Antibacterial effects of Coptis chinensis Franch against Brucella abortus
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Brucellosis is an important bacterial zoonotic infection worldwide responsible for economic losses in livestock industries which represents a considerable and increasing public health burden. The disease is caused by Brucella which possess the ability to invade and replicate within phagocytes and may result to chronic infection, leading to difficulty in medical therapy of the disease. The treatment for brucellosis employs conventional principles that have been applied for a long time and a combination of antibiotics is currently used due to low efficacy of monotherapies. However, vital effects with respect to health and safety are neglected. Nevertheless, the preventive methods and treatments for brucellosis using traditional medicine have not yet been thoroughly studied, hence, alternative therapies such as the use of natural plant extracts as traditional medicine that are safe, efficient and economical should be explored in order to identify candidates that eliminate complications due to brucellosis. Coptis chinensis Franch (Huanglian) is a traditional Chinese medicinal herb and its extract has been known to possess strong antibacterial activity. In this study, the antibacterial effects of C. chinensis Franch extracts, and the major components of the herb namely berberine and palmatine, were investigated on B. abortus. The C. chinensis Franch ethanol extracts (CCFE) showed bactericidal effects at 1,000 μg/ml concentration and berberine at 100 μg/ml concentration. However, C. chinensis Franch and its components did not affect invasion and intracellular growth of B. abortus in RAW 264.7 cells. In conclusion, C. chinensis Franch ethanol extracts, water extract and its major components such as berberine and palmatine would be a beneficial antimicrobial agent without affecting phagocytic pathway within macrophages, and further study for the precise mechanisms of antibacterial effect would be necessary.
Abstract
Brucellosis is an important bacterial zoonotic infection worldwide responsible for economic losses in livestock industries which represents a considerable and increasing public health burden. The disease is caused by Brucella which possess the ability to invade and replicate within phagocytes and may result to chronic infection, leading to difficulty in medical therapy of the disease. The treatment for brucellosis employs conventional principles that have been applied for a long time and a combination of antibiotics is currently used due to low efficacy of monotherapies. However, vital effects with respect to health and safety are neglected. Nevertheless, the preventive methods and treatments for brucellosis using traditional medicine have not yet been thoroughly studied, hence, alternative therapies such as the use of natural plant extracts as traditional medicine that are safe, efficient and economical should be explored in order to identify candidates that eliminate complications due to brucellosis. Coptis chinensis Franch (Huanglian) is a traditional Chinese medicinal herb and its extract has been known to possess strong antibacterial activity. In this study, the antibacterial effects of C. chinensis Franch extracts, and the major components of the herb namely berberine and palmatine, were investigated on B. abortus. The C. chinensis Franch ethanol extracts (CCFE) showed bactericidal effects at 1,000 μg/ml concentration and berberine at 100 μg/ml concentration. However, C. chinensis Franch and its components did not affect invasion and intracellular growth of B. abortus in RAW 264.7 cells. In conclusion, C. chinensis Franch ethanol extracts, water extract and its major components such as berberine and palmatine would be a beneficial antimicrobial agent without affecting phagocytic pathway within macrophages, and further study for the precise mechanisms of antibacterial effect would be necessary.
참고문헌 (20)
- Acha, P., "Bacterioses and Mycoses", pp.28~45 (1980), Pan American Health Organization
- Al Dahouk, S., "Implications of laboratory diagnosis on brucellosis therapy", Expert Rev. Anti Infect. Ther, 9, pp.833~845 (2011)
- Anon, "WHO monograph on selected Medicinal Plants" (1999), World Health Organization
- Baldwin, C. L., "Macrophages and Brucella", Immunol. Ser, 60, pp.363~380 (1994)
- China Pharmacopoeia Committee, "Pharmacopoeia of the People’s Republic of China", pp.213~214 (2005), China Chemical Industry Press
- Corbel, M. J., "Brucellosis: an overview", Emerg. Infect. Dis, 3, pp.213~221 (1997)
- Detilleux, P. G., "Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro", Infect. Immun, 58, pp.2320~2328 (1990)
- Ding, DM., "Pharmacodynamic action & clinic of Chinese medical materials", pp.54~56 (1999), China Medicine technology Press
- Kim, S., "Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus Microb", Pathog, 37, pp.11~19 (2004)
- Kong, W. J., "Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry", J. Hazard. Mater, 168, pp.609~613 (2009)
- Liautard, J. P., "Interactions between professional phagocytes and Brucella spp", Microbiologia, 12, pp.197~206 (1996)
- Liu, L., "Analysis of four alkaloids of Coptis chinensis in rat plasma by high performance liquid chromatography with electrochemical detection", Anal. Chim. Acta, 737, pp.99~104 (2012)
- Magoffin, R. L., "The protection of intracellular brucella against streptomycin alone and in combination with other antibiotics", J. Lab. Clin. Med, 37, pp.924~930 (1951)
- Skalsky, K., "Treatment of human brucellosis: systematic review and meta-analysis of randomised controlled trials", BMJ, 336, pp.701~704 (2008)
- Solera, J., "Update on brucellosis: therapeutic challenges", Int. J. Antimicrob. Ag, 36(1), pp.S18~S20 (2010)
- Trujillo, I. Z., "Brucellosis", Infect. Dis. Clin. North Am, 8, pp.225~241 (1994)
- Ullah, R. W., "Genetic diversity, zoonotic risk and 'one health' initiative of bovine brucellosis", Res. J. Vet. Prac, 1, pp.5~9 (2013)
- Yang, F., "Application of analytical and preparative high-speed counter-current chromatography for separation of alkaloids from Coptis chinensis Franch", J. Chromatogr. A, 829, pp.137~141 (1998)
- Yin, L., "An economical method for preparative purification of five alkaloids from Coptis chinensis Franch by high-speed counter-current chromatography using singled prepared solvent system by GC", Am. J. Anal. Chem, 2, pp.411~421 (2011)
- Yuan, L., "Hypoglycemic and hypocholesterolemic effects of Coptis chinensis Franch inflorescence", Plant Foods Hum. Nutr, 61, pp.139~144 (2006)