비파괴성 측정기술을 이용한 한약자원식물의 종자특성평가
Characterization for Seeds of Korean Medicinal Plants Using Non-deductive Measurement Techniques
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초록
한약재는 전초, 잎, 뿌리, 종자, 열매 등 다양한 부위가 약용부위로 활용되고 있다. 우수한 한약재를안정적으로 생산하기 위해서는 종자의 특성 분석 및 발아율 검증을 통해 우량종자를 선발하고 발아율을향상시키는 것이 매우 중요 하다. 특히 한약자원식물의 종자는 다른 작물종자와 비교하면 모양과 크기및 내·외부 형태적 특징이 매우 다양하다. 본 연구에서는 비파괴측정기술인 광학현미경(Light microscopy)와 X-선 회절분석법(X-Ray Diffractometer)을 이용하여 한약자원식물의 종자의 내·외부 특성을 평가하여 건전종자를 파악하였고, 분광분석법(Hyperspectral system)을 이용하여 종자의 발아율을 예측 하였다. 23개의 한약자원식물 종자를 대상으로 외부형태(길이와 폭)와 내부형태를 관찰하여, 배(Embryo)의 상태가 건전한지 파악하였다. 종자표면이 거칠고 입체적인 표면을 가진 종자를 제외하고, 대부분의종자들은 건전성 여부가 파악되었다. 한약재 내복자(萊菔子, Raphanus seed)로 사용되는 무(Raphanussativus L.)종자를 선정하여, 192개의 종자를 임의적으로 선발한 후 국제종자검정협회(ISTA) 규정에 따라 발아율 실험을 실시하였다. 총 192립의 종자 중 150립의 종자가 발아하였고, 42립의 종자가 발아되지 않았으며, 이것을 바탕으로 발아 종자와 비발아 종자의 예측가능 모델 스펙트럼의 자료를 확보하여최적 모델개발에 적용하였다. 발아측정모델을 만들기 위해서 근적외선 분광분석기(FT-NIR), 단파 적외선 초분광기(SWIR) 및 가시/근적외선 초분광기(Vis/NIR)를 이용하여 발아 유무를 예측한 결과, 가시/근적외선 초분광기(Vis/NIR)를 이용한 분석 결과(Accuracy=90%)가 단파 적외선 초분광기(SWIR) 분석결과(Accuracy=71%) 및 근적외선 분광분석기(FT-NIR) 분석결과(Accuracy=85%)보다 예측율이 높았다. 본 결과를 바탕으로 한약자원식물 종자의 특성를 평가하고, 발아율을 예측하여 건전종자 및 우량종자를 선발하는 기술로 활용이 가능하리라 사료된다.
Abstract
Korean herbal medicines have been used for various medicinal parts such as whole plants,leaves, roots, seeds, fruits etc. In order to produce a good quality of products for Korean herbalmedicines, it is necessary to make conditions for superior selection and germination of seedsthrough the seed property and seed verification. Particularly, the seeds of Korean medicinalplants vary greatly in size and shape, and internal/external morphological features, unlikevegetable seeds. In this study, the non-destructive measurement technique using Light microscopy and X-ray diffractometer was evaluated the internal/external properties of the seeds determiningthe sound of the medicinal plant seeds in Korean herbal medicines but also using hyperspectralsystem was predicted for the germination rate. 23 different seeds of Korean medicinal plantswere examined for the status of internal and external length to target the seeds inside the stateof embryo weather a sound grasp. Except for the seeds with a rough and three-dimensionalcomplex surface, most of the seeds are whether health was identified. By selecting medicinalplant(Raphanus sativus L.) seeds used as a Korean herbal medicine, 192 randomly selectedseeds were carried out in accordance with the ISTA(International Seed Test Association) rulesfor seed germination test. From a total of 192 samples, 150 samples were sprouted and 42samples were not germinated. Based on these results, a total of 82 spectra germination has beenconfirmed germination and non-germination samples were applied to develop the optimummodel. To make the germination measurement model, SWIR(Short Wave Infrared Ray),Vis/NIR(Visible/ Near Infrared Ray), and FT-NIR(Fourier Transform - Near Infrared Ray) wereused to predict of isible germination. As the results, Vis/NIR predictive analysis(Accuracy=90%) had higher accuracy than SWIR predictive analysis(Accuracy= 71%) and FT-NIRpredictive analysis(Accuracy= 85%). Based on results in the seeds of Korean herbal medicinesusing non-deductive measurement techniques, it was provided prediction information aboutcharacteristics evaluation and germination for healthy and superior seeds.
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