구제역바이러스 혈청형 A 검출을 위한 peptide nucleic acid (PNA)기반 multiplex real-time RT-PCR 개발
Developing peptide nucleic acid based multiplex real time RT-PCR to detect Foot-and-Mouth-Disease virus Serotype A
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
There have been a total tenth FMD outbreaks in Korea and for the first time, type O and A were de-tected simultaneously in 2017, which led to difficulties in FMD control. For the effective prevention of FMD, the importance of discrimination of serotypes became greater. Therefore, the most urgent re-quirement in case of FMD outbreak is differential diagnosis of serotypes. In this study, we developed a PNA probe-mediated multiplex real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using the peptide nucleic acid (PNA) probe, which is known to be stable to nucleotide mutation and that could specifically detect the all FMDV serotype A, FMDVA Yeoncheon strain which was oc-curred in Korea in 2017, and FMDV A viruses shown 96% similarity with FMDVA/Yeoncheon strain, at the same time. Therefore, It is believed that the newly introduced FMDVA will be effectively diag-nosed using the PNA probe multiplex RT-PCR developed in this study, and ultimately contribute to the prevention of FMD.
Abstract
There have been a total tenth FMD outbreaks in Korea and for the first time, type O and A were de-tected simultaneously in 2017, which led to difficulties in FMD control. For the effective prevention of FMD, the importance of discrimination of serotypes became greater. Therefore, the most urgent re-quirement in case of FMD outbreak is differential diagnosis of serotypes. In this study, we developed a PNA probe-mediated multiplex real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using the peptide nucleic acid (PNA) probe, which is known to be stable to nucleotide mutation and that could specifically detect the all FMDV serotype A, FMDVA Yeoncheon strain which was oc-curred in Korea in 2017, and FMDV A viruses shown 96% similarity with FMDVA/Yeoncheon strain, at the same time. Therefore, It is believed that the newly introduced FMDVA will be effectively diag-nosed using the PNA probe multiplex RT-PCR developed in this study, and ultimately contribute to the prevention of FMD.
참고문헌 (13)
- Abeyratne SAE, "The phylogeneticanalysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage", PLo S One, 13, pp.e0194077~ (2018)
- Alexandersen S, "Foot-and-mouth disease : host range and pathogenesis", Curr Top Microbio lImmunol, 288, pp.9~42 (2005)
- Grubman MJ, "Foot-and-mouth disease", Clin Microbiol Rev, 17, pp.465~493 (2004)
- Hur D, "Detection of genetic variation using dual-labeled peptide nucleicacid(PNA)probe-based melting pointanalysis", Biol Proced Online, 17, pp.14~ (2015)
- Jamal SM, "Foot-and-mouth disease : past, present and future", VetRes, 44, pp.116~ (2013)
- Jeong DJ, "Applications of Peptide Nucleic Acid(PNA)in the Biomedicine", Journal of Soonchunhyang Medical Science, 15, pp.51~58 (2009)
- Knowles NJ, "Molecular epidemiology of foot-and-mouth disease virus", Virus Res, 91, pp.65~80 (2003)
- Park JH, "Control of foot-and-mouth disease during 2010-2011 epidemic, South Korea", Emerg Infect Dis, 19, pp.655~659 (2013)
- Pooga M, "PNA oligomers as tools for specific modulation of gene expression", Biomol Eng, 17, pp.183~192 (2001)
- Reid S. M, "Primary diagnosis of Foot-and-mouth disease by reversetranscription polymerase chain reaction", J Virol Methods, 89, pp.167~176 (2000)
- Reid SM, "Development of tailored real-time RT-PCR assays for the detection and differentiation of serotype O, A and Asia-1 foot-and-mouth disease virus lineage scirculating in the Middle East", J Virol Methods, 207, pp.146~153 (2014)
- Rweyemamu M, "Epidemiological patterns of foot-and-mouth disease world wide", Transbound Emerg Dis, 55, pp.5557~5572 (2008)
- Vilaivan T, "Fluorogenic PNA probes", Beilstein J Org Chem, 14, pp.253~281 (2018)