Evaluation of MicroScan MICroSTREP Plus Antimicrobial Susceptibility Panel for Testing Streptococcus pneumoniae
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초록
배경: 최근 여러 가지 streptococci의 항균제감수성검사가 가능한 MicroScan MICroSTREP plus panel (Dade Behring, West Sacramento, CA, USA)이 국내에 소개되었다. 저자들은 폐렴연쇄구균을 대상으로 MicroScan MICroSTREP plus panel을 이 용한 항균제감수성검사를 평가하고자 하였다. 방법: 임상 검체에서 분리된 폐렴연쇄구균 75균주를 대상으로 하였고, MicroScan MICroSTREP plus panel과 clinical and laboratory standards institute (CLSI) 미량액체배지희석법으로 penicillin, cefotaxime, ceftriaxone, meropenem, vancomycin, clindamycin, erythromycin, levofloxacin에 대한 항균제감수성검사를 실시하였다. 46균주의 폐렴연쇄구균에 대해서는 Etest를 이용하여 penicillin과 cefotaxime에 대한 항균제감수성검사를 추가로 실시하였다. 결과: CLSI 미량액체배지희석법의 결과와 2배 이내의 희석 배수의 결과를 보인 경우를 일치한 MIC 결과를 보인 것으로 간주하였고, MicroScan MICroSTREP plus panel의 전반적인 MIC 결과 일치율은 93.0%였다. 내성과 감수성의 판정에서는 minor error 11.7%, major error 0.7%, very major error 0.7%의 결과를 보였다. Etest 결과와 MicroScan MICroSTREP plus panel의 결과 사이에 큰 차이는 관찰되지 않았다. 결론: MicroScan MICroSTREP plus panel은 MIC 결과를 얻을 수 있는 액체배지희석법으로, 폐렴연쇄구균의 항균제감수성검사에서 CLSI 미량액체배지희석법과 비교하였을 때 양호한 결과를 보였다. 임상미생물검사실에서MicroScan MICroSTREP plus panel을 효과적으로 이용할 수 있을 것으로 생각한다.
Abstract
Background: The MicroScan MICroSTREP plus panel for susceptibility testing of various streptococci, including Streptococcus pneumoniae, has recently been introduced in Korea. The current study evaluated the usefulness of MicroScan MICroSTREP plus panel for antimicrobial susceptibility test of S. pneumoniae. Methods: A total of 75 clinical isolates of S. pneumoniae were tested for antimicrobial susceptibility to penicillin, cefotaxime, ceftriaxone, meropenem, vancomycin, clindamycin, erythromycin, and levofloxacin with the MicroScan MICroSTREP plus panel and clinical and laboratory standard institute (CLSI) reference broth microdilution method. For 46 of 75 isolates, additional susceptibility tests to penicillin and cefotaxime were performed with Etest. Results: The overall essential agreement of MICs (within one dilution of MICs) defined by the MicroScan MICroSTREP plus panel and reference method was 93.0%. Overall there were 11.7% minor, 0.7% major, and 0.7% very major interpretative category errors observed. The results of antibiotic susceptibility testing by Etest were similar to those obtained by the MicroScan MICroSTREP plus panel. Conclusion: The MicroScan MICroSTREP plus panel, a commercial broth microdilution method, has a comparable accuracy to CLSI broth microdilution method for the resistance testing of S. pneumonia. This panel can be used for determining susceptibilities of S.pneumoniae to a wide variety of antimicrobial agents in clinical microbiology laboratories.
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