돼지고기 제품 내 닭고기 검출을 위한 TaqManⓇ real-time PCR의 적용
Application of the TaqManⓇ real-time PCR assay for the detection of chicken (Gallus gallus) meat in pork products
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초록
Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqManⓇ quantitative real-time polymerase chain reaction (PCR) as-say was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient (R2) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not con-taminated with chicken residuals. Only twelve pork products of one of the manufacturers were in ac-cordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consum-ers' trust.
Abstract
Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqManⓇ quantitative real-time polymerase chain reaction (PCR) as-say was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient (R2) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not con-taminated with chicken residuals. Only twelve pork products of one of the manufacturers were in ac-cordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consum-ers' trust.
참고문헌 (22)
- Ballin NZ, "Authentication of meat and meat products", Meat Science, 86, pp.577~587 (2010)
- Ballin NZ, "Species determination - Can we detect and quantify meat adulteration?", Meat Science, 83, pp.165~174 (2009)
- Bottero MT, "Animal species identification in food products: evolution of biomolecular methods", Vet J, 190, pp.34~38 (2011)
- Buntjer JB, "Species identification by oligonucleotide hybridisation: the influence of processing of meat products", J Sci Food Agric, 79, pp.53~57 (1999)
- Cammà C, "Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures", Food Control, 23, pp.400~404 (2012)
- Di Pinto A, "Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources", Food Control, 16, pp.391~394 (2005)
- Dooley JJ, "Detection of meat species using TaqMan real-time PCR assays", Meat Science, 68, pp.431~438 (2004)
- Fajardo V, "A review of current PCR-based methodologies for the authentication of meats from game animal species", Trends in Food Science & Technology, 21, pp.408~421 (2010)
- Jonker KM, "Species identification in meat products using real-time PCR", Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 25, pp.527~533 (2008)
- Ghovvati S, "Fraud identification in industrial meat products by multiplex PCR assay", Food Control, 20, pp.696~699 (2009)
- 고바라다, "식육감별을 위한 미토콘드리아 12S rRNA와 16S rRNA 유전자의 종 특이적 multiplex PCR 기법 개발", 한국가축위생학회지, 34(4), pp.417~428 (2011), 한국가축위생학회
- 고바라다, "소, 돼지, 가금육류의 신속한 동정을 위한 TaqManⓇprobe를 이용한 real-time PCR 개발", 한국가축위생학회지, 35(3), pp.215~222 (2012), 한국가축위생학회
- Laube I, "Methods for the detection of beef and pork in foods using real-time polymerase chain reaction", Food Sci Technol Int, 38, pp.111~118 (2003)
- Laube I, "Quantitative determination of commercially relevant species in foods by real-time PCR", Food Sci Technol Int, 42, pp.336~341 (2007)
- Laube I, "Development and design of a ‘ready-to-use’ reaction plate for a PCR-based simultaneous detection of animal species used in foods", Food Sci Technol Int, 42, pp.9~17 (2007)
- López-Andreo M, "Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction", Anal Biochem, 339, pp.73~82 (2005)
- Lopparelli RM, "Real-time TaqMan polymerase chain reaction detection and quantification of cow DNA in pure water buffalo mozzarella cheese: method validation and its application on commercial samples", J Agric Food Chem, 55, pp.3429~3434 (2007)
- Meyer R, "Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food", J AOAC Int, 78, pp.1542~1551 (1995)
- Montiel-Sosa JF, "Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA", J Agric Food Chem, 48, pp.2829~2832 (2000)
- Prado M, "Novel approach for interlaboratory transfer of real-time PCR methods: detecting bovine meat and bone meal in feed", Anal Bioanal Chem, 39, pp.1423~1431 (2009)
- Rojas M, "Application of a real-time PCR assay for the detection of ostrich (Struthio camelus) mislabelling in meat products from the retail market", Food Control, 22, pp.523~531 (2011)
- Tanabe S, "A real-time quantitative PCR detection method for pork, chicken, beef, mutton, and horseflesh in foods", Biosci Biotechnol Biochem, 71, pp.3131~3135 (2007)