회화나무 캘러스 배양에 미치는 식물생장조절제 및 광질의 영향
Influence of Plant Growth Regulator and Light-Emitting Diodes on Formation and Induction for Callus of Styphnolobium Japonicum (L.) Schott
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본 연구는 산업화 유망 식물인 회화나무의 이차대사산물 대량생산용 캘러스 확보에 필요한 기초조건을 확인하고자 수행하였다. 기내 배양한회화나무 하배축을 2.0 mg·L-1 NAA + 0.1 mg·L-1 TDZ 배지에서 배양하였을 때 유백색의 부드러운 캘러스가 형성되었고, 캘러스 형성률 100±0.0%,캘러스 형성에 걸리는 기간 7.1±0.03일, 캘러스 무게 249.8±12.73 mg으로 가장 효과적임을 확인하였다. 또한, 하배축에서 유도한 캘러스는 백색광조건의 1.0 mg·L-1 IBA + 0.1 mg·L-1 TDZ 처리 배지에서 배양하였을 때 연노란색의 단단한 캘러스가 다량으로 형성되었고, 캘러스 생체중186±5.4 mg, 면적 40.6±1.6 mm2으로 가장 무겁고, 크기가 우수한 것을 확인하였다. 기내 배양한 캘러스 소재 추출물의 추출수율은 50% 에탄올추출 시 59%, 물 추출 시 54%로 확인하였고, 추출물 내 유효성분으로 마키아인 함량은 1.68 mg·g-1으로 검출되었다. 본 연구의 결과는 산업화식물로써 유망있는 수종인 회화나무의 기내 대량증식과 이차대사산물 생산성을 향상할 수 있는 위한 기초자료로 활용할 수 있을 것이다.
Abstract
This study was conducted to survey the basic conditions for the mass production of secondary metabolites from the industrially promisingplant, Styphnolobium japonicum (L.) Schott. Hypocotyl were cultured on media containing NAA 2.0 mg·L-1 + TDZ 0.1 mg·L-1 a milkywhite soft callus was formed, resulting in the most effective with a callus induction rate of 100±0.0%, a duration of 7.1±0.03 daysfor callus formation, and a callus weight of 249.8±12.73 mg. Callus from hypocotyl was formed a large amount of light yellow hardon media containing IBA 1.0 mg·L-1 + TDZ 0.1 mg·L-1 under fluorescent light conditions, and resulting in the most effective witha highest fresh weight of callus (186±5.4 mg) and exellent size of callus (40.6±1.6 mm2). The yield of callus extract was investigated59% (50% ethanol extraction), 55% (water extraction). It was confirmed that the maackiain was detected 1.68 mg·g-1 in callus extract. The results of this study can be used as basic data for in vitro mass propagation and enhancing for secondary metabolite productionof Styphnolobium japonicum (L.) Schott for expected for species for industrial applications.
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