본문 바로가기
KERD
논문KCI 등재

Collagen-coated Polyethylene Terephthalate 디스크 Plate와 Shell Vial을 이용한 Chlamydophila pneumoniae 배양법의 비교

Comparison of Collagen-coated Polyethylene Terephthalate Disc Plate and Shell Vial Culture Method for the Isolation of Chlamydophila pneumoniae

김미혜|이원길
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2010.06|Annals of Clinical Microbiology|제13권 제2호|pp. 73-78

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Cell cultureCollagen-coated PET disc plateShell vialChlamydophila pneumoniae

초록

배경: 호흡기 감염의 중요한 원인 중 하나인 Chlamydophila pneumoniae의 정확한 진단을 위해서는 세포 배양을 통해 C. pneumoniae의 검출을 확인하는 것이 중요하다. 최근 콜라겐이 코팅된 polyethylene terephthalate 디스크(PET 디스크)를polyester plate 용기에 넣어 사용하는 제품(CorePath, Daegu, Korea)이 개발되어 세포 배양에 이용되고 있다. 이에 저자는PET 디스크 plate의 유용성을 평가하고 기존의 방법과 비교하기 위해 본 연구를 실시하였다. 방법: C. pneumoniae 배양이 의뢰된 환자 46명에서 채취한 29개의 객담과 17개의 혈액 검체를 대상으로 shell vial과 PET 디스크 plate (4-well)를 이용하여 배양하였고 결과가 상이한 경우 microimmunofluorescence (MIF) 결과를 비교하였다. 결과: HeLa-229 세포단층은 모든 shell vial과 PET 디스크 plate에서 관찰되었으며, 양성인 경우 C. pneumoniae 배양 결과세포질 내에 하나의 둥근 봉입체가 형광 염색되었다. 두 검사법에서 판정이 일치하는 경우는 양성 20예와 음성 22예,총 42예로 91.3%의 우수한 일치율(k=0.826)을 보였다. 결론: PET 디스크 plate 배양법은 shell vial법의 결과와 비교하여 우수한 결과 일치율을 보였으며, 또한 plate와 디스크를다루는 데에도 어려움이 없어 shell vial의 cover glass를 대신하여 PET 디스크 plate를 사용하여도 좋을 것으로 판단된다. [대한임상미생물학회지 2010;13:73-78]

Abstract

Background: Chlamydophila pneumoniae is one of the major respiratory infectious pathogens and can be accurately diagnosed by cell culturing. The author performed this study to compare the usefulness of the collagen-coated polyethylene terephthalate (PET)disc culture method and that of the shell vial method. Methods: Twenty-nine sputums and 17 blood specimens collected from 46 patients for C. pneumoniae culture were inoculated into HeLa-229 cell monolayers cultured in shell vials and polyester plates. After incubation, they were stained using the indirect immunofluorescent method with genus-specific FITC-conjugated anti-chlamydia antibody. When both results were inconsistent, microimmunofluorescence results were used. Results: HeLa-229 cells successfully formed monolayers in shell vials and collagen-coated PET plates in all cases. Positive inclusion bodies in HeLa-229cells of shell vials and PET plates for C. pneumoniae culture were similarly stained with the indirect immunofluorescent method. Both methods showed consistent results with 20 positive and 22 negative cases. The total agreement between the PET plate and shell vial was excellent (91.3%, k=0.826). Conclusion: The collagen-coated PET disc culture method showed highly consistent results with that of the shell vial method, and no technical differences were experienced between the two methods. Therefore, the author concluded that the shell vial method could be replaced by the PET plate method for detection of C. pneumoniae. (Korean J Clin Microbiol 2010;13:73-78)

참고문헌 (26)

  1. Hammerschlag MR, "The intracellular life of chlamydiae", Semin Pediatr Infect Dis, 13, pp.239~248 (2002)
  2. Everett KD, "Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms", Int J Syst Bacteriol, 49, pp.415~440 (1999)
  3. Chi EY, "Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR", J Bacteriol, 169, pp.3757~3763 (1987)
  4. Kuo CC, "Campbell LA, Grayston JT. Chlamydia pneumoniae (TWAR)", Clin Microbiol Rev, 8, pp.451~461 (1995)
  5. Blasi F, "Chlamydophila pneumoniae", Clin Microbiol Infect, 15, pp.29~35 (2009)
  6. Wong YK, "Circulating Chlamydia pneumoniae DNA as a predictor of coronary artery disease", J Am Coll Cardiol, 34, pp.1435~1439 (1999)
  7. Layh-Schmitt G, "Evidence for infection with Chlamydia pneumoniae in a subgroup of patients with multiple sclerosis", Ann Neurol, 47, pp.652~655 (2000)
  8. Balin BJ, "Identification and localization of Chlamydia pneumoniae in the Alzheimer's brain", Med Microbiol Immunol, 187, pp.23~42 (1998)
  9. Cascina A, "Meloni F. Cutaneous vasculitis and reactive arthritis following respiratory infection due to Chlamydia pneumoniae: report of a case", Clin Exp Rheumatol, 20, pp.845~847 (2002)
  10. Hahn DL, "Serologic markers for Chlamydia pneumoniae in asthma", Ann Allergy Asthma Immunol, 84, pp.227~233 (2000)
  11. Dowell SF, "Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada)", Clin Infect Dis, 33, pp.492~503 (2001)
  12. Kumar S, "Acute respiratory infection due to Chlamydia pneumoniae: current status of diagnostic methods", Clin Infect Dis, 44, pp.568~576 (2007)
  13. Kim KS, "A study for culture condition of Chlamydia pneumoniae", Korean J Clin Pathol, 17, pp.137~145 (1997)
  14. Frobes BA, "Bailey & Scott's Diagnostic Microbiology. 12th ed", pp.756~759 (2007), Mosby
  15. Kuo CC, "Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR", J Clin Microbiol, 26, pp.812~815 (1988)
  16. Maass M, "Growth in serum-free medium improves isolation of Chlamydia pneumoniae", J Clin Microbiol, 31, pp.3050~3052 (1993)
  17. Pruckler JM, "Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations", J Clin Microbiol, 37, pp.3399~3401 (1999)
  18. Tjhie JH, "Improvement of growth of Chlamydia pneumoniae on HEp-2 cells by pretreatment with polyethylene glycol in combination with additional centrifugation and extension of culture time", J Clin Microbiol, 35, pp.1883~1884 (1997)
  19. Kazuyama Y, "A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA", J Clin Microbiol, 35, pp.1624~1626 (1997)
  20. Roblin PM, "Use of HEp-2 cells for improved isolation and passage of Chlamydia pneumoniae", J Clin Microbiol, 30, pp.1968~1971 (1992)
  21. Chirgwin K, "Infection with Chlamydia pneumoniae in Brooklyn", J Infect Dis, 163, pp.757~761 (1991)
  22. Boman J, "Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA", J Infect Dis, 175, pp.1523~1526 (1997)
  23. Verkooyen RP, "Evaluation of PCR, culture, and serology for diagnosis of Chlamydia pneumoniae respiratory infections", J Clin Microbiol, 36, pp.2301~2307 (1998)
  24. Apfalter P, "Application of blood-based polymerase chain reaction for detection of Chlamydia pneumoniae in acute respiratory tract infections", Eur J Clin Microbiol Infect Dis, 20, pp.584~586 (2001)
  25. Miyashita N, "Evaluation of the diagnostic usefulness of real-time PCR for detection of Chlamydophila pneumoniae in acute respiratory infections", J Infect Chemother, 13, pp.183~187 (2007)
  26. Poppert S, "Detection and differentiation of Chlamydiae by fluorescence in situ hybridization", Appl Environ Microbiol, 68, pp.4081~4089 (2002)

관련 논문AI추천

호흡기 검체에서 Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila 검출을 위한 EuDxTM-PN MLC Detection 키트의 평가성흥섭, 이미경, 조아라, 지현영대한임상미생물학회 · Annals of Clinical Microbiology2017 · 제20권 제4호호흡기바이러스 검출을 위한 배양법, 직접면역형광법 및 다중역전사효소연쇄반응법의 비교윤귀현, 조지현대한진단검사의학회 · Laboratory Medicine Online2011 · 제1권 제4호Comparison of Sputum and Nasopharyngeal Swab Specimens for Molecular Diagnosis of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila김미나, 김혜원, 노신애, 안동희, 우준희, 이미영, 정용필, 조민철대한진단검사의학회 · Annals of Laboratory Medicine2012 · 제32권 제2호호흡기 검체에서 폐렴의 세균성 원인균 검출을 위한 다중 PCR 검사의 임상적 평가이미애, 정채림, 정화순대한임상미생물학회 · Annals of Clinical Microbiology2010 · 제13권 제1호Shell Vail Culture를 이용한 단순포진 바이러스 분리강정옥, 박해일, 이규택, 최태열대한진단검사의학회 · Annals of Laboratory Medicine2003 · 제23권 제5호Multiplex Real-time PCR Assay for the Detection of all Chlamydia Species and Simultaneous Differentiation of C. psittaci and C. pneumoniae in Human Clinical SpecimensChande Aroon T., Conley Andrew B., Diaz Maureen H., Gaines Anna, Norris Emily, Rishishwar Lavanya, Winchell Jonas M., Wolff Bernard J., Yang Eungi대한진단검사의학회 · Annals of Laboratory Medicine2023 · 제43권 제4호

이 간행물의 다른 논문 (8건)

실시간-염기순서기반증폭(Real-Time Nucleic Acid Sequence-based Amplification)을 통한 장바이러스 검출김의종, 김홍빈, 박성섭, 박경운, 성기형, 송정한, 전선희, 송상훈, 최은화pp. 53-582003∼2007년 한 대학병원 혈액배양에서 분리된 장알균의 항균제 저항성 및 Vancomycin 내성 장알균 균혈증에 관여하는 위험인자 분석이희주, 서진태, 김명희, 박경선, 박태성pp. 59-67개정된 Penicillin Breakpoints (CLSI M100-S18)에 따른 폐렴알균의 Penicillin 감수성률 변화서일혜, 송영희, 김경희, 박순호, 안정열, 박필환pp. 68-72화상 전문병원에서의 Candida tropicalis를 포함한 칸디다 균종 분포와 Azole 감수성김태형, 이미경, 이용성, 이규만pp. 79-84다중 PCR법으로 진단한 Verotoxin 생성 Escherichia coli O157:H7 출혈성 장염인 영아 1예김경모, 조민철, 김미나, 노신애, 조해선pp. 85-89
원문 보기