광물질 첨가제가 한우 번식우 바닥의 화학적 성분, 미생물 성상 및 가스 발생량에 미치는 영향
Effects of Mineral Additives on Chemical Compositions, Microbes and Gas Emissions of Hanwoo Cow Manure
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초록
본 연구는 미네랄 첨가제가 한우사 바닥에 미치는 영향을 조사하였다. 실험은 경남 진주시 소재 한우 번식우 농장에서 실시하였다. 우방당미네랄 첨가제를 5 ㎏ 첨가하였으며, 시험구로는 무첨가구(CON); bentonite 시험구(BN); illite 시험구(IL); 및 fly-ash 시험구(FA)로 두었다. 각각의 시험구로 4개의 우방을 이용하였으며, 우방당 5마리 한우 암소를 공시하였다. 미네랄 첨가제를 처리한 후 각 우방당 5곳에서 0, 7, 14, 21 및 28일에 우분을 채취하였다. 샘플은 건물, 발효특성, 미생물 성상 및 가스 발생량 분석에 이용되었다. 전 기간 동안 건물과 acetate 함량은미네랄 첨가제에 의한 영향은 나타나지 않았다. 무첨가구에서 7, 14 및 28일에 유기물 함량은 가장 높았고(p<0.05), 조회분 함량은 가장 낮았다(p<0.05). IL 시험구에서는 14, 21 및 28일에 총 질소 함량이 가장 높았고(p<0.05), IL, BN 및 FA 시험구에서는 각각 14, 21 및 28일에 무첨가구보다대장균이 낮았다(p<0.05). FA 시험구에서는 28일에 살모넬라 수가 가장 높았다(p<0.05). IL 시험구에서는 28일에 암모니아 발생량이 가장 높았고(p<0.05), 황화수소는 전기간 동안 검출되지 않았다. 28일간 결과값을 평균하였을 때, 무첨가구에서 유기물 함량이 가장 높았고(p<0.05), 조회분함량이 가장 낮았다(p<0.05). IL 시험구에서 총 질소 함량이 가장 높았고(p<0.05), BN 시험구는 ㏗가 가장 높았다(p<0.05). 대장균 수는 IL 시험구에서 가장 낮았고(p<0.05), 살모넬라 수는 무첨가구 보다 IL과 FA 시험구에서 더 낮았다(p<0.05). 따라서, 본 연구에서 광물질 첨가제는분 중 가스 발생량에는 영향을 미치지 않았으나, 병원성 미생물인 대장균은 illite와 fly-ash 처리 시에 억제되는 효과가 있었다.
Abstract
The present study investigated effects of mineral additives on Hanwoo manure in barns. The present study was conducted in Hanwoo cow Farm. The manure in barns was applied with mineral additives at 5 ㎏/barn following: without additives (CON); bentonite (BN); illite (IL); and fly-ash (FA). Each treatment used 4 barns as replication and each barn contained 5 cows. After mineral additives were spread, the manure in barns was sub-sampled from 5 sides of pen at 0, 7, 14, 21 and 28 days. The sub-samples were used for analyses of chemical compositions, fermentation characteristics, microbial counts and gas emissions. The concentrations of dry matter (DM) and acetic acid were not affected by mineral additives on all days. CON barn had highest concentrations of organic matter (p<0.05) but lowest concentrations of crude ash (p<0.05) at 7, 14 and 28 days. IL barns had highest concentrations of total N (p<0.05) at 14, 21 and 28 days. IL, BN and FA barns had lowest Escherichia coli (E. coli) than CON barn (p<0.05) at 14, 21 and 28 days, respectively. FA barn had highest Salmonella (p<0.05) at 28 day. IL barns had highest concentrations of ammonia-N gas (p<0.05) at 28 day. On other hand, hydrogen sulfide gas was not detected on all daysz. In the mean of results for 28 days, CON barn had highest concentrations of organic matter (p<0.05) but lowest concentrations of crude ash (p<0.05). IL barn had highest concentrations of total N (p<0.05) and BN barn had highest ㏗ (p<0.05). E. coli was lowest (p<0.05) in IL barn and Salmonella were lower (p<0.05) IL and FA barns than CON barn. In the present study, the mineral additives did not affect the gas production of cow manure, but the pathogenic microbes such as E. coli were inhibited by illite and fly-ash.
참고문헌 (40)
- Angelidaki I, "Effects of free long-chain fatty acids on thermophilic anaerobic digestion", Appl. Microbiol. Biotechnol, 37, pp.808~812 (1992)
- AOAC, "Official methods of analysis" (1990), Association of Official Analytical Chemists
- Rafael Borja, "Influence of ammonia concentration on thermophilic anaerobic digestion of cattle manure in upflow anaerobic sludge blanket (UASB) reactors", Process Biochemistry, 31(5), pp.477~483 (1996), Elsevier BV[DOI]
- Fiona P. Brennan, "Clay mineral type effect on bacterial enteropathogen survival in soil", Science of The Total Environment, 468-469, pp.302~305 (2014), Elsevier BV[DOI]
- Diane Burgess, "Do People Value The Welfare Of Farm Animals?. Les gens s'interessent-ils au bien-etre animal?. Messen die Menschen dem Wohl von Nutztieren einen Wert bei?", EuroChoices, 4(3), pp.36~43 (2005), Wiley[DOI]
- Chungeng W, "Evaluation of zeolites synthesized from fly ash as potential adsorbents for wastewater containing heavy metals", J. Environ. Sci, 21, pp.127~136 (2009)[DOI]
- A Fuentes, "Phytotoxicity and heavy metals speciation of stabilised sewage sludges", Journal of Hazardous Materials, 108(3), pp.161~169 (2004), Elsevier BV[DOI]
- F. Gottardo, "Effect of the manger space on welfare and meat quality of beef cattle", Livestock Production Science, 89(2-3), pp.277~285 (2004), Elsevier BV[DOI]
- Jajere SM, "A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance", Vet. World, 12, pp.504~521 (2019)[DOI]
- 전경호, "수세탈취시스템을 이용한 축산악취저감에 관한 연구", 축산시설환경학회지, 16(1), pp.21~28 (2010), (사)한국축산환경학회
- Kang MI, "Survey on Korean-native calves diseases and mortality", Korean J. Vet. Serv, 24, pp.223~241 (2001)
- Kim DH, "A survey on current state of odor emission and control from livestock operations", J. Anim. Environ. Sci, 19, pp.123~132 (2013)
- 김동혁, "환경유래 젖소유방염 저감을 위한 우분뇨 탈수 시스템의 탈수 및 유방염 원인체 제균 효과 규명", 대한수의학회지, 49(3), pp.257~263 (2009), 대한수의학회
- Kim DK, "Effect of pen floor condition depending upon housing orientation on the performance of finishing Hanwoo steers", J. Lives. Hous. & Env, 10, pp.37~46 (2004)
- 김삼철, "축분 첨가제를 한우분에 첨가시 pH와 유해성 병원균에 미치는영향에 관한 연구", 한국환경과학회지, 21(12), pp.1529~1533 (2012), 한국환경과학회[DOI]
- John C. Kissel, "Potential emissions of volatile and odorous organic compounds from municipal solid waste composting facilities", Biomass and Bioenergy, 3(3-4), pp.181~194 (1992), Elsevier BV[DOI]
- H. Leclerc, "Advances in the Bacteriology of the Coliform Group: Their Suitability as Markers of Microbial Water Safety", Annual Review of Microbiology, 55(1), pp.201~234 (2001), Annual Reviews[DOI]
- 이용복, "개방한우사와 개방육계사에서의 암모니아 발생량 산정", 농업생명과학연구, 47(6), pp.193~201 (2013), 농업생명과학연구원[DOI]
- Ronghua Li, "Nutrient transformations during composting of pig manure with bentonite", Bioresource Technology, 121, pp.362~368 (2012), Elsevier BV[DOI]
- Y Mercier, "Lipid and protein oxidation in vitro, and antioxidant potential in meat from Charolais cows finished on pasture or mixed diet", Meat Science, 66(2), pp.467~473 (2004), Elsevier BV[DOI]
- Roxane Milleret, "Root, mycorrhiza and earthworm interactions: their effects on soil structuring processes, plant and soil nutrient concentration and plant biomass", Plant and Soil, 316(1-2), pp.1~12 (2009), Springer Science and Business Media LLC[DOI]
- "Results of collection civil complaints from environmental departments of local governments" (2019)[URL]
- Muck RE, "Storage temperature effects on proteolysis in alfalfa silage", Trans. ASAE, 31, pp.1005~1009 (1988)
- Obaje SO, "Clays and their industrial applications: Synoptic review", Int. J. Sci. Technol, 3, pp.264~270 (2013)
- Leilei Pan, "A new intelligent electronic nose system for measuring and analysing livestock and poultry farm odours", Environmental Monitoring and Assessment, 135(1-3), pp.399~408 (2007), Springer Science and Business Media LLC[DOI]
- Park HS, "Selection of microorganisms for probiotics and their characterization", J. Korean Soc. Food Sci. Nutr, 6, pp.433~440 (1998)
- J.W. Paul, "Relationship between volatile fatty acids, total ammonia, and pH in manure slurries", Biological Wastes, 29(4), pp.313~318 (1989), Elsevier BV[DOI]
- Phillips TD, "Characterization of clay-based enterosorvents for the prevention of aflatoxicosis", Adv. Exp. Med. Biol, 504, pp.157~171 (2002)[DOI]
- C. Pisa, "Effects of incorporation of vermiculite on carbon and nitrogen retention and concentration of other nutrients during composting of cattle manure", Bioresource Technology Reports, 9, pp.100383~ (2020), Elsevier BV[DOI]
- Roger PA, "Soil. Microbial. Technologies. Metting B", pp.417~455 (1993), Marcel Dekker
- STAT user’s guide: Version 9, "SAS", pp.2002, SAS Institute Inc
- Sheng J, "Effects of fly ash on the exchangeable heavy metals (Cu, Zn, Pb) during sewage sludge composting and land utilization", Huan. Jing. Ke. Xue, 28, pp.1367~1371 (2007)
- Song HJ, "Colibacillosis in domestic animals, a review", Korean J. Vet. Serv, 21, pp.413~429 (1998)
- Quan Wang, "Improving pig manure composting efficiency employing Ca-bentonite", Ecological Engineering, 87, pp.157~161 (2016), Elsevier BV[DOI]
- Ward WR, "Observational study of temperature, moisture, ㏗ and bacteria in straw bedding and faecal consistency, cleanliness and mastitis in cows in four dairy herds", Vet. Rec, 151, pp.199~206 (2002)[DOI]
- Jonathan W.C. Wong, "Reduction of indicator and pathogenic microorganisms in pig manure through fly ash and lime addition during alkaline stabilization", Journal of Hazardous Materials, 169(1-3), pp.882~889 (2009), Elsevier BV[DOI]
- 딩옌, "축사 내 황화수소와 암모니아의 저감방안 고찰: 한중비교", 한국산업보건학회지, 29(4), pp.442~451 (2019), 한국산업보건학회[DOI]
- Akio Yasuhara, "Identification of Odorous Compounds in Fresh and Rotten Swine Manure", Agricultural and Biological Chemistry, 48(12), pp.3001~3010 (2014), Oxford University Press (OUP)[DOI]
- 여민주, "우리나라 쇠고기 소비에 의한 생태발자국 추이와 예측", 환경영향평가, 25(4), pp.280~295 (2016), 한국환경영향평가학회[DOI]
- J.A. Zahn, "Correlation of Human Olfactory Responses to Airborne Concentrations of Malodorous Volatile Organic Compounds Emitted from Swine Effluent", Journal of Environmental Quality, 30(2), pp.624~634 (2001), Wiley[DOI]