RAPD에 의한 천문동 수집종의 유연관계 분석

Abstract

To analyze the genetic relationships among 23 accessions of Asparagus cochinensis Lour, random amplified polymorphic DNA(RAPD) analysis was performed suing artificially synthesized 10 primers. The range of polymorphism was 42.9~91.7% with an average of 72.9% in 85 randomly an specifically amplified DNA fragments. On the basis of similarity coefficient analysis by unweighed pairgroup method, arithmetic average method(UPGMA), 23 accessions of Asparagus cochinensis Lour could be classified into 6 groups at the similarity coefficient value of 0.82. Group Ⅰcontained 5 accessions Group Ⅱ contained 6 accessions, Group Ⅲ contained 6 accessions, Group Ⅳ contained 2 accessions, Group Ⅴ contained 2 accessions and Group Ⅵ contained 2 accessions. The range of total genetic similarity coefficient value of 23 accessions of Asparagus cochinchinensis Lour was 0.47~0.92 and average value was 0.76. To obtain more exact data from PCR, we also tried to develope enhanced RAPD techniques using Bovine Lacto Transfer Technique Optimizer(BLOTTO). In RAPD analysis of Asparagus cochinensis Lour we could obtain better RAPD results by adding BLOTTO at a final concentration of 1%.