The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea
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Streptococcus equi subsp. zooepidemicus(S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP(S. zooepidemicus protective protein) gene, CNE(collagen binding protein) gene and ISR(16s rRNA intergenic spacer region) gene using ABI Prism 3,130 ×l Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.
Abstract
Streptococcus equi subsp. zooepidemicus(S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP(S. zooepidemicus protective protein) gene, CNE(collagen binding protein) gene and ISR(16s rRNA intergenic spacer region) gene using ABI Prism 3,130 ×l Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.
참고문헌 (27)
- Anzai T, "Polymerase chain reaction restriction fragment length polymorphism analysis of the SzP gene of Streptococcus zooepidemicus isolated from the respiratory tract of horses", Am J Vet Res, 63, pp.1298~1301 (2002)
- Asbury AC, "Immunoglobulin in uterine secretion of mares with differing resistance to endometritis", Therigenology, 14, pp.299~308 (1980)
- Bain AM, "The role of infection in infertility in the thoroughbred mare", Vet Rec, 78, pp.168~173 (1966)
- Bannerman DD, "Increased levels of LPS-binding protein in bovine blood and milk following bacterial lipopolysaccharide challenge", J Dairy Sci, 86, pp.3128~3137 (2003)
- Bienert A, "Bacteriological findings for endodontical and apical molar dental diseases in the horse", Dtsch Tierarztl Wochenschr, 110, pp.358~361 (2003)
- 김재훈, "더러브렛 말의 Streptococcus equi subsp. zooepidemicus 감염에 의한 복막염", 대한수의학회지, 47(3), pp.315~319 (2007), 대한수의학회
- Seong-kyoon Choi, "더러브렛 씨암말의 생식기내 세균의 분포 및 항생제 감수성 양상", 한국임상수의학회지, 24(1), pp.19~25 (2007), 한국임상수의학회
- Eigner U, "Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems", J Clin Microbiol, 43, pp.3829~3834 (2005)
- Frontoso R, "Retrospective study of bacterial isolates and their antimicrobial susceptibilities in equine uterine during fertility problems", Res Vet Sci, 64, pp.1~6 (2008)
- Harkness JE, "The biology and medicine of rabbits and rodents. 2nd ed", pp.173~175 (1983), Lea and Febiger
- Hirota KI, "Construction and validation of parentage testing for thoroughbred horses by 53 single nucleotide polymorphisms", J Vet Med Sci, 72, pp.719~726 (2010)
- Hyytia-Trees E, "Multilaboratory validation study of standardized multiple-locus variable-number tandem repeat analysis protocol for shiga toxin-producing Escherichia coli O157: a novel approach to normalize fragment size data between capillary electrophoresis platforms", Foodborne Pathog Dis, 7, pp.129~136 (2010)
- Jeffcott LB, "An assessment of wastage in thoroughbred racing from conception to 4 years of age", Equine Vet J, 14, pp.185~198 (1982)
- Gil-jae Cho, "Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing", Journal of Veterinary Science, 7(1), pp.63~67 (2006), 대한수의학회
- Lindstedt BA, "Harmonization of the multiple-locus variable-number tandem repeat analysis method between Denmark and Norway for typing Salmonella Typhimurium isolates and closer examination of the VNTR loci", J Appl Microbiol, 102, pp.728~735 (2007)
- McKinnon AO, "Equine reproduction", pp.246~253 (1993), Lea & Rebiger
- Miragaya MH, "Endometritis, salpingitis and fertilisation rates after mating mares with a history of intrauterine lumenal fluid accumulation", Equine Vet J Suppl, 25, pp.109~112 (1997)
- Ozgur NY, "Contagious equine metritis in Turkey: first isolation of Taylorella equigenitalis from mares", Vet Rec, 149, pp.120~122 (2001)
- Pycock JF, "Inflammatory components in uterine fluid from mares with experimentally induced bacterial endometritis", Equine Vet J, 22, pp.422~425 (1990)
- Quinn PJ, "Clinical Veterinary Microbiology. 9th ed", pp.127~136 (1993), Mosby
- Rober SJ, "Veterinary obstetrics and genital disease. 2nd ed", pp.135~136 (1971), Theriogenology Edward bros
- Roszel JF, "Equine endometrial cytology", Vet Clin North Am Equine Pract, 4, pp.247~262 (1988)
- Welsh RD, "The siginificance of Streptococcus zooepidemicus in the horse", Equine practice, 6, pp.6~16 (1984)
- Widders PR, "Quantitation of the immunoglobulins in reproductive tract secretions of the mare", Res Vet Sci, 37, pp.324~330 (1984)
- Widders PR, "Effect of cycle stage on immunoglobulin concentrations in reproductive tract secretions of the mare", J Reprod Immunol, 7, pp.233~242 (1985)
- Wingfield Digby NJ, "Results of concurrent bacteriological and cytological examinations of the endometrium of mares in routine stud farm practice 1978-1981", J Reprod Fertil Suppl, 32, pp.181~185 (1981)
- Younan M, "Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia", J Vet Med B Infect Dis Vet Public Health, 52, pp.142~146 (2005)