The herd-level prevalence of antibodies against Mycobacterium avium subspecies paratuberculosis in the Gyeonggi and Chungbuk of Korea, as detected by bulk tank milk ELISA
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Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease (JD) in ruminants. This is the first large scale report to estimate the herd-level prevalence of antibodies against MAP by using an ELISA to detect antibodies in bulk tank milk (BTM) samples. The samples were collected from January 2011 to November 2011, from 636 herds of the dairy farms in the Gyeonggi and Chungbuk areas of Korea. The overall apparent prevalence of MAP antibody-positive herds was 8.5%, and regional prevalence were 32/440 (7.3%) and 22/196 (11.2%) of dairy farms in the Gyeonggi and Chungbuk areas, respectively. The results did not differ significantly by region. While we have determined the prevalence rate of MAP in the Gyenoggi and Chungbuk areas in this study, there is a continuing need for well-designed studies to calculate the prevalence of MAP in dairy herds based on culture and molec-ular findings.
Abstract
Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease (JD) in ruminants. This is the first large scale report to estimate the herd-level prevalence of antibodies against MAP by using an ELISA to detect antibodies in bulk tank milk (BTM) samples. The samples were collected from January 2011 to November 2011, from 636 herds of the dairy farms in the Gyeonggi and Chungbuk areas of Korea. The overall apparent prevalence of MAP antibody-positive herds was 8.5%, and regional prevalence were 32/440 (7.3%) and 22/196 (11.2%) of dairy farms in the Gyeonggi and Chungbuk areas, respectively. The results did not differ significantly by region. While we have determined the prevalence rate of MAP in the Gyenoggi and Chungbuk areas in this study, there is a continuing need for well-designed studies to calculate the prevalence of MAP in dairy herds based on culture and molec-ular findings.
참고문헌 (11)
- Boadella M, "Serosurvey for selected pathogens in Iberian roe deer", BMC Vet Res, 6, pp.51~ (2010)
- Favila-Humara LC, "Mycobacterium avium subsp. paratuberculosis detection in individual and bulk tank milk samples from bovine herds and caprine flocks", Foodborne Pathoq Dis, 7, pp.351~355 (2010)
- Garcia-Perez AL, "Short communication: investigation of Coxiella burnetii occurrence in dairy sheep flocks by bulk-tank milk analysis and antibody level determination", J Dairy Sci, 92, pp.1581~1584 (2009)
- Grant IR, "Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows' milk from approved dairy processing establishments in the United Kingdom", Appl Environ Microbiol, 68, pp.2428~2435 (2002)
- Herthnek D, "A robust method for bacterial lysis and DNA purification to be used with real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in milk", J Microbiol Methods, 75, pp.335~340 (2008)
- Kennedy DJ, "Control of Mycobacterium avium subsp. paratuberculosis infection in agricultural species", Rev Sci Tech, 20, pp.151~179 (2001)
- Lee KW, "Seroprevalence of Mycobacterium avium subspecies paratuberculosis in cattle in Korea", Vet Rec, 16, pp.661~662 (2009)
- Mendoz JL, "Mycobacterium avium subspecies paratuberculosis and its relationship with Crohn's disease", World J Gastroenterol, 15, pp.417~422 (2009)
- Ott SL, "Herd-level economic losses associated with Johne's disease on US dairy operations", Prev Vet Med, 40, pp.179~192 (1999)
- 박용호, "Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea", Journal of Veterinary Science, 7(4), pp.349~354 (2006), 대한수의학회
- Wilson DJ, "Herd-level prevalence of Johne's disease in Utah and adjacent areas of the intermountain west as detected by a bulk-tank milk surveillance project", Journal of dairy science, 93, pp.5792~5797 (2010)