발광플라즈마 처리에 의한 들깨 부위별 항산화 및 Tyrosinase 저해 활성 효과
Antioxidant and Tyrosinase Inhibition Activity Promoting Effects of Perilla by the Light Emitting Plasma
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Thelightemittingplasma(LEP)hasrecentlyattractedattentionasanovelartificiallightsourceforplantgrowthandfunc- tionalcomponentenhancement.WeinvestigatedtheeffectsofLEPonwhiteningandantioxidantactivitiesoftheplantpartsofperilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonicacid (ABTS),reducingpowerandtyrosinaseinhibitionactivity asindicatorsofbiologicalactivity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total fla- vonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power val- ues of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of bio- logical activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.
Abstract
Thelightemittingplasma(LEP)hasrecentlyattractedattentionasanovelartificiallightsourceforplantgrowthandfunc- tionalcomponentenhancement.WeinvestigatedtheeffectsofLEPonwhiteningandantioxidantactivitiesoftheplantpartsofperilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonicacid (ABTS),reducingpowerandtyrosinaseinhibitionactivity asindicatorsofbiologicalactivity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total fla- vonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power val- ues of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of bio- logical activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.
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