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Effect of Homo or Heterofermentative Inoculants on Fermentation Characteristics and Aerobic Stability of Rye Silage

이성신|이현준|김동현|김삼철|곽연식|주영호|한옥규|Dimas H. V. Paradipta
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2017|농업생명과학연구 (Journal of Agriculture & Life Science)|제51권 제5호|pp. 81-89|DOI https://doi.org/10.14397/jals.2017.51.5.81

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참고문헌

키워드

Lactobacillus plantarumInoculantRye silageAerobic stabilityLactobacillus buchneri

초록

This study was conducted to estimate the effect of home or hetero fermentative lactic acidbacteria(LAB) on chemical composition, fermentation quality, and aerobic stability of rye silage. Rye forage was harvested at dough stage(28.9% of dry matter), chopped to 3-5 cm length, anddivided into 4 piles for different inoculations as treatment, following 1) No additives(CON); 2)Lactobacillus plantarum at rate of 1.5 x 105 cfu/g of fresh forage(LP); 3) L. buchneri at rate of1.2 x 105 cfu/g of fresh forage(LB); and 4) Mixture of LP and LB at 1:1 ratio(MIX). Ryesilage was ensiled into 20 L bucket silo in quadruplicate for 0, 1, 4, 7, and 100 day periods. After 100 days of ensiling, the silage treated with LB had lower acid detergent fibercontent(p<0.05), but higher in vitro dry matter digestibility(p<0.05). The LB and MIX reduced(p<0.05) pH more rapidly than CON and LP across the ensiling days, but had no differenceon 100 days. Silage treated LP had lowest(p<0.05) acetic acid, but highest(p<0.05) propionicacid. In contrast, LB treated silage had highest(p<0.05) acetic acid, but lowest(p<0.05)propionic acid with the absence of butyric acid. On microbial count, LP treated silage hadlowest(p<0.05) LAB, yeast, and aerobic stability, whereas LB and MIX treated silages hadhighest(p<0.05). Mold was not detected across all silages. Therefore, it could be concluded thatheterofermentative LAB solely or combo with homofermentative LAB might improve in vitrodry matter digestibility, fermentation characteristics, and aerobic stability of rye silage harvestedat dough stage.

Abstract

This study was conducted to estimate the effect of home or hetero fermentative lactic acidbacteria(LAB) on chemical composition, fermentation quality, and aerobic stability of rye silage. Rye forage was harvested at dough stage(28.9% of dry matter), chopped to 3-5 cm length, anddivided into 4 piles for different inoculations as treatment, following 1) No additives(CON); 2)Lactobacillus plantarum at rate of 1.5 x 105 cfu/g of fresh forage(LP); 3) L. buchneri at rate of1.2 x 105 cfu/g of fresh forage(LB); and 4) Mixture of LP and LB at 1:1 ratio(MIX). Ryesilage was ensiled into 20 L bucket silo in quadruplicate for 0, 1, 4, 7, and 100 day periods. After 100 days of ensiling, the silage treated with LB had lower acid detergent fibercontent(p<0.05), but higher in vitro dry matter digestibility(p<0.05). The LB and MIX reduced(p<0.05) pH more rapidly than CON and LP across the ensiling days, but had no differenceon 100 days. Silage treated LP had lowest(p<0.05) acetic acid, but highest(p<0.05) propionicacid. In contrast, LB treated silage had highest(p<0.05) acetic acid, but lowest(p<0.05)propionic acid with the absence of butyric acid. On microbial count, LP treated silage hadlowest(p<0.05) LAB, yeast, and aerobic stability, whereas LB and MIX treated silages hadhighest(p<0.05). Mold was not detected across all silages. Therefore, it could be concluded thatheterofermentative LAB solely or combo with homofermentative LAB might improve in vitrodry matter digestibility, fermentation characteristics, and aerobic stability of rye silage harvestedat dough stage.

참고문헌 (26)

  1. Adesogan AT, "The influence of treatment with dual-purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass", J. Dairy Sci, 87, pp.3407~3416 (2004)
  2. Adesogan AT., "Avoiding the two greatest silage problems", IProc. Proceedings 50th Florida Dairy Production Conference, pp.9~17 (2014)
  3. AOAC., "Official Method of Analysis", Association of Official Analytical Chemists, ~ (1990)
  4. Arriola KG, "Effect of applying bacterial inoculants containing different types of bacteria to corn silage on the performance of dairy cattle", J. Dairy Sci, 94, pp.3973~3979 (2011)
  5. Briggle LW, "Growing rye", USDA. Farmers’ Bull. No. 2146, ~ (1959)
  6. Chaney AL, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
  7. 최기춘, "신규 젖산균 첨가가 저 수분 호밀 사일리지의 발효 품질에 미치는 영향", 한국초지조사료학회지, 36(1), pp.23~28 (2016), 한국초지조사료학회
  8. Danner H, "Acetic acid increases stability of silage under aerobic conditions", Appl. Environ. Microbiol, 69, pp.562~567 (2003)
  9. Dewar WA, "The hydrolysis of grass hemicelluloses during ensilage", J. Sci. Food. Agric, 14, pp.411~417 (1963)
  10. 한옥규, "종자 생산량이 많은 호밀 신품종 “씨드그린”", 한국초지조사료학회지, 35(2), pp.105~111 (2015), 한국초지조사료학회
  11. Kang TW, "Effects of an esterase-producing inoculant on fermentation, aerobic stability, and neutral detergent fiber digestibility of corn silage", J. Dairy Sci, 92, pp.732~738 (2009)
  12. Kim DA, "Effects of sowing time and seeding rate on growth characteristics, winter survival and dry matter yield of forage rye", J. Kor. Soc. Grassl. Sci, 6, pp.164~168 (1986)
  13. Kim JG, "Effect of maturity at harvest and inoculants on the quality of round baled rye silage", J. Kor. Soc. Grassl. Sci, 19, pp.347~354 (1999)
  14. Kim JG, "Effects of maturity at harvest and wiliting days on quality of round baled rye silage", Asian Australas. J. Anim. Sci, 14, pp.1233~1237 (2001)
  15. 김종근, "국내 육성 사료작물의 생산성 및 사일리지 품질 평가", 한국초지조사료학회지, 35(2), pp.145~151 (2015), 한국초지조사료학회
  16. Kleinschmit DH, "The effects of various antifungal additives on the fermentation and aerobic stability of corn silage", J. Dairy Sci, 88, pp.2130~2139 (2005)
  17. Kung LJr, "The effect of treating alfalfa with lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows", J. Dairy Sci, 86, pp.336~343 (2003)
  18. McDonald P, "The biochemistry of silage" (1991), Chalcombe Publications
  19. Oliveira AS, "Meta-analysis of effects of homofermentative and facultative heterofermentative lactic acid bacteria inoculation on silage fermentation and aerobic stability and the performance of dairy cows", J. Dairy. Sci, 100, pp.4587~4603 (2017)
  20. Oude Elferink SJWH, "Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri", Appl. Environ. Microbiol, 67, pp.125~132 (2001)
  21. Parvin S, "Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and Rhodes grass silages", Anim. Feed Sci. Technolo, 160, pp.160~166 (2010)
  22. "SAS User’s Guide, Version 8", SAS Inst., Inc., ~ (2002)
  23. 송태화, "남부지역 논에서 사료맥류, 조생종 벼 및 하파귀리를 활용한 삼모작 작부체계 연구", 한국초지조사료학회지, 34(4), pp.227~233 (2014), 한국초지조사료학회
  24. Tilley JMA, "A two-stage technique for the in vitro digestion of forage crops", J. Br. Grassl. Soc, 18, pp.104~111 (1963)
  25. Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3583~3597 (1991)
  26. Weinberg ZG, "The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages", J. Appl. Bacteriol, 75, pp.512~518 (1993)

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