Comparison of the non-invasive diagnostic methods, stool antigen test and PCR assay, for Helicobacter felis detection in dogs
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The aim of the present study was to compare the non-invasive methods for the diagnosis of H. feliswith HpSA kit-based detection method and H. felis-specific PCR assay with dog’s stool samples withoutsacrifice. Male Beagle dogs (n=6) were infected with H. felis ATCC 49179 (1.0×109 CFU/dog) by intragastricinoculation two times at 3-day intervals, and the stool specimens of dogs were collected 1, 3,5, 7, 14, 21 days after infection to submit to HpSA test and H. felis-specific PCR. As the results, thesensitivity of the HpSA and the PCR analysis was 50.0%, 83.3% respectively. Although HpSA test isless sensitive, it could be used for rapid, cheap and easy screening assay for H. felis infection in dogand cats. We suggest that the H. pylori stool antigen kit, HpSA, is useful and effective for monitoringH. felis infection. If HpSA test would be made with H. felis antibodies in the future, its sensitivity couldbe increased. Also, PCR assay could be successfully used to detect the H. felis in stools. Applying theH. pylori stool antigen kit and PCR assay may be the recommended non-invasive strategy to identifyH. felis in dog and cats.
Abstract
The aim of the present study was to compare the non-invasive methods for the diagnosis of H. feliswith HpSA kit-based detection method and H. felis-specific PCR assay with dog’s stool samples withoutsacrifice. Male Beagle dogs (n=6) were infected with H. felis ATCC 49179 (1.0×109 CFU/dog) by intragastricinoculation two times at 3-day intervals, and the stool specimens of dogs were collected 1, 3,5, 7, 14, 21 days after infection to submit to HpSA test and H. felis-specific PCR. As the results, thesensitivity of the HpSA and the PCR analysis was 50.0%, 83.3% respectively. Although HpSA test isless sensitive, it could be used for rapid, cheap and easy screening assay for H. felis infection in dogand cats. We suggest that the H. pylori stool antigen kit, HpSA, is useful and effective for monitoringH. felis infection. If HpSA test would be made with H. felis antibodies in the future, its sensitivity couldbe increased. Also, PCR assay could be successfully used to detect the H. felis in stools. Applying theH. pylori stool antigen kit and PCR assay may be the recommended non-invasive strategy to identifyH. felis in dog and cats.
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