ISSR 표지에 의한 천마의 유전 다양성분석 및 기능성 물질분석
Genetic Diversity and Metabolite Analysis of Gastrodia elata by Inter-Simple Sequence Repeats (ISSR) Markers
1
조회
0
다운로드
8
인용
0
참고문헌
키워드
초록
Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon’s information Index (S.I.)indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.
Abstract
Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon’s information Index (S.I.)indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.
참고문헌 (41)
- Camacho FJ, "Population structure and genetic diversity of Botrychium pumicola(Ophioglossaceae) based on inter-simple sequence repeats(ISSR)", American Journal of Botany, 88, pp.1065~1070 (2001)
- 최소라, "건조방법의 차이에 따른 천마의 품질 및 성분 변화", 한국약용작물학회지, 19(5), pp.354~361 (2011), 한국약용작물학회
- Cho DH, "A study on the genetic variation of Tricholoma matsutake collected from eleven sites of Korea using I-SSR PCR", Korean Journal of Mycology, 28, pp.32~37 (2000)
- Chung JM, "Fine-scale genetic structure among genetic individuals of the clone-forming monotypic genus Echinosophora koreensis(Fabaceae)", Annals of Botany, 98, pp.165~173 (2006)
- Cohen SA, "The pico-tag method: A manual of advanced techniques for amino acid analysis", Millipore Corp, 11~52 (1988)
- Excoffier L, "Analysis of molecular variance inferred from metric distance among DNA haplotypes: Application to human mitochondrial DNA restriction data", Genetics, 131, pp.479~491 (1992)
- Ge XJ, "Genetic diversity and geographic differentiation in endangered Ammopiptanthus(Leguminosae) populations in desert regions of northwest China as revealed by ISSR analysis", Annals of Botany, 95, pp.843~851 (2005)
- Hamrick JL, "Factors influencing levels of genetic diversity in woody plant species", New Forests, 6, pp.95~124 (1992)
- 홍용표, "남한지역 구상나무와 분비나무 집단에서의 nSSR 표지 유전 변이", 한국임학회지, 100(4), pp.577~584 (2011), 한국임학회
- Hong YP, "Diversity of I-SSR variants in the populations of Torreya nucifera", Journal of Korean Forest Society, 89, pp.167~172 (2000)
- 홍용표, "Distribution of I-SSR Variants in Natuak Populations of Smile Rosebay (Rhododendron schlippenbachii.) in korea", 한국임학회지, 92(5), pp.497~503 (2003), 한국임학회
- 홍용표, "설악산 격리 잔존 눈잣나무 집단의 유전적 성상", 한국임학회지, 93(5), pp.393~400 (2004), 한국임학회
- Hsieh CL, "Anticonvulsive and free radical scavenging activities of vanilyl alcohol in ferric chlorideinduced epileptic seizures in sprague-dawley rats", Life Sciences, 67, pp.1185~1195 (2000)
- Hsieh MT, "Gastrodine andhydroxybenzyl alcohol facilitate memory consolidation and retrieval, but not acquisition on the passive avoidance task in rats", Journal of Ethnopharmacology, 56, pp.45~54 (1997)
- Kim HJ, "Ether fraction of methanol extracts of Gastrodia elata, a traditional medicinal herb, protects against kainic acid-induced neuronal damage in the mouse hippocampus", Neuroscience Letters, 314, pp.65~68 (2001)
- Kim JS, "Genetic structure of natural populations of Pinus densiflora in Kangwon - Kyungbuk region", Korean Journal of Breeding, 24, pp.48~60 (1992)
- Kim JS, "Pinus densiflora for erecta - Can it be treated genetically as a distinct group? - Reconsideration based on allozyme data", Journal of Korean Forest Society, 82, pp.166~175 (1993)
- 김상용, "ISSR 자료에 기초한 만리화(물푸레나무과)의 유전적 다양성", 식물분류학회지, 39(1), pp.48~54 (2009), 한국식물분류학회
- Korpelainen H, "Mating system and distribution of enzyme genetic variation in bracken(Pteridium aquilinum)", Canadian Journal of Botany, 73, pp.1611~1617 (1995)
- 권해연, "I-SSR Variation Within and Among Korean Populations in Taxus cuspidata", 한국임학회지, 91(5), pp.654~660 (2002), 한국임학회
- Lee JY, "The propagation of Gastrodia elata by using rhizomorphs of Armillariella mella", Rural Development Research Service, 7, pp.39~45 (1982), Seoul Women’s University
- 이석우, "Genetic Variation of I-SSR Markers in the Natual Populations of a Rare and Endangered Tree Species, Oplopanax elatus in Korea", 한국임학회지, 91(5), pp.565~573 (2002), 한국임학회
- Lee WY, "Supplementation of methionine enhanced the ergothioneine accumulation in the Ganoderma neojaponicum Mycelia", Applied Biochemistry and Biotechnology, 158, pp.213~221 (2009)
- XiangQianLiu, "The Constituents of the Aerial Part of Gastrodia elata Blume", Natural Product Sciences, 8(4), pp.137~140 (2002), 한국생약학회
- Liu ZH, "Protective effects of gastrodine on the cellular model of Alzheimer’s disease induced by Abeta 25-35", Sichuan Da Xue Xue Bao, 36, pp.537~540 (2005)
- Michener CD, "A quantitative approach to a problem of classification", Evolution, 11, pp.490~499 (1957)
- Niu Q, "Effect of Gastrodia elata on learning and memory impairment induced by aluminum in rats", Journal of Hygiene Research, 33, pp.45~48 (2004)
- Noda N, "2, 4- Bis(4-hydroxybenzyl) phenol from Gastrodia elata", Phytochemistry, 39, pp.1247~1248 (1995)
- Nybom H, "Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Prospectives in Plant Ecology", Evolution and Systematics, 3, pp.99~114 (2000)
- Ong ES, "Determination of gastrodin and vanillyl alcohol in Gastrodia elata Blume by pressurized liquid extraction at room temperature", Journal of Separation Science, 30, pp.2130~2137 (2007)
- Page RDM, "TREEVIEW: An application to display phylogenetic trees on personal computers", Computer Applications in the Biosciences, 12, pp.357~358 (1996)
- Park EJ, "Ergothioneine accumulation in a medicinal plant Gastrodia elata", Journal of Medicinal Plants Research, 4, pp.1141~1147 (2010)
- 류재혁, "ISSR 표지에 의한 서양민들레와 흰민들레 수집종의 유전적 다양성 및 유연관계 분석", 한국약용작물학회지, 19(3), pp.149~156 (2011), 한국약용작물학회
- Shannon CE, "A mathematical theory of communication", Bell System Technical Journal, 27, pp.379~656 (1948)
- Son DS, "Genetic variation of isozymes in populations of Pinus densiflora and Pinus thunbergii naturally distributed in Kyungpook province", Journal of Korean Forest Society, 78, pp.345~359 (1989)
- Taguchi H, "Studies on the constituents of Gastrodia elata Blume", Chemical & Pharmaceutical Bulletin, 29, pp.55~62 (1981)
- Tao J, "Relationships among genetic makeup, active ingredient content, and place of origin of the medicinal plant Gastrodia tuber", Biochemical Genetics, 47, pp.8~18 (2009)
- Wright S, "Evolution and the genetics of population(Vol. 4). Variability within and among natural populations" (1978), University of Chicago Press
- Xiao LQ, "ISSR variation in the endemic and endangered plant Cycas guizhouensis(Cycadaceae)", Annals of Botany, 94, pp.133~138 (2004)
- Xiao M, "ISSR analysis of the genetic diversity of the endangeredspecies Sinopodophyllum hexandrum(Royle) Ying from westernSichuan province, China", Journal of Integrative Plant Biology, 48, pp.1140~1146 (2006)
- 양병훈, "ISSR 표지에 의한 우리나라 야생 차나무집단의 유전다양성", 한국차학회지, 16(3), pp.119~126 (2010), 한국차학회