일반돼지와 면역 거부반응이 억제된 형질전환돼지의 혈액 성상 비교
Comparison of hematological values of conventional pigs and transgenic pigs supressed in immune rejection response
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Blood test is a useful tool in establishing medical treatment for livestock. It provides information such as disease diagnosis, treatment effects, prognostic judgment, and health status. This study compared the value of erythrocytes and leukocytes among conventional, transgenic miniature, and transgenic conven-tional pigs aged six months to 24 months. Further, it analyzed the aspects of hematological value changes according to the pigs’ ages. As a result, the number of red blood cells (RBC), which include hemoglobin, and hematocrit, and the number of white blood cells (WBC), which include neutrophils, and lymphocyte, were high among transgenic miniature pigs, compared with the conventional and trans-genic conventional pigs. Conventional pigs showed similar values of RBC and WBC regardless of transgenesis. In comparing their age, the RBC decreased as the age increased compared with the pigs among all the three groups aged of 6∼12 months. On the other hand, WBC and neutrophils showed no significant difference regardless of different ages among all the three groups. However, various counts in RBC and WBC were mostly found to be higher in each age in transgenic miniature pigs than in conventional and transgenic conventional pigs. The results of this study show that the values of RBC and WBC were generally higher in transgenic miniature pigs than in conventional and transgenic con-ventional pigs. Based on this research, hematological values can be widely used in diagnosing diseases or checking the health status of transgenic pigs that are used as disease models, organ transplant source and alike.
Abstract
Blood test is a useful tool in establishing medical treatment for livestock. It provides information such as disease diagnosis, treatment effects, prognostic judgment, and health status. This study compared the value of erythrocytes and leukocytes among conventional, transgenic miniature, and transgenic conven-tional pigs aged six months to 24 months. Further, it analyzed the aspects of hematological value changes according to the pigs’ ages. As a result, the number of red blood cells (RBC), which include hemoglobin, and hematocrit, and the number of white blood cells (WBC), which include neutrophils, and lymphocyte, were high among transgenic miniature pigs, compared with the conventional and trans-genic conventional pigs. Conventional pigs showed similar values of RBC and WBC regardless of transgenesis. In comparing their age, the RBC decreased as the age increased compared with the pigs among all the three groups aged of 6∼12 months. On the other hand, WBC and neutrophils showed no significant difference regardless of different ages among all the three groups. However, various counts in RBC and WBC were mostly found to be higher in each age in transgenic miniature pigs than in conventional and transgenic conventional pigs. The results of this study show that the values of RBC and WBC were generally higher in transgenic miniature pigs than in conventional and transgenic con-ventional pigs. Based on this research, hematological values can be widely used in diagnosing diseases or checking the health status of transgenic pigs that are used as disease models, organ transplant source and alike.
참고문헌 (22)
- Allan JS, "Morphometric analyses to predict appropriate donor size for swine-tohuman cardiac xenotransplantation", Transplant Proc, 31, pp.975~977 (1999)
- Burks MF, "Age and sex related changes of hematologic parameters in Sinclair(S-1)miniature swine", Growth, 41(1), pp.51~62 (1977)
- Chen J, "Symptoms of systemic lupus erythematosus are diagnosed in leptin transgenic pigs", PLoS Biol, 16(8), pp.e2005354~ (2018)
- Chen L, "Long-term toxicity study on genetically modified corn with cryAc gene in a Wuzhishan miniature pig model", J Sci Food Agric, 96, pp.4207~4214 (2016)
- Changyong Choe, "Hematological analysis of the Korean native cattle (Hanwoo) according to the period and method of grazing", 한국동물위생학회지, 41(3), pp.191~196 (2018), 한국동물위생학회
- Cozzi E, "The generation of transgenic pigs as potential orgna donor for humans", Nat Med, 1(9), pp.964~966 (1995)
- Dubreuil P, "Hematological and biochemical changes following an acute stress in control and somatostatin-immunized pigs", Can J Anim Sci, 73, pp.241~252 (1993)
- Faustini M, "Haematology and plasma biochemistry of Stamboek pre-pubertal gilts in Italy : reference values", J Vet Med A Physiol Pathol Clin Med, 47(9), pp.525~532 (2000)
- Fisher DD, "Environmental and genetic effects on hematologic characteristics of beef cows", Am J Vet Res, 41, pp.1533~1536 (1980)
- Friendship RM, "Hematology and biochemistry reference values for Ontario swine", Can J Comp Med, 48, pp.390~393 (1984)
- 정주현, "Hematology and Serum Chemistry Values of Growing Miniature Pigs", 생명과학회지, 18(8), pp.1169~1172 (2008), 한국생명과학회
- 김현, "성장 단계별 희소 한우(칡소)의 혈액학적 분석", 한국동물생명공학회지, 29(3), pp.313~319 (2014), 사단법인 한국동물생명공학회
- 김기현, "자동혈구분석기 Hemavet 950FS를 이용한 돼지 혈액 내 혈구분석의 유효시간 평가", 한국산학기술학회논문지, 18(1), pp.194~201 (2017), 한국산학기술학회[DOI]
- Klem TB, "Hematologic and biochemical reference intervals for Norwegian crossbreed grower pigs", Vet Clin Pathol, 39(2), pp.221~226 (2010)
- Lee YG, "Changes of hematological composition during pregnancy in the next generation of hEPO transgenic pigs", Korean J Animal Reprod Proceeding, pp.0302~ (2003)
- Liu Q, "Effects of 60-week feeding diet containing Bt rice expressing the Cry1Ab protein on the offspring of inbred Wuzhishan pigs fed the same diet", J Agric Food Chem, 65, pp.10300~10309 (2017)
- Lumsden JH, "On establishing reference values", Can J Comp Med, 42, pp.293~301 (1978)
- Meurens F, "The pig; a model for human infectious diseases", Trend Microbiol, 20(1), pp.50~57 (2012)
- Mills CF, "The detection of trace element deficiency and excess in man and farm animals", Proc Nutr Soc, 33, pp.267~274 (1974)
- Terada Y, "Resistibility to Theileria sergenti infection in Holstein and Japanese Black cattle", J Vet Med Sci, 57, pp.1003~1006 (1995)
- Tucker A, "The production of transgenic pigs for potential use in clinical xenotransplantation : baseline clinical pathology and organ size studies", Xenotransplantation, 2002(9), pp.203~208 (2002)
- Ye Y, "The pig as a potential organ donor for man. A study of potentially transferable disease from donor pig to recipient man", Transplantation, 57(5), pp.694~703 (1994)