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논문

디기탈리스의 잎 절편으로부터 신초의 재분화

Shoot Regeneration from leaf explants of digitalis purpurea L.

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Abstract

Digitalis purpurea L. is a medicinal herb and have been used to congestive heart failure, mycocardial infarction, edema, angina etc. A protocol has been developed for in vitro propagation of adventitious shoot buds directly from leaf segments of D. purpurea Leaf explants of D. purpurea directly formed shoot buds when cultured on a MS medium supplemented with 2 ㎖/ℓ BA and 0.1 ㎖/ℓ IAA for 5 weeks. Adventitious shoots were multiplied by subculturing on the B5 medium and shoot elongation was developed by subculturing on the WPM medium. Root formation from the shoot regenerated was achieved on MS basal medium containing 1㎎ / IBA.

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