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임상검체에서 분리된 칸디다 균종에서2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride 비색법을 이용한Fluconazole 항진균제 감수성 검사의 평가

Evaluation of a Colorimetric Antifungal Susceptibility Test by2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride for Fluconazolein Candida Species Isolated from Clinical Specimens

이은정|김혜란|장철훈|신정환|이자영|이정녀|김재현
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2007.10|Annals of Clinical Microbiology|제10권 제2호|pp. 90-95

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초록

배경: 임상검체에서 검출되는 다양한 칸디다 균종에서 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) 비색법을 이용한 fluconazole에 대한 항진균제 감수성 검사를 평가하고 CLSI M27-A2의 표준방법의 결과와 비교하고자 하였다. 방법: 임상검체에서 검출된 204균주의 칸디다를 연구대상으로 하였다. C. albicans 100균주, C. glabrata 45균주, C. tropicalis 28균주, C. parapsilosis 22균주 및 기타 Candida species 9균주로 시행하였다. Fluconazole 항진균제 감수성 검사는 CLSI법과 STC 비색법으로 시행하였다. 결과: CLSI 표준방법에서는 Candida 204균주 모두 양성 대조군에서 증식하였으나 STC가 포함된 양성 대조군에서는 Candida 26균주 (C. albicans 6균주와 C. tropicalis 20균주)가 증식하지 않았다. STC를 이용한 비색법의fluconazole 최소억 제농도를 육안 및 흡광도로 판정하였을 때 양성대조군에서 증식하지 않은 26균주를 제외한 178 균주 중 171주 (96.1%)와 160주 (89.9%)에서 CLSI 표준방법과 두 단계 희석범위 내의 일치율을 보였다. C. albicans에 대한 STC 육안판정은 표준방 법과 비교하였을 때 1, 2, 3단계 희석범위에서 각각 76.6, 92.6, 및 95.8%의 일치율을 보였다. STC 비색법의 두 가지 판정법(육안 및 흡광도)은 178균주 중 170주 (95.5%)에서 두 단계 희석범위 내의 값을 나타내어 높은 일치율을 보였 다. 결론: STC를 이용한 비색법은 C. tropicalis를 제외한 칸디다 균종에서 CLSI 표준방법과 높은 일치율을 보였으며 결과판 정이 객관적이고 용이하였다.

Abstract

Background: The aims of this study were to evaluate the colorimetric antifungal susceptibility test to fluconazole using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) for various Candida species isolated from clinical specimens and to compare the results with those of the CLSI M27-A2 standard method. Methods: The fluconazole MICs for 204 clinical Candida isolates consisting of 100 C. albicans, 45 C. glabrata, 28 C. tropicalis, 22 C. parapsilosis, and 9 other Candida species were determined by the CLSI and STC colorimetric methods. Results: All 204 Candida strains were grown on the growth control wells of CLSI standard plates, but 26 Candida strains (6 C. albicans and 20 C. tropicalis) were not grown on those containing STC. Therefore, those 26 Candida strains were excluded from the comparison of MICs in this report. Overall, the STC visual and spectrophotometric readings of fluconazole MICs showed 96.1% (N=171) and 89.9% (N=160) accordance with those obtained by the CLSI standard method within 2 dilutions, respectively. The STC visual reading of C. albicans showed 76.6, 92.6, and 95.8% accordance with the CLSI standard method within 1, 2, and 3 dilutions, respectively. The agreement between the two endpoint determinations of the STC colorimetric method (visual and spectrophotometric readings) was excellent, with 170 of the 178 MICs within 2 dilutions. Conclusion: The STC colorimetric method to determine the MIC for Candida species except C. tropicalis showed high levels of agreement with CLSI method. And also, it is useful with objective and easy interpretation.

참고문헌 (27)

  1. "Investigational antifungal agents", 21(S), pp.165~174 (2001)
  2. "The exciting future of antifungal therapy", 19, pp.897~914 (2000)
  3. "Bloodstream infections due to Candida species: SENTRY antimicrobial surveilance program in North America and Latin America, 1997-1998", 44, pp.747~751 (2000)
  4. "Antifungal drug resistance in pathogenic fungi", 36(S1), pp.119~128 (1998)
  5. "Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard-second edition" (2002), Wayne, PA; NCLS
  6. "Novel fluorescent broth microdilu-tion method for fluconazole susceptibility testing of Candida albi-cans", 39, pp.2708~2712 (2001)
  7. "Laboratory evaluation of antifungal agents a com-parative study of five imidazole derivatives of clinical importance", pp.749~61
  8. "Candida albicans proteinase as a virulence factor in the pathogenesis of Candida infections", pp.539~42
  9. "Comparison of visual and spectropho-tometric methods of broth microdilution MIC end point deter-mination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing Candida isolates", 46, pp.2477~2481 (2002)
  10. "Comparison of a photometric method with standardized methods of antifungal susceptibility testing of yeasts", 35, pp.2878~2882 (1997)
  11. "Fluconazole disk diffusion procedure for determing susceptibility of Candida species", 34, pp.2154~2157 (1996)
  12. "Multisite reproducibility of the Etest MIC method for antifungal suscepti-bility testing of yeast isolates", 34, pp.1691~1693 (1996)
  13. "Collaborative study of the NCLS and flow cytometry methods for antifungal susceptibility testing of Candida albicans", 42, pp.2249~2251 (2004)
  14. "Flow cytometry antifungal susceptibility testing of pathogenic yeasts other than Candida albicans and comparison with the NCLS broth microdilution test", 44, pp.2752~2758 (2000)
  15. "Rapid susceptibility testing of Candida albicans by flow cytometry", 35, pp.358~363 (1997)
  16. "Comparison of two alternative microdilution procedures with the National Comitee for Clinical Laboratory Standards reference macrodilution method M27-P for in vitro testing of fluconazole- resistant and -susceptible isolates of Candida albicans", 33, pp.3154~3158 (1995)
  17. "In vitro susceptibility of Cryptococcus neoformans isolates to five anti-fungal drugs using a colorimetric system and the reference micro-broth method", 45, pp.645~649 (2000)
  18. "Evaluation of a novel colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates"
  19. "Comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates", 33, pp.2660~2664 (1995)
  20. "Clinical evaluation of the ASTY colorimetric microdilution panel for antifungal susceptibility testing", 36, pp.2609~2612 (1998)
  21. "Multicenter evaluation of a colorimetric microplate antimyco-bacterial susceptibility test: comparative study with the NCLS M24-P", 44, pp.456~464 (1996)
  22. "Deter-mination of antimycobacterial activities of fluoroquinolones against clinical isolates of Mycobacterium tuberculosis: comparative deter-mination with egg-based Ogawa and agar-based Middlebrok 7H10 media", 71, pp.453~458 (1996)
  23. "Evalua-tion of a colorimetric antifungal susceptibility test by using 2,3- diphenyl-5-thienyl-(2)-tetrazolium chloride", 48, pp.4457~4459 (2004)
  24. - 2H-tetrazolium hydroxide (XT) colorimetric method with the standardized National Comittee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents, , 1998, 1450~1452
  25. "Standardization of antifungal susceptibility testing and clinical relevance", 36(S1), pp.68~78 (1998)
  26. "Antifungal susceptibility testing: technical advances and potential clinical applications", 24, pp.776~784 (1997)
  27. "Development of interpretive breakpoints for antifungal susceptibility testing: conceptual framework and analysis of in vitro-in vivo correlation data for fluconazole, itraconazole, and candida infections", 24, pp.235~247 (1997)

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