Effects of Dietary n-3/n-6 Fatty Acid Ratio on In Vitro Fermentation Characteristics and Fatty Acid Profiles
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was conducted to examine the effect of dietary n-3/n-6 fatty acid (FA) ratio on in vitro dry matter digestibility (IVDMD), fermentation indices and FA profile. Rice bran was mixed with oil sources (cotton seed oil and linseed oil) to make the diets at 0.02, 0.29 and 0.61 of dietary n-3/n-6 FA ratio. These diets (0.5g) were placed into the incubation bottles with 40 ml of anaerobic culture medium, which contained rumen fluid and Van Soest medium at 1:2 ratio. Five replicates of each diet and two blanks were incubated at 39℃ for 48 hours. After incubation, the incubated contents were centrifuged. The residues were freeze-dried for DMD and FA analyses. The supernatant was used for pH, NH3-N and volatile fatty acid analyses. The concentrations of lactate (p<0.001) and iso-valerate (p<0.001) decreased linearly with increasing dietary n-3/n-6 FA ratio, but acetate concentration (p=0.056) and the ratio of acetate to propionate (p=0.005) was increased linearly. The concentrations of n-3, n-6 FA and the ratio of n-3/n-6 FA in residues increased (p<0.001) linearly with increasing dietary n-3/n-6 FA ratio, but C18:1n-9 FA concentration was decreased (p<0.001) linearly. With these results, it could affect fermentation characteristics and FA profile of rumen content by dietary n-3/n-6 FA ratio.
Abstract
This study was conducted to examine the effect of dietary n-3/n-6 fatty acid (FA) ratio on in vitro dry matter digestibility (IVDMD), fermentation indices and FA profile. Rice bran was mixed with oil sources (cotton seed oil and linseed oil) to make the diets at 0.02, 0.29 and 0.61 of dietary n-3/n-6 FA ratio. These diets (0.5g) were placed into the incubation bottles with 40 ml of anaerobic culture medium, which contained rumen fluid and Van Soest medium at 1:2 ratio. Five replicates of each diet and two blanks were incubated at 39℃ for 48 hours. After incubation, the incubated contents were centrifuged. The residues were freeze-dried for DMD and FA analyses. The supernatant was used for pH, NH3-N and volatile fatty acid analyses. The concentrations of lactate (p<0.001) and iso-valerate (p<0.001) decreased linearly with increasing dietary n-3/n-6 FA ratio, but acetate concentration (p=0.056) and the ratio of acetate to propionate (p=0.005) was increased linearly. The concentrations of n-3, n-6 FA and the ratio of n-3/n-6 FA in residues increased (p<0.001) linearly with increasing dietary n-3/n-6 FA ratio, but C18:1n-9 FA concentration was decreased (p<0.001) linearly. With these results, it could affect fermentation characteristics and FA profile of rumen content by dietary n-3/n-6 FA ratio.
참고문헌 (23)
- 김삼철, "A novel, wireless, automated system for measuring fermentation gas production kinetics of feeds and its application to feed characterization", ANIMAL FEED SCIENCE AND TECHNOLOGY, 123(124), pp.211~223, ELSEVIER SCIENCE BV
- Allen, K. G. D, "The role of n-3 fatty acids in gestation and parturition", Exp. Biol. Med, 226, pp.498~506 (2001)
- AOAC, "Official method of analysis" (1995), Association of Official Analytical Chemist
- Bilby, T. R, "Pregnancy, bovine somatotropin, and dietary n-3 fatty acids in lactating dairy cows: III. Fatty acid distribution", J. Dairy Sci, 89, pp.3386~3399 (2006)
- Chaney, A. L, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
- Chagas, L. M, "Invited Review: New perspectives on the roles of nutrition and metabolic priorities in the subfertility of high-producing dairy cows", J. Dairy Sci, 90, pp.4022~4032 (2007)
- Gulati, S. K, "Protection of conjugated linoleic acids from ruminal biohydrogenation and their incorporation into milk fat", Anim. Feed Sci. Technol, 86, pp.139~148 (2000)
- Jalč, D, "Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen", Czech J. Anim. Sci, 54, pp.229~237 (2009)
- Jenkins, T. C, "The biohydrogenation of linoleamide in vitro and its effects on linoleic acid concentration in duodenal contents of sheep", J. Anim. Sci, 80, pp.533~540 (2002)
- Jenkins, T. C, "Direct methylation procedure for converting fatty amides to fatty acid methyl ester in feed and digesta samples", J. Agric. Food Chem, 49, pp.2142~2145 (2001)
- Jouany, J. P, "Effect of rumen protozoa on nitrogen utilization by ruminants", J. Nutr, 126, pp.13335S~1346S (1996)
- Kim, S. C, "Effects of dietary n-6:n-3 fatty acid ratio on feed intake, digestibility, and fatty acid profiles of the ruminal contents, liver, and muscle of growing lambs", J. Anim. Sci, 85, pp.706~716 (2007)
- Mattos, R, "Effects of dietary fatty acids on reproduction in ruminants", Rev. Repro, 5, pp.38~45 (2000)
- Muck, R. E, "Storage temperature effects on proteolysis in alfalfa silage", Trans. ASAE, 31, pp.1005~1009 (1988)
- Pike, I. H, "The fats of life the role of fish", Lipid Technology, 13, pp.58~60 (2000)
- Raes, K, "Effect of fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review", Anim. Feed Sci. Technol, 113, pp.119~221 (2004)
- SAS, "Sas User’s guide. Statistics. Verson 8", SAS Institute, ~ (2002)
- Scollan, N. D, "Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle", Br. J. Nutr, 85, pp.115~124 (2001)
- Simopoulos, A. P, "Omega-3 fatty acids in health and disease and in growth and development", Am. J. Clin. Nutr, 54, pp.438~463 (1991)
- Simopoulos, A. P, "The importance of the omega-6", omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases, 2008, pp.233, 674~688
- Thatcher, W. W, "Strategies for improving fertility in the modern dairy cow", Theriogenology, 65, pp.30~44 (2006)
- Van Soest, P. J, "Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3568~3597 (1991)
- Wathes, D. C, "Polyunsaturated fatty acids in male and female reproduction", Biol. Reprod, 77, pp.190~201 (2007)