서울지역 교육시설 및 공원 내 토양 중 분원성대장균군 오염실태
Survey of fecal coliform contamination in sands of educational facilities and parks in Seoul
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Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coli-form in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most fre-quently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics us-ing disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramy-cin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was high-ly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.
Abstract
Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coli-form in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most fre-quently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics us-ing disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramy-cin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was high-ly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.
참고문헌 (30)
- 환경부, "토지 이용 용도별 토양오염기준 및 복원기준 마련을 위한 연구", ~ (2003)
- 어린이 놀이터 바닥재 환경유해인자 조사 및 개선방안 마련, "환경부", ~ (2008)
- 환경부, "먹는물 수질공정 시험법", ~ (2013)
- 환경부, "먹는물 수질기준 및 검사등에 관한 규칙", 환경부령, ~ (2014)
- Badura A, "Prevalence, antibiotic resistance oatterns and molecular characterization Escherichia coli from Austrian sandpit", Envion Pollut, 194, pp.24~30 (2014)
- Bowen AB, "Invasive Enterobacter sakazakii disease in infants", Emerg Infect Dis, 12, pp.1185~1189 (2006)
- Chandra M, "A single step multiplex PCR for identification of six diarrheagenic E. coli pathotypes and Salmonella", Int J Med Microbiol, 303, pp.210~216 (2013)
- CLSI, "Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement", ~ (2012)
- Hwang KH, "Contaminaion status on fecal coliforms of drinking water in seoul", Report of SIHE, 41, pp.266~271 (2005)
- Jason W, "Comparative genomics of pathogenic Escherichia coli", pp.21~43 (2013), Academic press
- Johnson JR, "Phylogenetic and pathotypic similarities between Escherichia coli isolates from urinary tract infections in dogs and extraintestinal infections in humans", J Infect Dis, 183, pp.897~906 (2001)
- Mi-Kyoung Jung, "Prevalence and Thermal Stability of Enterobacter sakazakii fromUnprocessed Ready-to-Eat Agricultural Products and Powdered InfantFormulas", Food Science and Biotechnology, 15(1), pp.152~157 (2006), 한국식품과학회
- 김동수, "광주지역 어린이놀이터 토양오염 실태 조사연구", 한국폐기물자원순환학회지, 26(2), pp.168~174 (2009), 한국폐기물자원순환학회
- Kasia PK, "Recretional water quality response to filtering barrier at a great lake beach", J Environ manage, 129, pp.635~641
- 김홍태, "유통되는 쇠고기에서 분리한 대장균의 항생제 내성 조사ㆍ연구", 한국가축위생학회지, 31(1), pp.17~29 (2008), 한국가축위생학회
- Kim KA, "A study on pollution of spring in Incheon area", Kor J Sani, 22, pp.35~50 (2007)
- Kuzina LV, "Isolation and identification of bacteria associated with adult laboratory mexican fruit flies, Anastrepha ludens(Diptera : Tephritidae)", Curr Microbiol, 42, pp.290~294 (2001)
- Lee YO, "Comparative studies on detecting methods for fecal indicators", J Korean Soc on water environment & water wastewater, pp.414~419 (2006)
- 이준복, "서울시내 어린이 놀이터 토양오염도 평가", 한국환경보건학회지, 32(4), pp.249~253 (2006), 한국환경보건학회
- Lim SK, "Antimicrobial resistance of commensal bacteria isolated from food-producing animals II. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from pig fecal samples", Kor J Vet Pub Health, 31, pp.31~39 (2007)
- Luna GM, "Extraintestinal Escherichia coli Carrying Virulence Genes in Coastal Marine Sediments", App Environ Microbiol, 76, pp.5659~5668 (2010)
- Matsuo J, "Prevalence of fecal contamination in sandfits in public parks in Sapporo city, Japan", Vet Parasitol, 128, pp.115~119 (2005)
- Mudryk Z, "Detection of antibiotic resistant bacteria inhabiting the sand of non-recreational marine beach", Marine pollution Bull, 60, pp.207~214 (2010)
- Muytijens HL, "Enterobacter sakazakii meningitis in neonates causative role of fomula", Pediatr Infect. Dis J, 9, pp.372~373 (1990)
- Park HK, "Distribution of coliform and fecal coliform bacteria in lake paldang", J Korean Soc on Water Environment & Water Wastewater, pp.661~663 (2009)
- 박지은, "지표수에서의 분변오염지표세균 (대장균군) 검출방법의 비교연구", 한국물환경학회지, 22(6), pp.1052~1059 (2006), 한국물환경학회
- Percival SL, "Microbiology of Water-borne Diseases", pp.71~90 (2004), ELSEVIER
- Şengür G, "The examination of intestinal flora and parasites in dogs and the role of the contamination of the playgrounds sand with feces", Türk Mikrobiyol Cem Derg, 35, pp.57~66 (2005)
- Smith JL, "Extraintestinal pathogenic Escherichia coli", Foodborne Pathog Dis, 4, pp.134~163 (2007)
- Whitman RL, "Microbes in beach sands:integrating environment, ecology and public health", Rev Environ Sci Biotecnol, 13, pp.329~368 (2014)