국내 양돈장에서 돼지글래셔병을 동반한 PMWS에 대한 M+ parapac®의 방어효과
Efficacy of M+ parapac® on protection against PMWS with Glasser's disease
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The purpose of this study was to evaluate the efficacy and cross-protection of serovar 12 against serovar 4 or 5 of H parasuis with M+Parapac®. A total of 141 piglets from 2(A and B) farms were used and divided into experimental group and control group in each farm. Farm A has been detected H parasuis serovar 12, whereas farm B has been detected H parasuis serovar 4 or 5 from post-weaned pigs with PMWS. The piglets were vaccinated intramuscularly with 2.0ml of M+Parapac® in experimental group or normal saline in control group at 1 week of age. A same booster dose was given at 3 weeks of age. In order to value the antibody titer to H parasuis using by tube agglutination test, blood samples were collected from piglets at the aged of 1 week, 6 and 14 weeks. In experimental group and control group, the average antibody titers were 32.5±21.0, 114.5±34.0, 98.1±55.4 and 32.9±18.6, 25.8±36.9, 746.7±1,215.8 at the aged of 1 week, 6 and 14 weeks, respectively. The cumulative clinical sign indexes by standard guideline of Schering-Plough Animal Health Corp were 486 and 1,069, respectively. The average daily gains and feed conversion rates were 0.553±0.016kg and 0.492±0.004kg, and 1.99 and 2.24, respectively. The average gross lesion scores were 1.0±0.8 and 1.9±0.6, respectively. According to these results, the M+ Parapac® containing H parasuis serovar 12 may be induce circulating antibodies that cross-react with serovar 4 or 5 and have a protection of PMWS with H parasuis.
Abstract
The purpose of this study was to evaluate the efficacy and cross-protection of serovar 12 against serovar 4 or 5 of H parasuis with M+Parapac®. A total of 141 piglets from 2(A and B) farms were used and divided into experimental group and control group in each farm. Farm A has been detected H parasuis serovar 12, whereas farm B has been detected H parasuis serovar 4 or 5 from post-weaned pigs with PMWS. The piglets were vaccinated intramuscularly with 2.0ml of M+Parapac® in experimental group or normal saline in control group at 1 week of age. A same booster dose was given at 3 weeks of age. In order to value the antibody titer to H parasuis using by tube agglutination test, blood samples were collected from piglets at the aged of 1 week, 6 and 14 weeks. In experimental group and control group, the average antibody titers were 32.5±21.0, 114.5±34.0, 98.1±55.4 and 32.9±18.6, 25.8±36.9, 746.7±1,215.8 at the aged of 1 week, 6 and 14 weeks, respectively. The cumulative clinical sign indexes by standard guideline of Schering-Plough Animal Health Corp were 486 and 1,069, respectively. The average daily gains and feed conversion rates were 0.553±0.016kg and 0.492±0.004kg, and 1.99 and 2.24, respectively. The average gross lesion scores were 1.0±0.8 and 1.9±0.6, respectively. According to these results, the M+ Parapac® containing H parasuis serovar 12 may be induce circulating antibodies that cross-react with serovar 4 or 5 and have a protection of PMWS with H parasuis.
참고문헌 (25)
- Straw BE, "Disease of Swine", pp.681~690 (2006), Blackwell Publishing
- Enøe C, "Infectious and rearing-system related risk factors for chronic pleuritis in slaughter pigs", Prev Vet Med, 54(4), pp.337~349 (2002)
- Rautiainen E, "Regional eradication of Mycoplasma hyopneumoniae from pig herds and documentation of freedom of the disease", Acta Vet Scand, 42(3), pp.355~364 (2001)
- Oliveira S, "Development of a PCR test to diagnose Haemophilus parpasuis infection", J Vet Diagn Invest, 13, pp.495~501 (2001)
- Cu HP, "Prevalence of Haemophilus sp infection in the upper respiratory tract of pigs and some characteristics of the isolates", Khoa Hoc Ky Thuat Thu Y, 5, pp.88~93 (1998)
- Hjarre A, "Enzootic virus pneumonia and Glӓsser's disease of swine", Adv Vet Sci, 4, pp.235~ (1958)
- Baehler JF, "Haemophilus parasuis chez le pore", Scweiz. Arch. Tierhielk, 116, pp.183~188 (1974)
- 이난주, "Haemophilus parasuis균의 항원 특성과 분리균의 혈청학적 분류에 관한 연구", 박사, pp.1994
- Choi YK, "Retrospective analysis of etiologic agents associated with respiratory diseases in pigs", Can Vet J, 44(9), pp.735~737 (2003)
- Bak YB, "A pathological study on the cases of Glӓsser's disease", Res. Rep Kor Ins Anim Sci, 2, pp.71~79 (1982)
- Lee RJ, "Biochemical properties of H parasuis and serological classification of the iso-lates", Kor J Vet Publ Hlth, 18, pp.127~138 (1994)
- Won HK, "Characterization of Haemophilus parasuis isolates from swine in Korea", 석사, pp.2003
- Kang ZW, "Studies on Haemo-philus parasuis in pigs", 석사, pp.2004
- Rapp-Gabrielson VJ, "Efficacy and duration of immunity of the Haemophilus parasuis fractions of Suvaxyn RespiFend MH", HPS, 1996, pp.14, 300~
- Takahashi K, "A cross-protection experiment in pigs vaccinated with Haemophilus parasuis serovars 2 and 5 bacterins, and evaluation of a bivalent vaccine under laboratory and field conditions", J Vet Med Sci, 63(5), pp.487~491 (2001)
- Bak H, "Protection of vaccinated pigs against experimental infection with homologous and hetero-logous Haemophilus parasuis", Vet Rec, 151, pp.502~505 (2002)
- Collins CH, "Collins and Lyne'x Microbiological Method", pp.118~120 (1995), Oxford: Butterworth-Heinemann
- Gunnarsson A, "Serologic studies on porcine strains of Haemophilus parahaemolyticus(Pleuropneumoniae): Agglutination reaction", Am J Vet Res, 38, pp.1111~1114 (1977)
- 석호봉, "돼지 다발성장막염 및 관절염의 원인균 조사 및 분리균의 병발성에 관한 연구", 대한수의공중보건학회지, 17(1), pp.1~12 (1993)
- Jayappa H, "Evaluation of efficacy of Haemophilus parasuis bacterin in providing protecion against heterologous challenge", Allen D Leman Swine Conf Proc, 31, pp.61~ (2004)
- Jayappa H, "Cross protecion of a Haemophilus parasuis serotype 12 vaccine(ParapacⓇ) against serotype 4", Am Assoc Swine Vet, pp.181~185 (2006)
- Kocur G, "Haemo-philus parasuis immunity in swine after vaccination", Vet Med, 92, pp.1~7 (1997)
- Olvera A, "Genotypic diversity of field strains", Am Soc Microbiol, 72, pp.3984~3992 (2006)
- Solano GI, "Effect of porcine reproductive and respiratory syndrome virus infection on the clearance of Haemophilus parasuis by porcine alveolar macrophages", Can J Vet Res, 62(4), pp.251~256 (1998)
- Karpiński TM, "Comparison of the values of selected reagents for the diagnosis of swine brucellosis. I. Preparation and standardization of antiglobulin serum", Pol Arch Weter, 22(1), pp.29~40 (1979)