본문 바로가기
KERD
논문KCI 등재

Effects of bone morphogenetic protein-2 and activated platelet-rich plasma on canine chondrocytes in monolayer cultures

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수의학

초록

This study evaluated the effects of bone morphogenetic protein-2 (BMP-2) and platelet-rich plasma (PRP) on canine chondrocytes under inflammatory conditions. Four groups were tested: control, BMP, PRP, and PRP+BMP. Inflammatory conditions were induced using lipopolysaccharide. Realtime polymerase chain reaction (PCR) was performed to measure the expression of COL1A1, COL2A1, interleukin (IL)-1β, and osteocalcin, and a cell proliferation assay was conducted. Cell proliferation assays showed significant increases in both PRP and PRP+BMP groups, with no significant difference between them. In the real-time PCR, the BMP group showed no change in COL1A1 expression compared to the control group, but a significant increase in COL2A1 expression was observed. In contrast, the PRP and PRP+BMP groups showed significant decreases in both COL1A1 and COL2A1 expression. IL-1β expression was significantly decreased in the PRP and PRP+BMP groups, but no differences were observed in the BMP group compared to the control group. Regarding osteocalcin mRNA expression, no significant difference was observed between the control and BMP groups, but significant decreases were identified in the PRP and PRP+PRP groups. In conclusion, PRP enhanced cell proliferation and exhibited anti-inflammatory effects on chondrocytes, while BMP-2 increased COL2A1 gene expression. Interestingly, BMP-2 did not increase osteocalcin gene expression, which is associated with the osteoblast differentiation of stem cells, and the PRP groups exhibited inhibited osteocalcin gene expression under inflammatory conditions.

Abstract

This study evaluated the effects of bone morphogenetic protein-2 (BMP-2) and platelet-rich plasma (PRP) on canine chondrocytes under inflammatory conditions. Four groups were tested: control, BMP, PRP, and PRP+BMP. Inflammatory conditions were induced using lipopolysaccharide. Realtime polymerase chain reaction (PCR) was performed to measure the expression of COL1A1, COL2A1, interleukin (IL)-1β, and osteocalcin, and a cell proliferation assay was conducted. Cell proliferation assays showed significant increases in both PRP and PRP+BMP groups, with no significant difference between them. In the real-time PCR, the BMP group showed no change in COL1A1 expression compared to the control group, but a significant increase in COL2A1 expression was observed. In contrast, the PRP and PRP+BMP groups showed significant decreases in both COL1A1 and COL2A1 expression. IL-1β expression was significantly decreased in the PRP and PRP+BMP groups, but no differences were observed in the BMP group compared to the control group. Regarding osteocalcin mRNA expression, no significant difference was observed between the control and BMP groups, but significant decreases were identified in the PRP and PRP+PRP groups. In conclusion, PRP enhanced cell proliferation and exhibited anti-inflammatory effects on chondrocytes, while BMP-2 increased COL2A1 gene expression. Interestingly, BMP-2 did not increase osteocalcin gene expression, which is associated with the osteoblast differentiation of stem cells, and the PRP groups exhibited inhibited osteocalcin gene expression under inflammatory conditions.

참고문헌 (36)

  1. Akeda K, "Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis", Osteoarthritis Cartilage, 14(12), pp.1272~1280 (2006)
  2. Alvarez-Soria MA, "Long-term NSAID treatment directly decreases COX-2 and mPGES-1 production in the articular cartilage of patients with osteoarthritis", Osteoarthritis Cartilage, 16(12), pp.1484~1493 (2008)
  3. Arnoczky SP, "What Is Platelet-Rich Plasma?", Oper Tech Sports Med, 19(3), pp.142~148 (2011)
  4. Asjid R, "Platelet-rich plasma-induced inhibition of chondrocyte apoptosis directly affects cartilage thickness in osteoarthritis", Cureus, 11(11), pp.e6050~ (2019)
  5. Bendinelli P, "Molecular basis of anti-inflammatory action of platelet-rich plasma on human chondrocytes : mechanisms of NF-κB inhibition via HGF", J Cell Physiol, 225(3), pp.757~766 (2010)
  6. Blaney Davidson EN, "Inducible chondrocyte-specific overexpression of BMP2 in young mice results in severe aggravation of osteophyte formation in experimental OA without altering cartilage damage", Ann Rheum Dis, 74, pp.1257~1264 (2015)
  7. Chen AL, "Expression of bone morphogenetic proteins, receptors, and tissue inhibitors in human fetal, adult, and osteoarthritic articular cartilage", J Orthop Res, 22, pp.1188~1192 (2004)
  8. De Ceuninck F, "Culture of chondrocytes in alginate beads", Methods Mol Med, 100, pp.15~22 (2004)
  9. Fukui N, "Stimulation of BMP-2 expression by proinflammatory cytokines IL-1 and TNF-α in normal and osteoarthritic chondrocytes", JBJS, 85, pp.59~66 (2003)
  10. Gründer T, "Bone morphogenetic protein(BMP)-2 enhances the expression of type II collagen and aggrecan in chondrocytes embedded in alginate beads", Osteoarthritis Cartilage, 12(7), pp.559~567 (2004)
  11. Hardie EM, "Radiographic evidence of degenerative joint disease in geriatric cats : 100 cases(1994-1997)", J Am Vet Med Assoc, 220(5), pp.628~632 (2002)
  12. Harirforoosh S, "Adverse effects of nonsteroidal antiinflammatory drugs : an update of gastrointestinal, cardiovascular and renal complications", J Pharm Pharm Sci, 16(5), pp.821~847 (2013)
  13. Hills RL, "Bone morphogenetic protein 9 is a potent anabolic factor for juvenile bovine cartilage, but not adult cartilage", J Orthop Res, 23, pp.611~617 (2005)
  14. Hunter DJ, "Pharmacologic therapy for osteoarthritis – the era of disease modification", Nat Rev Rheumatol, 7, pp.13~22 (2011)
  15. Johnston SA, "Osteoarthritis : Joint anatomy, physiology, and pathobiology", Vet Clin North Am Small Anim Pract, 27, pp.699~ (1997)
  16. Kim D, "Platelet-rich plasma enhances proliferation and migration and inhibits inflammatory processes in canine chondrocytes", J Vet Clinics, 36, pp.200~206 (2019)
  17. Kim H, "Epigenetic regulation of chondrocyte catabolism and anabolism in osteoarthritis", Mol Cells, 38(8), pp.677~684 (2015)
  18. Ku JH, "Correlation of synovial fluid leptin concentrations with the severity of osteoarthritis", Clin Rheumatol, 28(12), pp.1431~1435 (2009)
  19. Ma B, "Gene expression profiling of dedifferentiated human articular chondrocytes in monolayer culture", Osteoarthritis Cartilage, 21(4), pp.599~603 (2013)
  20. Man GS, "Osteoarthritis pathogenesis-a complex process that involves the entire joint", J Med Life, 7(1), pp.37~41 (2014)
  21. Medvedeva EV, "Repair of damaged articular cartilage : Current approaches and future directions", Int J Mol Sci, 19, pp.2366~ (2018)
  22. Miosge N, "Expression of collagen type I and type II in consecutive stages of human osteoarthritis", Histochem Cell Biol, 122(3), pp.229~236 (2004)
  23. Moussa M, "Platelet rich plasma(PRP)induces chondroprotection via increasing autophagy, antiinflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage", Exp Cell Res, 352(1), pp.146~156 (2017)
  24. Nakase T, "Localization of bone morphogenetic protein-2 in human osteoarthritic cartilage and osteophyte", Osteoarthr Cartil, 11, pp.278~284 (2003)
  25. Noël D, "Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells", Stem Cells, 22(1), pp.74~85 (2004)
  26. Papathanasiou I, "Bone morphogenetic protein-2-induced Wnt", β-catenin signaling pathway activation through enhanced low-density-lipoprotein receptor-related protein 5 catabolic activity contributes to hypertrophy in osteoarthritic chondrocytes, 2012, pp.14, R82~
  27. Pregizer SK, "Dynamics and cellular localization of Bmp2, Bmp4, and Noggin transcription in the postnatal mouse skeleton", J Bone Miner Res, 30, pp.64~70 (2015)
  28. Pullig O, "Chondrocyte differentiation in human osteoarthritis : expression of osteocalcin in normal and osteoarthritic cartilage and bone", Calcif Tissue Int, 67(3), pp.230~240 (2000)
  29. Ripmeester EGJ, "Recent insights into the contribution of the changing hypertrophic chondrocyte phenotype in the development and progression of osteoarthritis", Front Bioeng Biotechnol, 19(6), pp.18~ (2018)
  30. Salazar VS, "BMP signalling in skeletal development, disease and repair", Nat Rev Endocrinol, 12, pp.203~ (2016)
  31. Singh P, "Phenotypic instability of chondrocytes in osteoarthritis : On a path to hypertrophy", Ann N Y Acad Sci, 1442, pp.17~34 (2019)
  32. Thielen NGM, "TGFβ", BMP signaling pathway in cartilage homeostasis, 2019, pp.8(9), 969~
  33. van Caam A, "Expression of TGFbeta-family signalling components in ageing cartilage : Age-related loss of TGFbeta and BMP receptors", Osteoarthr Cartil, 24(7), pp.1235~1245 (2016)
  34. Van der Kraan P, "Bone morphogenetic proteins and articular cartilage : To serve and protect or a wolf in sheep clothing’s?", Osteoarthr Cartil, 18, pp.735~741 (2010)
  35. Zhang J, "HGF mediates the anti-inflammatory effects of PRP on injured tendons", PLoS One, 8(6), pp.e67303~ (2013)
  36. Zheng L, "The role of metabolism in chondrocyte dysfunction and the progression of osteoarthritis", Ageing Res Rev, 66, pp.101249~ (2021)

관련 논문AI추천

Effects of hyaluronic acid and activated platelet-rich plasma in canine chondrocytesJang Min, Kim Dongwoo, Kim Jiyun, Lee Won-Jae, Yun Sung-Ho한국동물위생학회 · 한국동물위생학회지2025 · 제48권 제3호The Effects of Intra-Articular Platelet-Rich Plasma Injection According to the Severity of Collagenase-Induced Knee Osteoarthritis in a Rabbit Model권동락, 박기영, Sung-uk Lee대한재활의학회 · Annals of Rehabilitation Medicine2012 · 제36권 제4호Individual Variation in Growth Factor Concentrations in Platelet-rich Plasma and Its Influence on Human Mesenchymal Stem Cells박소영, 성민철, 송경은, 송인환, 안면환, 조희순대한진단검사의학회 · Annals of Laboratory Medicine2011 · 제31권 제3호Bone Morphogenetic Protein Receptor in the Osteogenic Differentiation of Rat Bone Marrow Stromal CellsAnxun Wang, Shihu Sheng, Xueqiang Ding, Zhaoyou Yao연세대학교의과대학 · Yonsei Medical Journal2010 · 제51권 제5호Effects of Serial Passage on the Characteristics and Chondrogenic Differentiation of Canine Umbilical Cord Matrix Derived Mesenchymal Stem Cells이경우, 이금실, 이훈택, H.W. Kang, J.-Y. Song, K.B. KO, S.-H. Cha아세아·태평양축산학회 · Animal Bioscience2013 · 제26권 제4호Therapeutic Effects of Mesenchymal Stem Cells and Hyaluronic Acid Injection on Osteochondral Defects in Rabbits’ Knees왕립, 김성수, 이규열, 이명진, Hyo Jong Kim, Min Soo Kang대한슬관절학회 · Knee Surgery and Related Research2012 · 제24권 제3호

이 간행물의 다른 논문 (14건)