The Effects of Intra-Articular Platelet-Rich Plasma Injection According to the Severity of Collagenase-Induced Knee Osteoarthritis in a Rabbit Model
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Objective To investigate whether the cartilage regenerative effects of intra-aricular platelet-rich plasma (PRP) are different, according to the severity of osteoarthritis (OA), in a collagenase-induced knee OA rabbit model.Method New Zealand white rabbits (N=21) were randomly divided into three groups. Three different doses (0.25 mg, group 1; 0.5 mg, group 2; and 1.0 mg, group 3) of collagenase were injected twice into both knees of each group under an ultrasound guidance. The mean platelet concentration of the PRP fraction was 2,664±970×103/μl and was enriched 8.2-times, compared with the whole blood. PRP (0.3 ml) was injected into the left knee and saline (0.3 ml) into the right knee at 4 weeks, and macroscopic and histological scores of both injected knees were evaluated at 9 weeks after the first collagenase injection.Results Macroscopic and histological scores of group 3 were significantly higher than those of group 1 and 2 (p<0.05). Macroscopic and histological scores of the PRP-injected knees were significantly lower than those of the saline-injected knees, in all groups (p<0.05). Differences of gross morphologic and histologic scores between sa-line- and PRP-injected knees in group 3 were significantly higher than those in group 1 and 2 (p<0.05).Conclusion Intra-articular PRP injection influences cartilage regeneration in all severities of rabbit knee OA, and the cartilage regenerative power of PRP injection in moderate knee OA was greater than that in mild or very mild OA. A large preclinical trial is needed to establish the validity of our study.
Abstract
Objective To investigate whether the cartilage regenerative effects of intra-aricular platelet-rich plasma (PRP) are different, according to the severity of osteoarthritis (OA), in a collagenase-induced knee OA rabbit model.Method New Zealand white rabbits (N=21) were randomly divided into three groups. Three different doses (0.25 mg, group 1; 0.5 mg, group 2; and 1.0 mg, group 3) of collagenase were injected twice into both knees of each group under an ultrasound guidance. The mean platelet concentration of the PRP fraction was 2,664±970×103/μl and was enriched 8.2-times, compared with the whole blood. PRP (0.3 ml) was injected into the left knee and saline (0.3 ml) into the right knee at 4 weeks, and macroscopic and histological scores of both injected knees were evaluated at 9 weeks after the first collagenase injection.Results Macroscopic and histological scores of group 3 were significantly higher than those of group 1 and 2 (p<0.05). Macroscopic and histological scores of the PRP-injected knees were significantly lower than those of the saline-injected knees, in all groups (p<0.05). Differences of gross morphologic and histologic scores between sa-line- and PRP-injected knees in group 3 were significantly higher than those in group 1 and 2 (p<0.05).Conclusion Intra-articular PRP injection influences cartilage regeneration in all severities of rabbit knee OA, and the cartilage regenerative power of PRP injection in moderate knee OA was greater than that in mild or very mild OA. A large preclinical trial is needed to establish the validity of our study.
참고문헌 (22)
- Wearing SC, "Musculoskeletal disorders associated with obesity: a biomechanical perspective", Obes Rev, 7, pp.239~250 (2006)
- Hochberg MC, "Guidelines for the medical management of osteoar thritis. Part II. Osteoarthritis of the knee. American College of Rheumatology", Arthritis Rheum, 38, pp.1541~1546 (1995)
- Goldspink DF, "Influence of pituitary growth hormone on DNA synthesis in rat tissues", Am J Physiol, 228, pp.302~309 (1975)
- Altman R, "Design and conduct of clinical trials in patients with osteoarthritis: recommendations from a task force of the Osteoarthritis Research Society. Results from a workshop", Osteoarthritis Cartilage, 4, pp.217~243 (1996)
- Namiki O, "Therapeutic effect of intra-articular injection of high molecular weight hyaluronic acid on osteoarthritis of the knee", Int J Clin Pharmacol Ther Toxicol, 20, pp.501~507 (1982)
- Anitua E, "New insights into and novel applications for platelet-rich fibrin therapies", Trends Biotechnol, 24, pp.227~234 (2006)
- Sampson S, "Platelet rich plasma injection grafts for musculoskeletal injuries: a review", Curr Rev Musculoskelet Med, 1, pp.165~174 (2008)
- Sun Y, "The regenerative effect of platelet-rich plasma on healing in large osteochondral defects", Int Orthop, 34, pp.589~597 (2010)
- Schmidt MB, "A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair", Osteoarthritis Cartilage, 14, pp.403~412 (2006)
- Kikuchi T, "Intra-articular injection of collagenase induces experimental osteoarthritis in mature rabbits", Osteoarthritis Cartilage, 6, pp.177~186 (1998)
- 김상범, "Additive Effects of Intra-articular Injection of Growth Hormone and Hyaluronic Acid in Rabbit Model of Collagenase-induced Osteoarthritis", Journal of Korean Medical Science, 25(5), pp.776~780 (2010), 대한의학회
- Huh JE, "Therapeutic effect of Siegesbeckia pubescens on cartilage protection in a rabbit collagenase-induced model of osteoarthritis", J Pharmacol Sci, 107, pp.317~328 (2008)
- Yoshimi T, "Effects of high-molecular- weight sodium hyaluronate on experimental osteoarthrosis induced by the resection of rabbit anterior cruciate ligament", Clin Orthop Relat Res, 298, pp.296~304 (1994)
- Mankin HJ, "Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data", J Bone Joint Surg Am, 53, pp.523~537 (1971)
- Qi YY, "Local delivery of autologous platelet in collagen matrix simulated in situ articular cartilage repair", Cell Transplant, 18, pp.1161~1169 (2009)
- Anitua E, "Platelet-released growth factors enhance the secretion of hyaluronic acid and induce hepatocyte growth factor production by synovial fibroblasts from arthritic patients", Rheumatology, 46, pp.1769~1772 (2007)
- Akeda K, "Plateletrich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis", Osteoarthritis Cartilage, 14, pp.1272~1280 (2006)
- Marx RE, "Platelet-rich plasma: evidence to support its use", J Oral Maxillofac Surg, 62, pp.489~496 (2004)
- Drengk A, "Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells", Cells Tissues Organs, 189, pp.317~326 (2009)
- Li M, "Assessment study on a set of platelet-rich plasma preparation", Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 25, pp.112~116 (2011)
- Fox JG, "Laboratory animal medicine, 2nd ed" (2002), Academic Press
- Cunnington J, "A randomized, double-blind, controlled study of ultrasound-guided corticosteroid injection into the joint of patients with inflammatory arthritis", Arthritis Rheum, 62, pp.1862~1869 (2010)