반응표면분석법을 이용한 구절초의 Linarin, Chlorogenic Acid 추출 조건 최적화
Optimization of Linarin, Chlorogenic Acid by Response Surface Methodology of Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam.
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초록
구절초(Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam.)는 linarin, chlorogenic acid 등의 유용성분들을 함유하고 있으며, 산업적으로 이용가치가 있는 식물이다. 구절초의 산업화를 위해 유용성분인 linarin과 chlorogenic acid의 최적 추출 조건을 구하기 위해 반응표면분석을수행하였으며, Linarin의 최적 추출 조건은 에탄올 농도 100%, 추출 시간 30h, 추출 온도 75℃였다. 최적 추출 조건에서 예상된 linarin의 값은33.36 mg/g였으며 실험값은 38.33±2.66 mg/g였다. Chlorogenic acid의 최적 추출 조건은 에탄올 농도 48%, 추출 시간 30h, 추출 온도 65℃로예상된 chlorogenic acid의 함량은 42.19 mg/g였으며 실험값은 44.34±2.09 mg/g이었다. 반응표면분석법으로 도출된 최적 추출 조건을 통해구절초에서 linarin과 chlorogenic acid의 효율적인 추출이 가능함을 확인하였다.
Abstract
Chrysanthemum zawadskii var. latilobum contains useful components such as linarin and chlorogenic acid, and is a plant with industrialvalue. For the industrialization of C. zawadskii var. latilobum, response surface analysis was performed to determine the optimal extractionconditions for the useful components linarin and chlorogenic acid. The optimal extraction conditions for linarin were 100% ethanolconcentration, 30h extraction time, and 75℃ extraction temperature. The expected value of linarin under optimal extraction conditionswas 33.36 mg/g, and the experimental value was 38.33±2.66 mg/g. The optimal extraction conditions for chlorogenic acid were 48%ethanol concentration, 30h extraction time, and 65℃ extraction temperature. The expected chlorogenic acid content was 42.19 mg/g,and the experimental value was 44.34±2.09 mg/g. It was confirmed that efficient acquisition of linarin and chlorogenic acid was possiblethrough optimal extraction conditions derived by response surface analysis.
참고문헌 (25)
- Bezerra MA, "Response surface methodology(RSM)as a tool for optimization in analytical chemistry", Talanta, 75(5), pp.965~977 (2008)
- Butiuk AP, "Optimization and modeling of the chlorogenic acid extraction from a residue of yerba mate processing", J. Applied Resear. Medi. Aromatic Plants, 25, pp.100329~ (2021)
- Byun JH, "Anti-osteoarthritic mechanisms of Chrysanthemum zawadskii var. latilobum in MIA-induced osteoarthritic rats and interleukin-1β-induced SW1353 human chondrocytes", Medicina, 56(12), pp.685~ (2020)
- Chadni M, "Optimization of extraction conditions to improve chlorogenic acid content and antioxidant activity of extracts from forced witloof chicory roots", Foods, 11(9), pp.1217~ (2022)
- Im DH, "Study on increasing the saponin content and determination the optimum extraction method for saponin in Codonopsis lanceolata", J. Agri. Life Sci, 55(4), pp.21~29 (2021)
- Kang J, "Polyphenol Profiling and comparison of extraction solvents on useful component content and antioxidant activity of Rhododendron micranthum Shoot", Korean J. Plant Res, 37(2), pp.110~119 (2024)
- Kim JS, "Optimization of drying conditions for chamchwi (Aster scaber Thunb.)using the response surface methodology with a central composition design", J. Korean Soc. Food Sci. Nutr, 52(8), pp.844~855 (2023)
- Kim KM, "Quantitative analysis about phenolic compounds of dendranthema species in Korean native plants", Korean J. Pharmacog, 48(1), pp.70~76 (2017)
- Kim YJ, "Comparison of linarin content and biological activity in ethanol extraction of Chrysanthemum zawadskii", J. Korean Soc. Food Sci. Nutr, 45(10), pp.1414~1421 (2016)
- Kim YS, "Hepatoprotective effect of Chrysanthemum zawadskii extract(CZE)in experimentally induced liver damage model in vitro and in vivo", J. Korean Soc. Food Sci. Nutr, 48(2), pp.189~197 (2019)
- Kim YY, "Biological activities of linarin from Chrysanthemum zawadskii var. latilobum", Yakhak Hoeji, 45(6), pp.604~610 (2001)
- Kwon HS, "Cytotoxic flavonoids from the whole plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura", J. Life Sci, 16(5), pp.746~749 (2006)
- Lim WS, "Optimization of DPPH radical scavenging activity by response surface methodology in the ultrasonic-assisted extraction of Lespedeza bicolor Turcz", J. Agri. Life Sci, 58(2), pp.65~80 (2024)
- Lopez-Garcia E, "Coffee consumption and risk of stroke in women", Circulation, 119(8), pp.1116~1123 (2009)
- Lou Z, "Antibacterial activity and mechanism of action of chlorogenic acid", J. Food Sci, 76(6), pp.M398~M403 (2011)
- Mottaghipisheh J, "Linarin, a glycosylated flavonoid, with potential therapeutic attributes : A comprehensive review", Pharmaceuticals, 14(11), pp.1104~ (2021)
- Oh H, "Application of response surface methodology for optimization of applemango jelly processing", Korean J. Food Nutr, 35(6), pp.473~480 (2022)
- Pan H, "Optimization of extraction of linarin from Flos chrysanthemi indici by response surface methodology and artificial neural network", J. Separation Sci, 40(9), pp.2062~2070 (2017)
- Park SJ, "Optimization of roasting conditions for coffee beans by response surface methodology", Korean J Food Preserv, 18, pp.178~183 (2011)
- Rheem S, "The model-fit proportion and the coefficient of pure error variation, the measures to supplement the lack-of-fit test in response surface analysis", J. Korean Data Anal. Soc, 14(6), pp.2989~2994 (2012)
- Song JH, "Analysis on the trends of korean health functional food patent based on the medicinal plant resources", Korean Herb. Med. Inf, 8(1), pp.25~44 (2020)
- Sultana B, "Effect of extraction solvent", technique on the antioxidant activity of selected medicinal plant extracts, 2009, pp.14(6), 2167~2180
- Thom E, "The effect of chlorogenic acid enriched coffee on glucose absorption in healthy volunteers and its effect on body mass when used long-term in overweight and obese people", J. Int. Medi. Res, 35(6), pp.900~908 (2007)
- Xie G, "Optimization of the extraction conditions for Buddleja officinalis Maxim. using response surface methodology and exploration of the optimum harvest time", Molecules, 22(11), pp.1877~ (2017)
- Ye Y, "Purification process and in vitro and in vivo bioactivity evaluation of pectolinarin and linarin from Cirsium japonicum", Molecules, 27(24), pp.8695~ (2022)