소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성
Characterization and isolation of shiga toxin-producing Escherichia coli from Bovine feces and Carcass
1
조회
0
다운로드
4
인용
0
참고문헌
초록
Abstract
Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1〔2, 8.4%〕], stx2〔3, 12.6%〕and stx1 and stx2〔19, 87.5%〕). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.
참고문헌 (25)
- 곽효선, "국내 유통식품에서 분리된 verotoxin 생성 Escherichia coli의 특성", 식품위생안전학회지, 15(3), pp.241~247 (2000)
- 고주언, "도체표면의 분변오염과 verotoxin 생성Escherichia coli O157:H7 분리에 관한연구", 한국식품위생학회지, 12(1), pp.78~82 (1997)
- 김영부, "Polymerase chain reaction 법 및 혈중항체를 이용한 vero 독소생성대장균의 검출", 대한미생물학회지, 33(1), pp.99~110 (1998)
- 전무형, "도축돈 장분변으로부터 Shiga Toxin-Producing Escherichia coli의 분리와 성상", 대한수의학회지, 44(4), pp.551~559 (2004), 대한수의학회
- 이봉주, "송아지 설사분변으로부터 Shiga toxin-producingEscherichia coli 의 분리 및 특성규명", 대한수의학회지, 46(2), pp.135~142 (2006), 대한수의학회
- 정희곤, "독소원성대장균이 생산하는 장독소의 검색을 위한 RPLA 법과 PCR기법의 감도비교", 한국환경위생학회지, 25(1), pp.6~9 (1999)
- Aidar UL, "Serotypes, virulence genes, and intimin types of shiga toxin-producing Escherichia coli (STEC) and nteropathogenic E. coli (EPEC) isolated from calves in Sao paulo, Brazil", Int J Food Microbiol, 115(3), pp.297~306 (2007)
- Beutin L, "Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals", J Clin Microbiol, 31(9), pp.2483~2488 (1993)
- Clarke SC, "Virulence of enteropathogenic Escherichia coli, a global pathogen", Clin Microbiol Rev, 16(3), pp.365~378 (2003)
- Cookson AL, "Isolation, characterization and epidermiological accessment of shiga toxin producing Escherichia coli isolates from Newzealand", J Clin Microbiol, 44(5), pp.1863~1866 (2006)
- Furuhata K, "Prevalence and serotypes of verocytotoxinproducing Escherichia coli (VTEC) isolates from diary cattle", Jpn Assoc Infect Dis, 73(5), pp.445~501 (1999)
- Fukuyama M, "Isolation and serotypes of vero toxin-producing Escherichia coli (VTEC) from pigions and crows", Jpn Assoc Infect Dis, 77(1), pp.5~9 (2003)
- Gautom RK, "Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organism in 1 day", J Clin Microbiol, 35(11), pp.2977~2980 (1997)
- Jenkins C, "Distribution of the saa gene in strains of shiga toxinproducing Escherichia coli of human and bovine origins", J Clin Microbiol, 41(4), pp.1775~1778 (2003)
- Kamali MA, "Infection by verocytotoxin-producing Escherichia coli", Clin Microbiol Rev, 2(1), pp.15~38 (1998)
- Khan A, "Antibiotic resistance, virulence gene, and molecular profiles of shiga toxin-producing scherichia coli isolates from diverse sources in Calcutta, India", J Clin Microbiol, 40(6), pp.2009~2015 (2002)
- Matushek MG, "Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis", J Clin Microbiol, 34(10), pp.2598~2600 (1996)
- Meng J, "Antibiotic resistance of Escherichia coil O157:H7 and O157:NM isolated from animals, food and humans", J Food Prot, 61(11), pp.1511~1514 (1998)
- National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals : Approved standard", NCCLS, M31~A2 (2002)
- Paton AW, "Direct detection and characterization of shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa", J Clin Microbiol, 40(1), pp.271~274 (2002)
- Pradal N, "Prevalence and characterization of shiga toxinproducing Escherichia coli isolated from cattle, food,and children during a one-year prospective study in france", J Clin Microbiol, 38(3), pp.1023~1031 (2000)
- Schroeder CM, "Antimicrobiol resistance of Escherichia coli O157 isolated from humans, cattle, swine and food", Appl Environ Microbiol, 68(2), pp.576~581 (2002)
- Tenover FC, "Interpreting chromosomal DNA restriction patterns produced by Pulsed-Field Gel Electrophoresis : Criteria for bacterial strain typing", J Clin Microbiol, 33(7), pp.2233~2239 (1995)
- Threfall EJ, "The emergene and spread of antibiotic resistance in foodborne bacteria", Int J Food Microbiol, 62(1), pp.1~5 (2000)
- Toma C, "Distribution of putative adhesins in different seropathotypes of shiga toxin-producing Escherichia coli", J Clin Microbiol, 42(11), pp.4937~4946 (2004)