Monoclonal antibody-based enzyme immuno-slide assay (EISA) in the rapid diagnosis of Peste des petits ruminants of goats
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique., respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.
Abstract
Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique., respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.
참고문헌 (18)
- Anderson J., "The use of monoclonal antibodies in competitive ELISA for the detection of antibodies to rinderpest and peste des petits ruminants viruses: the sero-monitoring of rinderpest throughout Africa. Phase one. Proceedings of a final research coordination meeting of the FAO-IAEA-SIDA-OAU-IBAR-PARC Co-ordinated Research Programme. Held in Binger- bille, Cote d’Ivoire, 19-23 Nov 1990", IAEATECHDOC, 623, pp.45~53 (1990)
- Dhar P, "Recent epidemiology of peste des petits ruminants virus (PPRV)", Vet Microbiol, 88(2), pp.153~159 (2002)
- Debasis J, "Incidence of an explosive outbreak of peste des petits ruminants (PPR) in Black Bengal goats in Bankura district of West Bengal", Indian Vet J, 79(7), pp.739~740 (2002)
- Debnath NC, "Peste des petits ruminants (PPR); An overview", Proceedings of the BSVER symposium on eradication of rinderpest and related disease, pp.9~13 (1995)
- Diallo A, "Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones", J Virol Method, 23(2), pp.127~136 (1989)
- Diallo A, "Comparison of proteins induced in cells infected with rinderpest and peste des petits ruminants viruses", J Gen Virol, 68, pp.2033~2038 (1987)
- Islam MR, "An out break of Peste des petits ruminants in Black Bengal goats in Mymensingh, Bangladesh", Bangladesh, 18(1), pp.14~19 (2001)
- Kumar A, "PPR outbreaks in goats: epidemiological and therapeutic studies", Indian J Anim Sci, 71(9), pp.815~818 (2001)
- Libeau G, "Comparison of rinderpest and PPR viruses using antinucleoprotein monoclonal antibodies", Vet Microbiol, 25(1), pp.1~16 (1990)
- Libeau G, "Rapid differential diagnosis of Rinderpest and PPR using an immunocapture ELISA", Vet Rec, 134(12), pp.300~304 (1994)
- Mondal AK, "Report on epizootological and clinico-pathological observationon peste des petits ruminants (PPR) in West Bengal", Indian J Animal Health Bull, 64, pp.261~ (1995)
- Pawaiya RVS, "Pathology and distribution of antigen in goats naturally infected with peste des petits ruminants virus", Indian J Anim Sci, 74, pp.35~40 (2004)
- Plowright W, "Rinderpest Virus", pp.25~ (1968), Springer Verlag
- Shankar H, "Occurrence of peste des petits ruminats like disease in small ruminants in Uttar Pradesh", Indian J Animal Sci, 68, pp.38~40 (1998)
- Sil BK, "Development of Mab-based Enzyme immuno slide Assay (EISA) for the rapid detection and accurate field diagnosis of peste des petits ruminants virus", Bangladesh J Lives Res, 7, pp.48~63 (2001)
- Taylor WP, "Serological studies with the virus of PPR in Nigeria", Res Vet Sci, 26, pp.236~ (1979)
- Toplu N, "Characteristic and non-characteristic pathological findings in peste des petits ruminants (PPR) of sheep in the Ege District of Turkey", J Com Pathol, 131, pp.135~141 (2004)
- Wamwayi HM, "Confirmation of riderpest in experimentally and naturally infected cattle using microtitre techniques", Trop Animal Health Product, 23(1), pp.17~21 (1991)