Practical guide for the diagnosis of helminth ova in stools
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In the age of globalization of infectious diseases, qualified personnel is needed for thediagnosis of parasitic diseases in the laboratory. This review aimed to introduce the methodsfor stool examination and identification of helminth eggs for the diagnosis of helminthicinfections in laboratory and field surveys. The formalin-ether sedimentation technique (FEST)and the Kato-Katz egg counting technique (KKECT) are mainly described as representativestool examinations. The FEST is somewhat complicated and troublesome, but it is useful fordifferentiating small trematode eggs from opisthorchiid and heterophyid flukes. KKECT isuseful in field surveys of large populations in areas endemic for soil-transmitted helminthiases. Helminth eggs are divided into four groups based on the presence or absence of theoperculum and embryo. The eggs of Clonorchis sinensis and heterophyid flukes includingMetagonimus spp. are relatively small and contain an operculum and an embryo (miracidium). Meanwhile, eggs of diphyllobothriid tapeworms, echinostomatid flukes, Paragonimuswestermani, Fasciola hepatica, and Fasciolopsis buski are relatively larger, operculated, andcontain germ cells and yolks instead of the embryo. The eggs of cyclophyllidian tapeworms,Taenia spp. and Hymenolepis spp., and blood flukes, Schistosoma spp., are embryonatedbut do not have an operculum. Nematode eggs have no operculum and embryo, but thoseof hookworms and pinworms sometimes have developed larvae inside. This review providesvaluable insights into the methods of stool examination and helminth egg identification forthe diagnosis of helminthic infections in the laboratory and field surveys.
Abstract
In the age of globalization of infectious diseases, qualified personnel is needed for thediagnosis of parasitic diseases in the laboratory. This review aimed to introduce the methodsfor stool examination and identification of helminth eggs for the diagnosis of helminthicinfections in laboratory and field surveys. The formalin-ether sedimentation technique (FEST)and the Kato-Katz egg counting technique (KKECT) are mainly described as representativestool examinations. The FEST is somewhat complicated and troublesome, but it is useful fordifferentiating small trematode eggs from opisthorchiid and heterophyid flukes. KKECT isuseful in field surveys of large populations in areas endemic for soil-transmitted helminthiases. Helminth eggs are divided into four groups based on the presence or absence of theoperculum and embryo. The eggs of Clonorchis sinensis and heterophyid flukes includingMetagonimus spp. are relatively small and contain an operculum and an embryo (miracidium). Meanwhile, eggs of diphyllobothriid tapeworms, echinostomatid flukes, Paragonimuswestermani, Fasciola hepatica, and Fasciolopsis buski are relatively larger, operculated, andcontain germ cells and yolks instead of the embryo. The eggs of cyclophyllidian tapeworms,Taenia spp. and Hymenolepis spp., and blood flukes, Schistosoma spp., are embryonatedbut do not have an operculum. Nematode eggs have no operculum and embryo, but thoseof hookworms and pinworms sometimes have developed larvae inside. This review providesvaluable insights into the methods of stool examination and helminth egg identification forthe diagnosis of helminthic infections in the laboratory and field surveys.
참고문헌 (35)
- Seo BS, "Clinical parasitology", pp.360~370 (1978), Ilchokak
- Beaver PC, "Clinical pathophysiology", pp.733~748 (1984), Lea & Febiger
- Chai JY, "Seo and Lee’s clinical parasitology", pp.22~27 (2011), Seoul National University Press
- Mohd-Zain SN, "Stray animals and human defecation are sources of soil-transmitted helminth eggs in playgrounds in Peninsular Malaysia", J Helminthol, 89, pp.740~747 (2015)
- Campos MC, "Helminth eggs as parasitic indicators of fecal contamination in agricultural irrigation water, biosolids, soils, and pastures", Biomedica, 38, pp.42~53 (2018)
- Chai JY, "Paleoparasitological research on ancient helminth eggs and larvae in the Republic of Korea", Parasites Hosts Dis, 61, pp.345~387 (2023)
- A national survey of the prevalence of intestinal parasitic infections in Korea in 2012, "Korea Centers for Disease Control and Prevention", Korea National Institutes of Health, ~ (2013)
- Seo BS, "An epidemiological study on clonorchiasis and metagonimiasis in riverside areas of Korea", Korean J Parasitol, 19, pp.137~150 (1981)
- Cho SH, "Prevalence of clonorchiasis in the southern endemic areas of Korea in 2006", Korean J Parasitol, 46, pp.133~137 (2008)
- Jeong YI, "Prevalence of Clonorchis sinensis infection among residents of five major rivers in the Republic of Korea", Korean J Parasitol, 54, pp.215~219 (2016)
- Yoo WG, "Current status of Clonorchis sinensis and clonorchiasis in Korea : epidemiological perspectives integrating data from humans and intermediate hosts", Parasitology, 149, pp.1296~1305 (2022)
- Lee MR, "Status of helminthic infections in residents of river basins in the Republic of Korea over 10 Years(2011-2020)", Korean J Parasitol, 60, pp.187~193 (2022)
- KAHP annual report, "Korean Association for Health Promotion (KAHP)", Korean Association for Health Promotion, 49~ (2021)
- Ju JW, "Current status of the elimination project for intestinal parasitic diseases in areas endemic for food-borne trematodiasis", Week Health Dis, 11, pp.426~432 (2017)
- Chai JY, "Effect of mass drug administration with a single dose of Albendazole on Ascaris lumbricoides and Trichuris trichiura infections among schoolchildren in the Yangon Region, Myanmar", Korean J Parasitol, 58, pp.195~200 (2020)
- Yong TS, "Prevalence of intestinal helminths among Cambodian inhabitants(2006-2011)", Korean J Parasitol, 52, pp.661~666 (2014)
- Eom KS, "Prevalence of helminthic infections among inhabitants of Lao PDR", Korean J Parasitol, 52, pp.51~56 (2014)
- Rim HJ, "Prevalence of intestinal parasite infection on a national scale among primary school children in Laos", Parasitol Res, 91, pp.267~272 (2003)
- Kim JY, "Effectiveness of mass drug administration for neglected tropical diseases in schoolchildren in Zanzibar, Tanzania", Korean J Parasitol, 58, pp.109~119 (2020)
- Garcia LS, "Clinical microbiology procedure handbook", pp.2265~ (2010), American Society for Microbiology Press
- Garcia LS, "Practical guidance for clinical microbiology laboratories : diagnosis of parasites in the gastrointestinal tract", Clin Microbiol Rev, 31, pp.e00025~17 (2017)
- Palmieri JR, "Emerging need for parasitology education : training to identify and diagnose parasitic infections", Am J Trop Med Hyg, 84, pp.845~846 (2011)
- McCarthy JS, "Research agenda for helminth diseases in humans : diagnostics for control and elimination programs", PLoS Negl Trop Dis, 6, pp.e1601~ (2012)
- Boonyong S, "Highthroughput detection of parasites and ova in stool using a fully automatic digital feces analyzer, Orienter model fa280", Parasites Vectors, 17, pp.13~ (2024)
- Lefaure B, "Evaluation of a flotation adapted Parasep® for stool ova and parasite examination", J Parasitol, 105, pp.480~483 (2019)
- Sarirah M, "Comparison of mini-flotac and Kato-Katz methods for detecting soil-transmitted helminth eggs in 10% formalin-preserved stools stored for > 12 months", Trop Biomed, 36, pp.677~686 (2019)
- Khanna V, "A comparative study of formalin-ethyl acetate sedimentation technique and Mini Parasep® solvent-free method in the rapid diagnosis of intestinal parasites", Trop Parasitol, 8, pp.29~32 (2018)
- World Health Organization, "Bench aids for the diagnosis of intestinal parasites" (2019), WHO
- Ryoo S, "Standard-and large-sized eggs of Trichuris trichiura in the feces of schoolchildren in the Yangon region, Myanmar : morphological and molecular analyses", Parasites Hosts Dis, 61, pp.317~324 (2023)
- Soares FA, "Historical review of parasite recovery techniques for detection in human stools", Rev Soc Bras Med Trop, 53, pp.e20190535~ (2020)
- Inácio SV, "Automated diagnosis of canine gastrointestinal parasites using image analysis", Pathogens, 9, pp.139~ (2020)
- Jim´enez B, "Helminth egg automatic detector(HEAD) : improvements in development for digital identification and quantification of helminth eggs and their application online", Exp Parasitol, 217, pp.107959~ (2020)
- Alva A, "Mathematical algorithm for the automatic recognition of intestinal parasites", PLoS One, 12, pp.e0175646~ (2017)
- Jimenez B, "Identification and quantification of pathogenic helminth eggs using a digital image system", Exp Parasitol, 166, pp.164~172 (2016)
- Yang YS, "Automatic identification of human helminth eggs in microscopic fecal specimens using digital image processing and an artificial neural network", IEEE Trans Biomed Eng, 48, pp.718~730 (2001)