PRRS 저항성 유전형 자돈의 생산 및 평가
Production and evaluation of PRRS resistant pigs
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Porcine reproductive and respiratory syndrome (PRRS) is economically the most important and challeng-ing disease in swine industries worldwide and caused by PRRS virus (PRRSV). Previous studies re-ported that pigs with heterozygous genotypes in the guanylate-binding proteins (GBP1 and GBP5) ex-hibited increased resistance against PRRSV infection. The present study was conducted to produce high-er numbers of the heterozygous pigs based on the PRRS resistant polymorphisms found in GBP1 (GBP1E2 and WUR) and GBP5, and evaluate the resistance of heterozygous pigs against challenge with a type 2 PRRSV (JA142) in comparison with homozygous pigs. In the challenge study, 12, 4 week-old PRRSV-negative piglets were selected based on the genotypes of the 3 polymorphisms (GBP1E2, WUR and GBP5). Among them, 8 piglets [homozygous (n=4) and heterozygous (n=4)] were challenged with JA142 and kept in the same room, and the remaining 4 piglets were kept separately as a negative control. In results, the sperms collected from the boars of GBP1E2-GG genotype produced approx-imately 28∼41% higher numbers of heterozygous piglets as compared with those from the boars of GBP1E2-AG genotype. In the challenge study, we found that heterozygous piglets showed the sig-nificantly lower levels of viremia than homozygous piglets at 14, 21 and 28 dpc. Consistently, these heterozygous piglets also exhibited significantly higher ADWG than homozygous piglets. Therefore, in the current study, selection of boars based on SNP markers could increase the production of PRRS re-sistant piglets and the PRRS resistant pigs were found to be more resistant to PRRSV infection.
Abstract
Porcine reproductive and respiratory syndrome (PRRS) is economically the most important and challeng-ing disease in swine industries worldwide and caused by PRRS virus (PRRSV). Previous studies re-ported that pigs with heterozygous genotypes in the guanylate-binding proteins (GBP1 and GBP5) ex-hibited increased resistance against PRRSV infection. The present study was conducted to produce high-er numbers of the heterozygous pigs based on the PRRS resistant polymorphisms found in GBP1 (GBP1E2 and WUR) and GBP5, and evaluate the resistance of heterozygous pigs against challenge with a type 2 PRRSV (JA142) in comparison with homozygous pigs. In the challenge study, 12, 4 week-old PRRSV-negative piglets were selected based on the genotypes of the 3 polymorphisms (GBP1E2, WUR and GBP5). Among them, 8 piglets [homozygous (n=4) and heterozygous (n=4)] were challenged with JA142 and kept in the same room, and the remaining 4 piglets were kept separately as a negative control. In results, the sperms collected from the boars of GBP1E2-GG genotype produced approx-imately 28∼41% higher numbers of heterozygous piglets as compared with those from the boars of GBP1E2-AG genotype. In the challenge study, we found that heterozygous piglets showed the sig-nificantly lower levels of viremia than homozygous piglets at 14, 21 and 28 dpc. Consistently, these heterozygous piglets also exhibited significantly higher ADWG than homozygous piglets. Therefore, in the current study, selection of boars based on SNP markers could increase the production of PRRS re-sistant piglets and the PRRS resistant pigs were found to be more resistant to PRRSV infection.
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