본문 바로가기
KERD
논문KCI 등재

PFGE를 이용한 Brucella abortus의 유전형별

Genotyping of Brucella abortus by PFGE

김영환|김성국|엄현정|김순태|장영술|조민희|고영활
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2009.09|한국동물위생학회지|제32권 제3호|pp. 257-264

0

조회

0

다운로드

2

인용

0

참고문헌

주제분류수의학

초록

Abstract

The process of subtyping is important epidemiologically for recognizing outbreaks of infection. Pulsedfield gel electrophoresis (PFGE) is considered the “gold standard” of molecular typing methods and a method of seperating large DNA molecules. In our study, Electrophoresis of DNA digested with the restriction endonuclease Xba I, Xho I and Smi I produced fragment profiles. Digestion of genomic DNA with Xba I and Xho I provided results that not distinguished among field strains of Brucella abortus which tested in study. But Smi I restriction enzyme resulted in two PFGE pattern consisting of 13-15 bands that ranged in size from 33 to 668 standard marker. Analysing DNA fingeprinting software, cluster analysis showed 93.75% similar in two PFGE pattern.

참고문헌 (30)

  1. Alton GG, "Laboratory techniques in brucellosis, 2nd Ed", WHO, 11~63 (1975)
  2. Allardet-servent A, "DNA polymorphism in strains of the genus brucella", J Bacterol, 170(10), pp.4603~4607 (1988)
  3. Beran GW, "Handbook of zoonoses" (1994), CRC Press
  4. Brenner DT, "Bergey’s mannual of systematic bacteriology", Springer Press, 2, pp.370~389 (2005)
  5. "Standardized laboratory protocol for molecular subtyping of Escherchia coli O157:H7,non-typhoidal salmonella sero-types, and Shigella sonnei by PFGE"[URL]
  6. Chain PSG, "Whole-genome analyses of specia-tion events in pathogenic brucellae", Infect Immun, 73(12), pp.8353~8361 (2005)
  7. Chu G, "Separation of large DNA molecules by contour-clamped homogeneous electric fields", Science, 234(4783), pp.1582~1585 (1986)
  8. Cloeckaert A, "Restric-tion site polymorphism of the genes encoding the major 25kDa and 36kDa outer-membrane proteins of brucella", Micro-biology, 141(9), pp.2111~2121 (1995)
  9. Cloeckaert A, "Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp", Res Microbiol, 151(3), pp.209~216 (2000)
  10. DelVecchio VG, "The genome sequence of the facultative intracellular patho-gen Brucella melitensis", PNAS, 99(1), pp.443~448 (2002)
  11. Ficht TA, "Genetic variation at the omp2 porin locus of the brucellae:Species-specific mar-kers", Mol Microbiol, 4(7), pp.1135~1142 (1990)
  12. Foster G, "A review of brucella sp. infection of sea mammals with particular emphasis on isolates from Scotland", Vet Microbiol, 90, pp.563~580 (2002)
  13. Halling SM, "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis", J Bacteriol, 187(8), pp.2715~2726 (2005)
  14. Hunter SB, "Establishment of a universal size standard strain for use with the pulsenet standardized pulsed-field gel electro-phoresis protocol: converting the national databases to the new size standard", J Clin Micro-biol, 43(3), pp.1045~1050 (2005)
  15. Jensen AE, "Genomic fingerprinting and development of a dendrogram for Brucella spp. isloated from seals,porpoises, and dolphins", J Vet Diagn Invest, 11, pp.152~157 (1999)
  16. Jensen AE, "Application of pulsed-field gel electrophoresis for differentiation of vaccine strain RB51 from field isolates of Brucella abortus from cattle, bison, and elk", Am J Vet Res, 56(3), pp.308~312 (1995)
  17. Jesen AE, "Determination of stability of Brucella abortus RB51 by use of genomic fingerprint, oxidative metabolism,and colonial morphology and differentiation of strain RB51 from B. abortus isolates from bison and elk", J Clin Microbiol, 34(3), pp.628~633 (1996)
  18. Moreno E, "Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class proteobacteria", J Bacteriol, 172(7), pp.3569~3576 (1990)
  19. Moreno E, "Brucella evolution and taxonomy", Vet Microbiol, 90(1), pp.209~227 (2002)
  20. McPeek FD, "Separation of large DNA molecules by modified pulsed fieldgradient gel electro-phoresis", Anal Biochem, 156(2), pp.274~285 (1986)
  21. Nielsen K, "Animal brucellosis", pp.1~453 (1990), CRC Press
  22. Olson MV, "Separation of large DNA molecules by pulsed-field gel electrophoresis. A review of the basic phenomenology", J Chromator, 470(2), pp.377~383 (1989)
  23. Paulsen IT, "The Brucella suis genome reveals funda-mental similarities between animal and plant pathogens and symbionts", PNAS, 99(20), pp.13148~13153 (2002)
  24. Ridler AL, "Demonstration of polymorphism among Brucella ovis field isolates by pulsed-field gel electrophoresis", Vet microbiol, 108, pp.69~74 (2005)
  25. Schwartz, D. C, "Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis", Cell, 37, pp.67~75 (1984)
  26. Tenover FC, "Interpreting chromosomal DNA restric-tion patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing", J Clin Microbiol, 33(9), pp.2233~2239 (1995)
  27. Timoney JF, "Hagan and Bruner’s Microbiology and infectious disease of domestic animals", pp.135~152 (1988), Cornell University Press
  28. Verger JM, "Taxonomy of genus brucella", Ann Ins Pasteur Microbiol, 138(2), pp.235~238 (1985)
  29. Verstreate DR, "Comparison of sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles and antigenic related-ness among outer membrane proteins of 49 Brucella abortus strains", Infect Immun, 46(1), pp.182~187 (1984)
  30. Vizcaino N, "DNA polymorphism in the genus brucella", Microbes Infect, 2(9), pp.1089~1100 (2000)

관련 논문AI추천

Pulsed-field gel electrophoresis(PFGE)를 이용한 Listeria monocytogenes의 molecular typing김연하, 김주영, 신방우, 양윤모, 이정학, 진경선, 채희선한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호MLVA를 이용한 경북지역 소에서 분리된 Brucella abortus의 유전형별김성국, 김영환, 이홍영, 최성균, 최정혜한국동물위생학회 · 한국동물위생학회지2011 · 제34권 제3호경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적 특성 분석김성국, 김영환, 엄현정, 이양수, 장성준, 조민희, 홍현표한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호경북지방 소에서 분리한 Brucella abortus의 생화학적 특성김성국, 김영환, 박청규, 이영주, 조민희한국동물위생학회 · 한국동물위생학회지2009 · 제32권 제2호Bacteriological detection of Brucella abortus and its characterization by PCR in the sporadic outbreak of bovine brucellosis in Gyeonggi province김혜성, 심항섭, 양수정, 우종태, 이성식한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제2호임상검체로부터 분리된 Acinetobacter baumannii에서 Metallo-β-lactamase 유전자 검출과 Pulsed Field Gel Electrophoresis의 형별김신무, 김은철, 최수연대한임상검사과학회 · 대한임상검사과학회지2003 · 제35권 제2호

이 간행물의 다른 논문 (13건)

포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 Ⅰ. 혈청학적 결과, 형광항체검사 및 RT-PCR 검사김영훈, 한정희, 김문pp. 177-187포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 Ⅱ. 임상증상, 병리조직학적 검사 및 면역조직학적 검사김영훈, 한정희, 김문pp. 189-200돼지유행성설사병(PEDV) 생독 ․ 사독백신의 면역형성 비교연구권미순, 이지영, 이은미, 임정철, 서형석, 조현웅, 허부홍pp. 201-207충남 서산태안지역에서 사육중인 송아지의 코로나바이러스 감염률 조사남이현, 이미성, 강형주, 전동민, 이재봉, 육심용, 한우수pp. 209-214Brittany Spaniel에서 Proteus mirabilis에 의한 유방염 발생: 증례고원석, 곽길한, 추금숙, 송희종, 이정원pp. 215-218
원문 보기