동백나무 종자의 Camelliaside B 분석을 위한 HPLC-DAD 정량 분석법 개발 및 평가
Validation of HPLC-DAD Method for Quantitative Analysis of Camelliaside B in Camellia japonica Seed Extract
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구는 동백나무 종자에 함유된 생리활성물질인 camelliaside B의 표준정량화를 위해 고성능 액체 크로마토그래피 (HPLC-DAD) 분석법을확립하고자 실시하였다. 동백나무 종자에 함유된 주요화합물은 MS/MS 분석, UV 흡광도, 화합물의 유지시간 등을 통해 얻은 결과에 바탕을두어 camelliaside B를 동백나무 종자 추출물의 지표물질로 선정하였다. Camelliaside B의 HPLC 분석법에 대한 특이성, 정확성, 정밀도 및정량한계 등을 검증하였다. Camelliaside B 분석을 위해 표준화된 HPLC 분석법은 0.99% 이상의 상관계수(R2)로 높은 선형성을 가지는 것을보여주었다. Camelliaside B의 회수율은 100.42-108.00%였으며, 검출한계 (LOD)와 정량한계 (LOQ)는 각각 0.084㎍/㎖, 0.254㎍/㎖였다. 동백나무 종자 지표물질 (camelliaside B)의 일중 정밀도는 각각 0.09-0.16 RSD였으며, 일간정밀도는 0.123 RSD였다. 표준화된 분석법으로 지역별로수집한 동백나무 종자의 camelliaside B의 함량은 0.279-2.05㎎/g 으로 다양하였다. HPLC 분석법으로 확인한 모든 매개변수는 기능성 원료인정을 위한 제출자료 작성 기준을 충족하였다. 따라서 본 연구에서 확립한 HPLC 분석법은 동백나무 종자 추출물 유래 건강 관련 기능성 제품또는 의약품 개발의 첫 단계를 제공할 수 있을 것이다.
Abstract
In this study, we attempted to establish a High-Performance Liquid Chromatography (HPLC) analysis method with Diode Array Detector (DAD) for standard quantification of camelliaside B, which are health functional food ingredient in seed of Camellia japonica. The major compounds of C. japonica seed were identified by MS/MS analysis, comparison retention time, and UV absorbance. HPLC method was validated by specificity, accuracy, precision and limit of quantification. The HPLC method showed that calibration curves for each of the three compounds have high linearity with a correlation coefficient (R2) of > 0.99%. The limits of detection (LOD) and limit of quantitation (LOQ) for camelliaside B were 0.084㎍/㎖ and 0.254㎍/㎖, respectively. Intra-day precision of camelliaside B was 0.09-0.16 RSD. The content of camelliaside B in C. japonica seed extract collected by region by standardized HPLC-DAD analysis varied from 0.279 to 2.05 mg/g. All tested parameters met the appropriate ranges designated in the guideline. Therefore, the HPLC-DAD method established in this study could provide the first step in developing health-related functional products or pharmaceuticals derived from camellia seed extract.
참고문헌 (17)
- Bondonno NP, "Higher habitual flavonoid untakes are associated with a lower incidence of diabetes", J. Nutr, 151(11), pp.3533~3542 (2021)
- Dinda B, "Dietary plant flavonoids in prevention of obesity and diabetes", Adv. Protein Chem. Struct. Biol, 120, pp.159~235 (2019)
- Imran M, "Luteolin, a flavonoid, as an anticancer agent : A review", Biomed. Pharmacother, 112, pp.108612~ (2019)
- 에 따른 개똥숙 열수추출분말의 Scopoletin 분석을 위한 HPLC 분석법 밸리데이션, "생약학회지", 2020, 78~85, pp.한국생약학회
- Ko J, "Kaempferol tri- and tetrasaccharides from Camellia japonica seed cake and their inhibitory activities against matrix metalloproteinase-1 secretion using human dermal fibroblasts", Carbohydr. Res, 495, pp.108101~ (2020)
- Lee Ju Sung, "Validation of an optimized HPLC", UV method for the quantification of flavonoids in lotus, 2020, pp.63(6), 1~6[DOI]
- 김선민, "국내 자생 동백나무(Camellia japonica L.) 추출물의 항산화 및 항미생물 활성", 한국자원식물학회지, 17(3), pp.314~322 (2004), 한국자원식물학회
- Maleki SJ, "Anti-inflammatory effects of flavonoids", Food Chem, 299, pp.125124~ (2019)
- Małgorzata Nita, "The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults", Oxidative Medicine and Cellular Longevity, 2016, pp.1~23 (2016), Hindawi Limited[DOI]
- Pal D, "Flavonoids: A powerful and abundant source of antioxidants", Int. J. Pharm. Pharm. Sci, 5, pp.95~98 (2013)
- Perez-Vizcaino F, "Research trends in flavonoids and health", J Bio Chem Phys, 646, pp.107~112 (2018)
- Pham LA, "Free radicals, antioxidants in disease and health", Int. J. Biomed Sci, 4, pp.89~96 (2008)
- Raffa D, "Recent discoveries of anticancer flavonoids", Eur. J. Med Chem, 142, pp.213~228 (2017)
- Wang X, "Enzymatic synthesis of acylated quercetin 3-O-glycosides : A review", Sheng Wu Gong Cheng Xue Bao, 37(6), pp.1900~1918 (2021)
- Wang X, "Flavonoid intake and risk of CVD : A systematic review and meta-analysis of prospective cohort studies", Br. J. Nutr, 111, pp.1~11 (2014)
- Yang S, "A novel multifunctional compound camellikaempferoside B decreases Aβ production, interferes with Aβ aggregation, and prohibits Aβ-mediated neurotoxicity and neuroinflammation", ACS. Chem. Neurosci, 7(4), pp.505~518 (2016)
- Yoon IS, "Identification of the biologically active constituents of Camellia japonica leaf and anti-hyperuricemic effect in vitro and in vivo", Int. J. Mol. Med, 39(6), pp.1613~1620 (2017)