원유중 Ivermectin 구충제의 잔류실태 조사
Examination of ivermectin residues in raw milk after skin administration
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Abstract
This study was conducted two kinds of aims: 1) to modify the analytical methods(conditions) by high performance liquid chromatography-fluorescence detector for the detection of residual ivermectin in raw milk, 2) to provide basic information for the evaluation of standard of the residual ivermectin in raw milk.It could be considerable that negative ion spectra can be better method in the LC/MS analysis for the detection of residues. Characteristic daughter ions were observed in negative ion spectra, however, linear line was not formed in positive ion one.Three Holstein cows (500±10kg) were applied to commercial ointment of ivermectin just one time at the first day of test, and residues in raw milk were examined for 20day after administration. The limit of detection(LOD) was 0.65ng(n=5) by HPLC/FLD, and recovery rates were 87.85%~99.47%. The peak was observed at the 4th day, and residues lasted to the end. Thus ivermectin was prohibited when lactating.
참고문헌 (10)
- Codex Alimentarius Commission, "Maximum residues limits for veteri?nary drug in foods", pp.1~25 (2005), update as at the 28th session of the Codex Alimen?tarius Commission
- 식품의약품안전처, "동물용의약품의 잔류허용기준", pp.1~7 (2002), 식품의약품안전처
- Pollmeier M, "High-performance liquid chro?matographic assay for the determi?nation of a semisynthetic avermectin analog (eprinomectin) in bovine milk at parts per billion levels", 772, pp.99~105 (2002)
- Danaher M, "Validation and robustness testing of a HPLC method for the determi?nation of avermectins and moxidectin in animal liver samples using an alu?mina column clean-up", 125, pp.1741~1744 (2000)
- Stout SJ, "Confirmation of moxi?dectin residues in cattle fat by liquid chromatography", mass spectrometry, 2000, pp.83(6), 1446~1450
- Yoshii K, "Liquid chromatographic deter?mination of emamectin, milbemectin, ivermectin and abamectin in crops and confirmation by liquid chromato?graphy?mass spectrometry", 895, pp.75~85 (2000)
- Howells L, "residue analysis of avermectins and moxidectin by ion?trap LC?MSn", 126, pp.155~160 (2001)
- Durden DA, "Positive and nega?tive electrospray LC-MS-MS methods for quantitation of the antiparasitic endectocide drugs, abamectin, doramec?tin, emamectin, eprinomectin, iver?mectin, moxidectin and selamectin in milk", 850(mectin), pp.134~146 (2007)
- Sheridan R, "Determination of abamectin, doramec?tin, emamectin, eprinomectin, iver?mectin, and moxidectin in milk by liquid chromatography electrospray tandem mass specrometry", 89, pp.1088~1094 (2006)
- Imperiale F, "Comparative depletion of ivermectin and moxidectin milk resi?dues in dairy sheep after oral and subcutaneous administration", 71, pp.427~433 (2004)