인체 및 온혈 동물 기회감염 세균 Pseudomonas oryzihabitans에 의한양파 구 흑화병
Aversion Center Blackening of Onion Bulb Caused by Pseudomonas oryzihabitans, an Opportunistic Bacterial Pathogen of Humans and Warm-blood Animals
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초록
본 연구는 인체 및 온혈 동물의 기회감염 병원세균으로 알려진 Pseudomonas oryzihabitans에 의한 양파 구에 발생한 중심 흑화병의 첫 보고이다. 본 연구의 목적은 양파 구 흑화병을 일으키는 원인 병원체를 분리 및 동정하고 미생물학적 특성을 조사하는데 있다. 2021년 국내 양파 구에서소비자 혐오감을 유발할 수 있는 세균 흑화병이 관찰되었다. 흑갈색 색소가 침착된 조직과 흑색 색소 과육 조직에서 그람음성, 비포자형성, 막대형pseudomonad들이 분리되었다. 병원성 검정을 통해 형성된 인공 감염후 발생한 증상은 자연 감염과 같았고 멸균 증류수를 처리한 음성 대조구에서는흑화병이 발견되지 않았다. 병원성 검정, API (analytical profile index) 시험, MALDI-TOF MS (Whole-cell matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry), 16S rRNA 유전자 영역 염기서열분석 및 유전적 유연관계분석을 통해 원인 병원체를Pseudomonas oryzihabitans로 동정하였다. 대규모 포장 또는 수확후병으로 인한 양파 피해사례가 보고된 적은 없지만, 본 연구에서 얻은 결과는P. oryzihabitans가 잠재적으로 양파의 글로벌 생산 및 운송과 식품 안전을 위협할 수 있음을 시사한다. 또한 인체 기회감염 병원체이기 때문에양파로부터 언제든지 인체로 감염될 수 있는 위험성을 갖고 있다고 볼 수 있다.
Abstract
This study is the first report of central blackening in onion bulbs caused by Pseudomonas oryzihabitans, an opportunistic pathogenin humans and warm-blooded animals. The purpose of this study is to isolate and identify the pathogen causing onion bulb blackeningand to investigate its microbiological characteristics. In 2021, a center blackening of onion bulbs that could cause consumer disgustwas observed in onion bulbs in Korea. Gram-negative, non-spore-forming, rod-shaped pseudomonads were isolated from black pigmentedtissue of onion bulbs. Symptoms after artificial infection formed through the pathogenicity assay were the same as those of naturalinfection, and there were no symptoms in the negative control treated with sterile distilled water. The causative pathogen was identified as Pseudomonas oryzihabitans through pathogenicity assay, API (analytical profile index) test, MALDI-TOF MS (whole-cell matrix-assistedlaser desorption/ionization time-of-flight mass spectrometry), and 16S rRNA gene region sequencing and phylogenetic analysis. Althoughno cases of onion damage from large-scale field or post-harvest disease have been reported, the results obtained in this study suggestthat P. oryzihabitans could potentially pose a threat to onion global production and transportation and food safety. In addition, sinceit is an opportunistic human pathogen, it can be seen that it has a risk of being infected from onions to the human body at any time.
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