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소에서 비임신 및 임신 상태의 난소 형태와 혈중 progesterone 농도 변화에 의한 조기 임신진단

A study on the early pregnancy diagnosis by changing of plasma progesterone concentration and morphology of ovary in pregnancy and non-pregnancy cows

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In order to evaluate conception rate of Hanwoo in northwestern region of Gyeongsang-nam-do, we investigated conception rate and reduction of reproductive disorder rate after artificial insemination(AI) in 1,000 heads of breeding cows. This study showed that 80.9% of cows were classified as fertility after 1st and 2nd AI. For a accurate pregnancy diagnosis with practicing ovariectomy and histeotomy, we comparatively investigated each of 80 slaugh-tered cows, including 30 of non-pregnancy, and used enzyme-linked immunosorbent assay (ELISA) for estimation of plasma progesterone concentration and serum luteal hormone. The mean diameter of non-pregnant corpus luteum is 18.9±4.2×15.6±3.6㎜ and that of pregnant corpus luteum is 22.5±2.7×18.7±2.9㎜. This indicates that corpus luteum is more developed in the ovary of pregnant than non-pregnant cows(P<0.05) The diameter of pregnant corpus luteum according to the stage of pregnancy showed 21.3±2.4×18.4±2.6㎜ in early stage(1-3month), 23.4±2.8×19.1±2.7㎜ in middle stage(4-6 month) and 22.8±3.0×18.8±2.4㎜ in last stage(7-9 month). This indicates that corpus luteum in middle and last stage is more significantly developed than that of early stage(P<0.05). The mean plasma progesterone concentration of cows showing size of non-pregnant corpus luteum was 4.58±0.92ng/㎖ and that of pregnant corpus luteum 8.26±0.98ng/㎖. Thus, it was more significantly increased in pregnant corpus luteum(P<0.02).. However, it was low to 0.58±0.39ng/㎖. in estrus(corpus albicans). The plasma progesterone concentration according to gestation period was high in proportion to the degree of development in corpus luteum and more significantly increased (P<0.05) and maintained in middle and last state than early state. The concentration was sharply decreased to 0.56±0.32ng/㎖ at parturition. As a consequence, we can practice the early pregnancy diagnosis by confirming non-pregnancy when the mean plasma progesterone con- centration is below 1ng/㎖ 19 to 22 days after AI and this can be available to diagnose reproductive disorder.

Abstract

In order to evaluate conception rate of Hanwoo in northwestern region of Gyeongsang-nam-do, we investigated conception rate and reduction of reproductive disorder rate after artificial insemination(AI) in 1,000 heads of breeding cows. This study showed that 80.9% of cows were classified as fertility after 1st and 2nd AI. For a accurate pregnancy diagnosis with practicing ovariectomy and histeotomy, we comparatively investigated each of 80 slaugh-tered cows, including 30 of non-pregnancy, and used enzyme-linked immunosorbent assay (ELISA) for estimation of plasma progesterone concentration and serum luteal hormone. The mean diameter of non-pregnant corpus luteum is 18.9±4.2×15.6±3.6㎜ and that of pregnant corpus luteum is 22.5±2.7×18.7±2.9㎜. This indicates that corpus luteum is more developed in the ovary of pregnant than non-pregnant cows(P<0.05) The diameter of pregnant corpus luteum according to the stage of pregnancy showed 21.3±2.4×18.4±2.6㎜ in early stage(1-3month), 23.4±2.8×19.1±2.7㎜ in middle stage(4-6 month) and 22.8±3.0×18.8±2.4㎜ in last stage(7-9 month). This indicates that corpus luteum in middle and last stage is more significantly developed than that of early stage(P<0.05). The mean plasma progesterone concentration of cows showing size of non-pregnant corpus luteum was 4.58±0.92ng/㎖ and that of pregnant corpus luteum 8.26±0.98ng/㎖. Thus, it was more significantly increased in pregnant corpus luteum(P<0.02).. However, it was low to 0.58±0.39ng/㎖. in estrus(corpus albicans). The plasma progesterone concentration according to gestation period was high in proportion to the degree of development in corpus luteum and more significantly increased (P<0.05) and maintained in middle and last state than early state. The concentration was sharply decreased to 0.56±0.32ng/㎖ at parturition. As a consequence, we can practice the early pregnancy diagnosis by confirming non-pregnancy when the mean plasma progesterone con- centration is below 1ng/㎖ 19 to 22 days after AI and this can be available to diagnose reproductive disorder.

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