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Vitek GNI+ Card에서 Vibrio alginolyticus로 잘못 동정된 3예의 경험

Misidentification as Vibrio alginolyticus with Vitek GNI+ Card in Three Cases

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초록

세 증례에서 Vitek GNI+ card를 사용한 미생물 동정검사에서 처음에는 sucrose, ornithine decarboxylase와 lysine decarboxylase, arginine dihydrolase 등의 한두가지 생화학적 반응검사 결과가 잘못 판독되어 모두 V. alginolyticus로 동정되었으나 전통적인 수기법, API 20E 검사 및 Vitek GNI+ 재검사에서 다른 Vibrio아종이나 Aeromonas 속으로 확인 동정되었다. 따라서 Vitek GNI+ card에 의한 균주 동정에서는 Vibrio 속에서 다른 아종 또는 Aeromonas 속이 Vibrio 속으로 잘못 동정될 가능성을 염두에 두어 이를 보완할 수 있는 수기법이나 다른 상품화된 동정 제품을 사용해야 할 필요성이 있다.

Abstract

The introduction of a new, fully automated system into the clinical microbiology laboratory contributes to a rapid identification of microorganisms with accurate and reliable results, but such a system requires a high cost and additional tests for identification of some species. For instance, additional tests on oxidase, indole, motility, hemolysis, and pigmentation are needed in the correct identification by using Vitek GNI+ system (bioMerieux Vitek Inc., MO, USA). In particular, Vibrioand Aeromonas species are occasionally identified incorrectly when an automated system is used, and thus conventional biochemical tests may be more reliable in the identification of such species. We experienced three cases of incorrect identification of Vibrio parahaemolyticus, Vibrio cholerae, and Aeromonas veroniibiovar sobria as Vibrio alginolyticusby using Vitek GNI+ card.

참고문헌 (25)

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