비타민 B1 유도체 Thiamine Dilauryl Sulfate의 나노 입자 제조를 통한 수용액의 용해도에 따른 항진균 활성 평가
Effect of Solubility of Thiamine Dilauryl Sulfate Solution through the Manufacture of the Nano Paticles on Antifungal Activity
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Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem,this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 ㎚ by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 ㎝ compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.
Abstract
Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem,this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 ㎚ by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 ㎝ compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.
참고문헌 (13)
- 안선미, "황련 추출물의 고추역병에 대한 In-vitro항진균 활성 및 고추 생육촉진 효과", 한국미생물·생명공학회지, 37(3), pp.280~286 (2009), 한국미생물·생명공학회
- Jo EK, "Plant pathogens and pest control pesticides", The Bimonthly Magazine for Agrochemicals and Plant Protection, 5, pp.33~41 (1984)
- Kim CS, "Development of biofungicide for control of gray mold rot of eggplant caused by Bortrytis cinerea and bioassay in the greenhouse condition", Korean Journal of Life Science, 11, pp.235~341 (2001)
- 김혁수, "티아민 디라우릴 황산염이 함유된 화장품의 항균활성 및 방부효과에 관한 연구", 오일 및 응용과학 학회지, 22(3), pp.212~218 (2005), 한국응용과학기술학회
- 김민희, "식물병원균에 광범위 항균활성을 가진 미생물의 분리 및 특성", KSBB Journal, 23(3), pp.219~225 (2008), 한국생물공학회
- 김성현, "아연 나노 입자와 산화아연 나노 입자의 특성과 식물독성", 대한환경공학회지, 31(12), pp.1129~1134 (2009), 대한환경공학회
- 고성욱, "알로에(Aloe arborescens M.)의 항진균성 검정을 통한 생물농약 가능성 탐색", 농업생명과학연구, 43(3), pp.35~44 (2009), 농업생명과학연구원
- Lee MJ, "SYNERGISTIC EFFECT OF VITAMIN B-1 ON SANITIZER AND DISINFECTANT TREATMENTS FOR REDUCTION OF BACILLUS CEREUS IN RICE", JOURNAL OF FOOD SAFETY, 30, pp.1~11 (2010), BLACKWELL PUBLISHING
- Lee YH, "Use of vitamin B1 as agents for controlling plant diseases. Korea Patent. 7,011,615", ~ (2002)
- 박창민, "3-O-Cetyl-L-Ascorbic Acid의 미백 개선 효과", 대한화장품학회지, 37(1), pp.91~96 (2011), 대한화장품학회
- Saxena A, "Effect of molecular weight heterogeneity on drug encapsulation effciency of gelatin nano-particles", Colloids and Surfaces B: Biointerfaces, 45, pp.42~48 (2005)
- 서삼열, "Xenorhabdus 및 Photorhabdus 세균 배양액을 이용한 생물농약 개발에 관한 연구", 한국응용곤충학회지, 49(3), pp.241~249 (2010), 한국응용곤충학회
- 서용창, "비타민 B1 유도체(Thiamine Di-lauryl Sulfate:TDS)의 나노입자화를 통한 고추탄저병균의 항진균 활성 증진", 한국약용작물학회지, 19(3), pp.198~204 (2011), 한국약용작물학회