제주지역에서 사육중인 더러브렛 말 호흡기로부터 분리된 병원성 Streptoccus spp.의 생화학성상 및 약제감수성 양상
Biochemical characteristics and antimicrobials susceptibility of pathogenic Streptoccus spp. isolated from respiratory tract of Thoroughbred horses in Jeju, Korea
0
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
This study carried out to investigate the pathogenic Streptococcus spp. isolated from respiratory tract of Thoroughbred in Jeju province. The specimens were collected from nasal mucosa using a culture swab from 113 Thoroughbred horses. Suspected colonies were selected onto blood and MacConkey agar plate, and identified by standard biochemical properties using Vitek 2 system and PCR method. In this study, we isolated S. equi(n=6), S. zooepidemicus(n=31), S. equisimilis(n=5), S. dysgalactiae (n=2), S. agalctiae(n=1), non identified b-hemolytic Streptococcus spp.(n=1) from Thoroughbred horses. In antimicrobial susceptibility test, it showed a high sensibility in the most of antimicrobial except for neomycin, streptomycin, spectinomycin, erythromycin and clindamycin. These results will provide the basic information to establish control measures for the treatment and prevention of respiratory disease by pathogenic Streptococcus spp. in Thoroughbred horses in Korea.
Abstract
This study carried out to investigate the pathogenic Streptococcus spp. isolated from respiratory tract of Thoroughbred in Jeju province. The specimens were collected from nasal mucosa using a culture swab from 113 Thoroughbred horses. Suspected colonies were selected onto blood and MacConkey agar plate, and identified by standard biochemical properties using Vitek 2 system and PCR method. In this study, we isolated S. equi(n=6), S. zooepidemicus(n=31), S. equisimilis(n=5), S. dysgalactiae (n=2), S. agalctiae(n=1), non identified b-hemolytic Streptococcus spp.(n=1) from Thoroughbred horses. In antimicrobial susceptibility test, it showed a high sensibility in the most of antimicrobial except for neomycin, streptomycin, spectinomycin, erythromycin and clindamycin. These results will provide the basic information to establish control measures for the treatment and prevention of respiratory disease by pathogenic Streptococcus spp. in Thoroughbred horses in Korea.
참고문헌 (25)
- 우희연, "폐렴구균의 항균제 감수성 검사를 위한 VITEK-2 system의 평가", 대한임상병리학지, 21, pp.129~134 (2001)
- Seong-kyoon Choi, "더러브렛 씨암말의 생식기내 세균의 분포 및 항생제 감수성 양상", 한국임상수의학회지, 24(1), pp.19~25 (2007), 한국상수의학회
- Alber J, "Multiplex polymerase chain reaction for identification and differentiation of Streptococcusequi subsp. zooepidemicus and Streptococcusequi subsp. equi", J Vet Med B Infect Dis Vet Public Health, 51, pp.455~458 (2004)
- Arthur RM, "Respiratory problems in the racehorse", Vet Clin North Am Equine Pract, 6, pp.179~196 (1990)
- Bagust TJ, "A review of viral infections of horses", Aust Vet J, 48, pp.520~523 (1972)
- 최성균, "RAPD Analysis and Antimicrobial Susceptibility of Streptococcus equi subsp. zooepidemicus Isolated from Thoroughbred Horses", 생명과학회지, 20(5), pp.649~654 (2010), 한국생명과학회
- "Performance standards for antimicrobial disk susceptibility test; approved standard. 10th ed" (2009)[URL]
- Defang GN, "Induction of neutralizing antibodies to Hendra and Nipah glycoproteins using a Venezuelan equine encephalitis virus in vivo expression system", Vaccine, 29, pp.212~220 (2010)
- Elton D, "Facing the threat of equine influenza", Equine Vet J, 43, pp.250~258 (2011)
- Giles C, "Prevalence of equine adenovirus antibodies I in horses in New South Wales, Australia", Vet Microbiol, 143, pp.401~404 (2010)
- Hoffman AM, "Techniques for sampling the respiratory tract of horses", Vet Clin North Am Equine Pract, 13, pp.463~745 (1997)
- Imagawa H, "Isolation of reovirus type 3 from foals", Nippon Juigaku Zasshi, 51, pp.652~655 (1989)
- Imperi M, "A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae", J Microbiol Methods, 80, pp.212~214 (2010)
- Luque I, "Molecular typing and anti-microbial susceptibility of clinical isolates of Streptococcus equi ssp. zooepidemicus from equine bacterial endometritis", J Vet Med B Infect Dis Vet Public Health, 53, pp.451~454 (2006)
- MacLachlan NJ, "Equine viral arteritis", Adv Exp Med Biol, 581, pp.429~433 (2006)
- Mason DE, "Respiratory emergencies in the adult horse", Vet Clin North Am Equine Pract, 10, pp.685~702 (1994)
- Newton JR, "Molecular epidemiology of Streptococcus zooepidemicus infection in naturally occurring equine respiratory disease", Vet J, 175, pp.338~345 (2008)
- Silvia Preziuso, "Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR", Journal of Veterinary Science, 11(1), pp.67~72 (2010), 대한수의학회
- Pusterla N, "Surveillance programme for important equine infectious respiratory pathogens in the USA", Vet Rec, 169, pp.12~ (2011)
- Quinn PJ, "Clinical Veterinary Microbiology. 9th ed", pp.127~136 (1993), Mosby
- Reed SM, "Equine herpesvirus 1 and 4", Vet Clin North Am Equine Pract, 20, pp.631~642 (2004)
- Stephen M, "Equine Internal Medicine. 3rd ed", pp.290~313 (2009), Saunders
- Timoney JF, "The pathogenic equine streptococci", Vet Res, 35, pp.397~409 (2004)
- Whitworth JM, "Lancefield group F and related streptococci", J Med Microbiol, 33, pp.135~151 (1990)
- Wood JL, "Association between respiratory disease and bacterial and viral infections in British racehorses", J Clin Microbiol, 43, pp.120~126 (2005)
