혈액 한천배지와 초콜릿 한천배지에서 ‘Spiking’ 판독에 의한Candida albicans의 신속동정
Rapid Identification of Candida albicans by ‘Spiking’ onBlood and Chocolate Agar Plates
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배경: Candida albicans는 혈액 한천배지에서 ‘spiking’ 모양을 나타내는데, 혈액 한천배지를 사용한 일차 분리배지에서의 집락 판독 시 이 현상을 확인하는 것으로 C. albicans를 신속하게 동정할 수 있어, 본 연구에서는 ‘spiking’ 모양 판독에 의한 C. albicans의 동정 결과를 평가하였다. 방법: 임상분리 균주 144균주와 표준균주 10균주를 대상으로 혈액 한천배지와 초콜릿 한천배지를 사용하여 5% CO2 배양기에서 배양하여 ‘spiking’ 모양을 확인하였고, 혼주 인 혈청을 사용하여 발아관 시험을 시행하였다. 결과: 본 연구에서 C. albicans 추정 동정을 위하여 시행한 신속검사의 민감도는 혈액 한천배지와 초콜릿 한천배지에서의 ‘spiking’ 판독이 각각 93.7%와 91.1%였고 발아관 시험은 98.7%였으며, 특이도는 모두 100%였다. 결론: 집락 판독 시 일차 분리배지인 혈액 한천배지에서 ‘spiking’ 모양 판독은 일반 검사실에서 경제적이고 신속한 C. albicans의 추정 동정에 유용할 것으로 사료되었다.
Abstract
Background: Colonial morphology of Candida albicans known as ‘spiking’ on a primary isolation blood agar plate (BAP) allows rapid and presumptive identification of C. albicans. We evaluated the ‘spiking’ appearance to identify C. albicans. Methods: A total of 144 fully identified clinical isolates of yeasts and 10 type strains of yeasts were tested. All isolates obtained from the 5% CO2 incubation on BAP and chocolate agar plate (CHOC) were macroscopically examined for the presence of an irregular margin (spiking). The germ tube test was performed by incubating test organisms in 0.5 mL of pooled human sera. Results: The sensitivity for BAP-spiking, CHOC-spiking and germ tube test were 93.7%, 91.1%, and 98.7%, respectively. The specificity for three methods was 100%. Conclusion: Use of the spiking identification on BAP can be useful for the economic and rapid presumptive identification of C.albicans in routine laboratories.
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