Verification of the Mycoplasma IST3 for urogenital mycoplasma culture in comparison to the Mycoplasma IST2
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Background: Unlike the Mycoplasma IST2 kit (bioMérieux), the Mycoplasma IST3 kit hasbeen updated to comply with the standardized antimicrobial susceptibility test (AST) methodfor Ureaplasma spp. (Up) and Mycoplasma hominis (Mh). We aimed to verify the use of theMycoplasma IST3 kit for genital mycoplasma cultures. Methods: From September 2023 to January 2024, the R1 medium remaining after inoculationwith IST2 was refrigerated until the next day. For IST2-positive samples, 300 μL of residual R1medium was inoculated into the IST3. Species identification, enumeration, and AST resultsobtained using IST3 were compared with those obtained using IST2. Results: A total of 48 IST2-positive samples were inoculated into IST3, including 35, 1, and12 Up-only, Mh-only, and both Up- and Mh-positive samples, respectively. Among Up-onlysamples, 2.8%, 91.4%, and 100.0% were susceptible to ciprofloxacin, tetracycline, anderythromycin, respectively. With IST3, 45 (93.8%) samples grew genital mycoplasmas; 42(89.4%) of the 47 Up-positive samples and 6 (46.2%) of the 13 Mh-positive samples showedgrowth of the same organisms. All seven samples that failed to grow Mh were from mixedcultures, of which four Mh concentrations of < 104/mL. Up was susceptible to levofloxacin,tetracycline, and erythromycin at the rates of 64.3 %, 88.1 %, and 95.2 %, respectively. Conclusion: IST3 showed good performance in detecting genital Mycoplasma except forits tendency to not detect Mh of low concentrations in mixed cultures. IST3 is preferable toIST2 because it can accurately screen for erythromycin resistance in Up and reduce falseresistances for fluoroquinolone.
Abstract
Background: Unlike the Mycoplasma IST2 kit (bioMérieux), the Mycoplasma IST3 kit hasbeen updated to comply with the standardized antimicrobial susceptibility test (AST) methodfor Ureaplasma spp. (Up) and Mycoplasma hominis (Mh). We aimed to verify the use of theMycoplasma IST3 kit for genital mycoplasma cultures. Methods: From September 2023 to January 2024, the R1 medium remaining after inoculationwith IST2 was refrigerated until the next day. For IST2-positive samples, 300 μL of residual R1medium was inoculated into the IST3. Species identification, enumeration, and AST resultsobtained using IST3 were compared with those obtained using IST2. Results: A total of 48 IST2-positive samples were inoculated into IST3, including 35, 1, and12 Up-only, Mh-only, and both Up- and Mh-positive samples, respectively. Among Up-onlysamples, 2.8%, 91.4%, and 100.0% were susceptible to ciprofloxacin, tetracycline, anderythromycin, respectively. With IST3, 45 (93.8%) samples grew genital mycoplasmas; 42(89.4%) of the 47 Up-positive samples and 6 (46.2%) of the 13 Mh-positive samples showedgrowth of the same organisms. All seven samples that failed to grow Mh were from mixedcultures, of which four Mh concentrations of < 104/mL. Up was susceptible to levofloxacin,tetracycline, and erythromycin at the rates of 64.3 %, 88.1 %, and 95.2 %, respectively. Conclusion: IST3 showed good performance in detecting genital Mycoplasma except forits tendency to not detect Mh of low concentrations in mixed cultures. IST3 is preferable toIST2 because it can accurately screen for erythromycin resistance in Up and reduce falseresistances for fluoroquinolone.
참고문헌 (18)
- Waites KB, "Manual of Clinical Microbiology" (2023), American Society of Microbiology
- Chang YS, "Genital mycoplasma in the newborn infants : colonization, prevalence and clinical significance", J Korean Pediatr Soc, 30, pp.1084~1094 (1996)
- Koh E, "Antimicrobial susceptibilities of Ureaplasma urealyticum and Mycoplasma hominis in pregnant women", Korean J Clin Microbiol, 12, pp.159~162 (2009)
- Chung HK, "Association of genital mycoplasmas infection in women who had preterm delivery and outcomes in premature infants", Korean J Obstet Gynecol, 55, pp.158~165 (2012)
- D'Inzeo T, "Comparison of Mycoplasma IES, Mycofast Revolution and Mycoplasma IST2 to detect genital mycoplasmas in clinical samples", J Infect Dev Ctries, 11, pp.98~101 (2017)
- Park HR, "Usefulness of the Mycofast test(MYCOFASTⓇEvolution 2)for the diagnosis of nongonococcal genitourinary infections", Korean J Urol, 47, pp.1117~1123 (2006)
- Wen X, "Tetracyclines resistance in Mycoplasma and Ureaplasma urogenital isolates derived from human : a systematic review and meta-analysis", Ann Clin Microbiol Antimicrob, 22, pp.83~ (2023)
- Song J, "Prevalence and antibiotics resistance of Ureaplasma species and Mycoplasma hominis in Hangzhou, China, from 2013 to 2019", Front Microbiol, 13, pp.982429~ (2022)
- "Mycoplasma IST2. Diagnosis of urogenital mycoplasma (culture, identification, enumeration, and susceptibility testing). Package insert. bioMérieux REF 42505", ~
- "Mycoplasma IST3. Diagnosis of urogenital mycoplasma (culture, identification, enumeration, and susceptibility testing). Package insert. bioMérieux REF 422083", ~
- Clinical and Laboratory Standards Institute, "Methods for antimicrobial susceptibility testing for human mycoplasmas" (2011), CLSI
- Boostrom I, "Evaluation of the Mycoplasma IST3 urogenital mycoplasma assay in an international multicentre trial", J Antimicrob Chemother, 76, pp.3175~3182 (2021)
- Choe HS, "Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections : comparison with currently available methods", Int J Infect Dis, 17, pp.e1134~40 (2013)
- Min SK, "Evaluation of clinical sample for Accupower UU Real-Time PCR kit", Korean J Urogenit Tract Infect Inflamm, 9, pp.99~103 (2014)
- Chang J, "Prevalence and antimicrobial susceptibility of genital Mycoplasmataceae in Korean women : correlation between phenotypic test and resistance genes", Ann Clin Microbiol, 19, pp.13~19 (2016)
- Kweon OJ, "Prevalence and antimicrobial susceptibility of Mycoplasma hominis, Ureaplasma urealyticum and Ureaplasma parvum in individuals with or without symptoms of genitourinary infections", Lab Med Online, 6, pp.79~87 (2016)
- Lee JY, "Prevalence and antimicrobial susceptibility of Mycoplasma hominis and Ureaplasma species in nonpregnant female patients in South Korea indicate an increasing trend of pristinamycin-resistant isolates", Antimicrob Agents Chemother, 64, pp.e01065~20 (2020)
- Chung HY, "A case of erythromycinresistant Ureaplasma urealyticum meningitis in a premature infant", Korean J Lab Med, 27, pp.46~49 (2007)