DNA 메틸화 억제제 처리방법에 따른 밀 염색체 및 발아 특성 비교
Comparison of Wheat Chromosomes and Germination Characteristics according to DNA Methylation Inhibitor Treatment Methods
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이 연구에서는 DNA 메틸화 억제제의 처리방법에 따라 밀 염색체와 발아 초기 생육 특성에 미치는 영향을 조사하였다. 종자를 DNA 메틸화억제제 수용액에 침종한 처리구와 증류수 침종 후에 발아 시 DNA 메틸화 억제제 수용액을 뿌리가 흡수하는 처리구의 체세포 분열 중기 염색체관찰 및 발아 초기의 생육을 조사하였다. 두 처리방법에서 초엽과 유근의 신장이 대조구보다 억제되는 것을 확인하였으며, 이러한 현상은 뿌리분열 조직 세포의 활동성에 DNA 메틸화 억제제가 영향을 주어 체세포 분열 지수가 낮아지는 것을 확인하였다. 초엽과 유근의 신장은 DNA 메틸화 억제제 수용액의 흡수처리구에서 침종처리구보다 유의미하게 더 억제되었지만 침종과 흡수처리구 간의 체세포 분열지수는 유의한 차리를보이지 않았다. 두 처리구(침종 및 흡수)에서 틈과 염색체 절단 같은 염색체 이상이 확인되었으며, 침종처리구에서는 염색체 풀림 현상과 짧은염색체가 추가로 발생하였다.
Abstract
This study is to identify the effect of different treatment methods of DNA methylation inhibitor on wheat chromosomes and germination characteristics. Coleoptile and radicle length was measured to observe germination characteristics according to the two treatment methods as seed imbibition and root absorption with DNA methylation inhibitor. Also, wheat mitotic metaphase chromosomes were observed, and mitotic index was measured to observe mitotic cell activity according to the treatment methods. Coleoptile and radicle was restricted on elongation and growth because mitotic indexes in imbibition and absorption treatment were lower than that in control. It can be explained activity of root tip meristem cell was damaged by DNA methylation inhibitor. The elongation and growth of coleoptile and radicle in the absorption were significantly worse than that in imbibition because roots directly absorbed DNA methylation inhibitor solution in absorption. However, mitotic indexes between two treatment methods were not significantly different. In mitotic metaphase under the imbibition, gap on wheat chromosomes were occurred like the absorption. Additionally, release of chromosomes and short chromosome were occurred only in the imbibition.
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