희귀 수종 미선나무(Abeliophyllum distichum Nakai.)의 기내 증식 및 발근에 미치는 LED (light emitting diode) 효과
Effect of LEDs on shoot multiplication and rooting of rare plant Abeliophyllum distichum Nakai
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초록
This study was conducted to elucidate the effectof light sources and explant types on in vitro shoot multiplicationand rooting of a rare and endangered plantAbeliophyllum distichum. Both apical buds and axillary budswere used as explants under 4 different light sources, coolwhite florescent light (F), 100% blue light-emitting diode(LED) (B), 50% blue and 50% red LED mixture (BR), and100% red LED (R). Clear difference was observed in termsof shoot proliferation by light sources types but not byposition-dependent explant types. Multiple shoot inductionrates were enhanced under both B and BR light sources. Spontaneous rooting was induced in shoot induction mediumunder B light source. Both the rates of rooting and numbersof roots per explant were higher in apical bud explantscompared to axillary bud explants. Interestingly R lightsource stimulated shoot elongation but inhibited root development. Therefore, our results suggest that the use of apicalbud explants under B or BR light sources is suitable for invitro micropropagation of a rare and endangered plantspecies, Abeliophyllum distichum.
Abstract
This study was conducted to elucidate the effectof light sources and explant types on in vitro shoot multiplicationand rooting of a rare and endangered plantAbeliophyllum distichum. Both apical buds and axillary budswere used as explants under 4 different light sources, coolwhite florescent light (F), 100% blue light-emitting diode(LED) (B), 50% blue and 50% red LED mixture (BR), and100% red LED (R). Clear difference was observed in termsof shoot proliferation by light sources types but not byposition-dependent explant types. Multiple shoot inductionrates were enhanced under both B and BR light sources. Spontaneous rooting was induced in shoot induction mediumunder B light source. Both the rates of rooting and numbersof roots per explant were higher in apical bud explantscompared to axillary bud explants. Interestingly R lightsource stimulated shoot elongation but inhibited root development. Therefore, our results suggest that the use of apicalbud explants under B or BR light sources is suitable for invitro micropropagation of a rare and endangered plantspecies, Abeliophyllum distichum.
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