Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background/Aims: The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans. Methods: Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation. Results: Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively. Conclusions: The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.
Abstract
Background/Aims: The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans. Methods: Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation. Results: Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively. Conclusions: The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.
참고문헌 (23)
- Goodwin CS, "Helicobacter pylori infection", Lancet, 349, pp.265~269 (1997)
- Pelayo Correa, "Evolutionary History of the Helicobacter pylori Genome: Implications for Gastric Carcinogenesis", Gut and Liver, 6(1), pp.21~28 (2012), 거트앤리버 발행위원회
- Blaser MJ, "Ecology of Helicobacter pylori in the human stomach", J Clin Invest, 100, pp.759~762 (1997)
- Hidekazu Suzuki, "Helicobacter pylori and Gastric Mucosa-associated Lymphoid Tissue (MALT) Lymphoma: Updated Review of Clinical Outcomes and the Molecular Pathogenesis", Gut and Liver, 3(2), pp.81~87 (2009), 거트앤리버 발행위원회
- Rautelin H, "Diagnosis of Helicobacter pylori infection", Helicobacter, 8(1), pp.13~20 (2003)
- Megraud F, "Helicobacter pylori detection and antimicrobial susceptibility testing", Clin Microbiol Rev, 20, pp.280~322 (2007)
- Tsuchiya M, "Helicobacter pylori urease inhibition by rabeprazole, a proton pump inhibitor", Biol Pharm Bull, 18, pp.1053~1056 (1995)
- Varoli O, "Presence of Helicobacter pylori in gastric juice", Am J Gastroenterol, 86, pp.249~ (1991)
- Chey WD, "Practice Parameters Committee of the American College of Gastroenterology. American College of Gastroenterology guideline on the management of Helicobacter pylori infection", Am J Gastroenterol, 102, pp.1808~1825 (2007)
- Prince MI, "The CLO test in the UK: inappropriate reading and missed results", J Gastroenterol Hepatol, 11, pp.1251~1254 (1999)
- Tokunaga Y, "Modified rapid urease test for Helicobacter pylori detection in relation to an immunohistochemical stain", J Gastroenterol Hepatol, 15, pp.617~621 (2000)
- Zaitoun AM, "Histology compared with chemical testing for urease for rapid detection of Helicobacter pylori in gastric biopsy specimens", J Clin Pathol, 46, pp.684~685 (1993)
- Borromeo M, "Evaluation of “CLO-test” to detect Campylobacter pyloridis in gastric mucosa", J Clin Pathol, 40, pp.462~463 (1987)
- Cox AJ, "Stomach size and its relation to chronic peptic ulcer", AMA Arch Pathol, 54, pp.407~422 (1952)
- Hack HM, "Testing negative for Helicobacter pylori after methylene blue spraying of gastric mucosa", Gastrointest Endosc, 40, pp.397~398 (1994)
- Laine L, "Prospective comparison of commercially available rapid urease tests for the diagnosis of Helicobacter pylori", Gastrointest Endosc, 44, pp.523~526 (1996)
- Gisbert JP, "Review article: 13C-urea breath test in the diagnosis of Helicobacter pylori infection: a critical review", Aliment Pharmacol Ther, 20, pp.1001~1017 (2004)
- Makristathis A, "Two enzyme immunoassays and PCR for detection of Helicobacter pylori in stool specimens from pediatric patients before and after eradication therapy", J Clin Microbiol, 38, pp.3710~3714 (2000)
- Agha-Amiri K, "A novel immunoassay based on monoclonal antibodies for the detection of Helicobacter pylori antigens in human stool", Z Gastroenterol, 39, pp.555~560 (2001)
- Monteiro L, "Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors", J Microbiol Methods, 45, pp.89~94 (2001)
- Demirturk L, "Does N-acetyl cystein affect the sensitivity and specificity of Helicobacter pylori stool antigen test?", Helicobacter, 8, pp.120~123 (2003)
- Yakoob J, "Gastric juice for the diagnosis of H. pylori infection in patients on proton pump inhibitors", World J Gastroenterol, 14, pp.1539~1543 (2008)
- Roosendaal R, "Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR", J Clin Microbiol, 32, pp.1123~1126 (1994)