HPLC-ELSD법에 의한 길경의 platycodin D 정량분석
Quantitative Analysis of Platycodin D from Platycodon grandiflorum by HPLC-ELSD
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Abstract
Platycodin D was isolated from n-butanol extract of Platycodi radix(Platycodon grandiflorum, and indentified by the spectroscopic analysis using H and C NMR techniques. A new method of analysis of system with YMC-Pack ODS-AM(250 X 4.6mm) column and 30% acetonitrile as a mobile phase. Evaporative light scattering detector was used as detector. The kinds of extraction solvents and methods were examined to determine the efficient extraction condition and HPLC analysis was carried out to establish the optimum drying condition for the root of Platycodon grandiflorum. The contents of platycodin D was highest as 0.083% when platycodon roots were dried at 60℃ using dry oven.