1996)
- 박장현, "가루차용 차엽의 생육 및 성분", 한국약용작물학회지, 10(5), pp.379~383 (2002), 한국약용작물학회
- Poudel PR, "Effect of red and blue-light-emitting diodes on growth and morphogenesis of grapes", Plant Cell Tiss. Org. Cult, 92, pp.147~153 (2008)
- Rogers HH, "Root to Shoot Ratio of Crops as Influenced by CO2", Plant Soil, 187, pp.229~248 (1996)
- Sakurai M, "Induction of anthocyanin accumulation in rose suspension cultured cells by conditioned medium of strawberry suspension cultures", Plant Cell Tissue Organ Cult, 50, pp.211~214 (1997)
- Shin KS, "Induction of betalain pigmentation in hairy root of pigmentation in hairy roots of red beet under different radiation sources", Biol. Plant, 47, pp.149~152 (2003)
- Shin KS, "The effect of light quality on the gorwth and development of in vitro cultured Doritaenopsis plants", Acta Physiolol. Plant, 30, pp.339~343 (2008)
- 손윤자, "기내배양에 의한 차나무 대량증식기술개발", 한국차학회지, 13(1), pp.79~92 (2007), 한국차학회
- 성주한, "염화칼슘 처리가 산벚나무 엽의 엽록소형광반응과 광합성기구에 미치는 영향", 한국산림과학회지, 99(6), pp.922~928 (2010), 한국산림과학회
- Tanaka M, "The use of light-emitting diodes (LED) as a novel light source for micropropagation", Proc. of The Korean-Japan Joint Symp. on Transplant Production in Horticultural Plants (1999), Chungbuk National. Univ
- 유성녕, "고추의 엽록소 형광 이미지 분석법에 의한 한발스트레스 지표화 가능성", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 45(5), pp.676~682 (2012), 한국토양비료학회[DOI]