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Salmonella enterica serovars Gallinarum과 S Pullorum의 감별을 위한 2가지 진단법: allele-specific real-time PCR과 3'-tailed PCR의 비교

Comparison of two diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR to discriminate between Salmonella enterica serovars Gallinarum and S Pullorum

김태중|이재일|이세미|서자영
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2008.12|한국동물위생학회지|제31권 제4호|pp. 485-492

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참고문헌

주제분류수의학

초록

Abstract

Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms (237th and 598th). In both methods, 237th allele was found to be a good target for differential diagnosis, while 598th allele produced some non-specific reactions.

참고문헌 (27)

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