그람양성과 그람음성세균에 대한 MicroScanⓇ Synergies plus Combo Panel과 MicroScanⓇ Conventional Combo Panel의 비교
Comparison of the MicroScanⓇ Combo Panel Synergies plus with the MicroScanⓇ Conventional Combo Panel for Diagnostic Performance of Gram-negative and Gram-positive Bacteria
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배경: MicroScan Synergies plusⓇ Combo Panels (Siemens, USA)의 그람음성막대균과 그람양성알균에 대한 동정과 항균제 감수성 검사의 임상적 유용성을 평가하기 위하여 MicroScan Conventional Combo Panels와의 일치도를 분석하였다. 방법: 그람음성막대균 104균주는 MicroScan Synergies plusⓇ Neg Combo Type 2 Panel (SINC2)과 MicroScan Neg Combo Panel Type 44 (NC44)를, 그람양성알균 100균주는 MicroScan Synergies plusⓇ Pos Combo 3 Panel (SIPC3)과 MicroScan Pos Combo 1A (PC1A)를 동시에 검사하였다. 결과: 그람음성막대균의 SINC2와 NC44의 균속과 균종 수준에서의 동정 일치율은 각각 93.3%와 92.3%였고, 그람양성알 균의 SIPC3와 PC1A의 균속과 균종 수준에서의 동정 일치율은 100%와 85.0%였다. 그람음성막대균의 SINC2와 NC44의 항균제감수성검사 결과의 일치율은 항균제별로 86.5∼100% 범위였고, 그람양성알균의 SIPC3와 PC1A의 항균제감수성검 사 결과의 항균제별 일치율은 gentamicin과 quinupristin-dalfopristin을 제외하곤 96.0% 이상이었다. 결론: MicroScan Synergies plusⓇ Combo Panel인 SINC2와 SIPC3는 MicroScan Conventional Combo Panels인 NC44와 PC1A 와의 동정과 항균제감수성검사 결과의 일치도가 높았으며 신속하게 보고할 수 있는 장점이 있었다.
Abstract
Background: To access the clinical usefulness of MicroScanⓇ Synergies plus Combo Panels (Siemens, USA) for the identification and antimicrobial susceptibility test (AST) of Gram-negative bacteria (GNB) and Gram-positive cocci (GPC), we compared MicroScanⓇ Synergies plus Combo Panels with MicroScanⓇ conventional Combo Panels. Methods: One-hundred four isolates of GNB were simultaneously tested with MicroScanⓇ Synergies plus Neg Combo Type 2 Panel (SINC2) and MicroScanⓇ Neg Combo Panel Type 44 (NC44). One-hundred isolates of GPC were simultaneously tested with MicroScanⓇ Synergies plus Pos Combo 3 Panel (SIPC3) and MicroScanⓇ Pos Combo 1A (PC1A). Results: Of the GNB isolates, agreement rate of identification between SINC2 and NC44 were 92.3% to the species level and 93.3% to the genus level. Of the GPC isolates, agreement rate of identification between SIPC3 and PC1A were 85.0% to the species level and 100% to the genus level. Of the GNB isolates, agreement rate of AST according to antimicrobial agents between SINC2 and NC44 ranged from 86.5% to 100%. Among GPC isolates, agreement rate of AST according to antimicrobial agents between SIPC3 and PC1A were higher than 96.0% with the exception of gentamicin and quinupristin- dalfopristin. Conclusion: Compared with MicroScanⓇ conventional Combo Panels (NC44, PC1A), MicroScanⓇ Synergies plus Combo Panels (SINC2, SIPC3) showed high agreement rate of identification and AST, and had the advantage of more rapid reporting.
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