Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban
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An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitroplantlet on MS basal medium controled with different plant growth regulators (NAA, 2,4-D, IAA kinetin, and BA). Best resultsthat 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formationof adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regeneratedplants were transplanted into soil.
Abstract
An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA, 2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.