과도 운동 후 면역능 개선에 미치는 황기 다당체의 효과
Effect of Astragalus membranaceus Polysaccharides on Improves Immune Response after Exhaustive Exercise Rats
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Background: Polysaccharides are the most important functional constituent in Astragalus membranaceus. The purpose of the present study was to evaluate the effect of polysaccharides isolated from the aboveground parts of A. membranaceus (AMA) and polysaccharides isolated from the roots of A. membranaceus (AMR) immune function by modulated cytotoxic T cell and Th1- and Th2- related cytokines kinetics. Methods and Results: Sprague-Dawley rats were randomly divided into exhaustive exercise case groups and non-exercise case, AMA and AMR samples were administered orally for 30 days (500 ㎎/㎏/day and 10 ㎎/㎏/day, respectively) and were compared to those rats in the groups fed commercial sports drink (SPD) and vehicle. Both exhaustive exercise groups and non-exercise groups had a lower ratio of CD4+ and CD8+ cells in the spleens of the rat fed AMA and AMR compared to those in the rats fed SPD and vehicle group. These results suggested that AMA and AMR promote an increase in the proportion of cytotoxic T cells. The IL-4- producing T lymphocytes decreased significantly in the AMR (10 ㎎/㎏/day) group compared to SPD and vehicle, whereas the AMA group increased the IL-4 concentration more than the SPD and vehicle in exhaustive exercise group. However, the populations of IFN-γ-producing T lymphocytes of AMR and AMA increased. AMA decreased the concentration of IFN-γ to inhibit the Th1 response and thereby increased the concentration of IL-4 to induce a Th2 response that was related to humoral immunity in the non-exercise group. Conclusions: These results showed that, in addition to Th1/Th2 regulation, AMR and AMA played an important immuno-modulatory role after exhaustive exercise-induced Th1/Th2 lymphocyte imbalance, which might be correlated with cytokine producing immunoregulatory cells.
Abstract
Background: Polysaccharides are the most important functional constituent in Astragalus membranaceus. The purpose of the present study was to evaluate the effect of polysaccharides isolated from the aboveground parts of A. membranaceus (AMA) and polysaccharides isolated from the roots of A. membranaceus (AMR) immune function by modulated cytotoxic T cell and Th1- and Th2- related cytokines kinetics. Methods and Results: Sprague-Dawley rats were randomly divided into exhaustive exercise case groups and non-exercise case, AMA and AMR samples were administered orally for 30 days (500 ㎎/㎏/day and 10 ㎎/㎏/day, respectively) and were compared to those rats in the groups fed commercial sports drink (SPD) and vehicle. Both exhaustive exercise groups and non-exercise groups had a lower ratio of CD4+ and CD8+ cells in the spleens of the rat fed AMA and AMR compared to those in the rats fed SPD and vehicle group. These results suggested that AMA and AMR promote an increase in the proportion of cytotoxic T cells. The IL-4- producing T lymphocytes decreased significantly in the AMR (10 ㎎/㎏/day) group compared to SPD and vehicle, whereas the AMA group increased the IL-4 concentration more than the SPD and vehicle in exhaustive exercise group. However, the populations of IFN-γ-producing T lymphocytes of AMR and AMA increased. AMA decreased the concentration of IFN-γ to inhibit the Th1 response and thereby increased the concentration of IL-4 to induce a Th2 response that was related to humoral immunity in the non-exercise group. Conclusions: These results showed that, in addition to Th1/Th2 regulation, AMR and AMA played an important immuno-modulatory role after exhaustive exercise-induced Th1/Th2 lymphocyte imbalance, which might be correlated with cytokine producing immunoregulatory cells.
참고문헌 (50)
- Abbas AK, "Cellular and molecular immunology, In immunity to microbes", pp.2012, Elsevier
- Brooks GA, "Determination of metabolic and heart rate responses of rats to treadmill exercise", Journal of Applied Physiology, 45, pp.1009~1015 (1978)
- Cho WCS, "In vitro and in vivo antitumor effects of Astragalus membranaceus", Cancer Letters, 252, pp.43~54 (2007)
- Cho WCS, "In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus", Journal of Ethnopharmacology, 113, pp.132~141 (2007)
- 최리나, "황기 지상부로부터 장관면역 활성 다당체의 분리 및 단회 경구 투여 독성시험", 한국약용작물학회지, 22(4), pp.276~288 (2014), 한국약용작물학회[DOI]
- Chu DT, "Immunotherapy with Chinese medicinal herbs. Part II. Reversal of cyclophosphamideinduced immune suppression by administration of fractionated Astragalus membranaceus in vivo", Journal of Clinical and Laboratory Immunology, 25, pp.125~129 (1988)
- Cowles WN, "Fatigue as a contributory cause of pneumonia", Boston Medical and Surgical Journal, 179, pp.555~556 (1918)
- Dinarello CA, "Interleukin-1, interleukin-1 receptors and interleukin-1 receptor antagonist", International Reviews of Immunology, 16, pp.457~499 (1998)
- Dion C, "Does larch arabinogalactan enhance immune function? A review of mechanistic and clinical trials", Nutrition and Metabolism, 13, pp.28~ (2016)
- dos Santos RV, "Effect of exercise on glutamine metabolism in macrophages of trained rats", European Journal of Applied Physiology, 107, pp.309~315 (2009)
- Gholamnezhad Z, "Evaluation of immune response after moderate and overtraining exercise in wistar rat", Iranian Journal of Basic Medical Sciences, 17, pp.1~8 (2014)
- Gu X, "The effect of influenza virus A on th1", th2 balance and alveolar fluid clearance in pregnant rats, 2011, pp.37, 445~451
- Hackney AC, "The immune system and overtraining in athletes: Clinical implications", Acta Clinica Croatica, 51, pp.633~641 (2012)
- Hao S, "Effects on exercise endurance capacity and antioxidant properties of Astragalus membranaceus polysaccharides(APS)", Journal of Medicinal Plants Research, 4, pp.982~986 (2010)
- Jeurissen A, "The effects of physical exercise on the immune system", Nederlands Tijdschrift Voor Geneeskunde, 147, pp.1347~1351 (2003)
- Ji LL, "Myocardial aging: Antioxidant enzyme systems and related biochemical properties", American Journal of Physiology, 261, pp.386~392 (1991)
- Jin R, "Effects of shi-ka-ron and Chinese herbs in mice treated with anti-tumor agent mitomycin C", Chinese Journal of Integrated Traditional and Western Medicine, 15, pp.101~103 (1995)
- Kaiko GE, "Immunological decision-making: How does the immune system decide to mount a helper T-cell response?", Immunology, 123, pp.326~338 (2008)
- Kilciler G, "Do the changes in the serum levels of IL-2, IL-4, <TEX>$TNF{\alpha}$<", TEX>, and IL-6 reflect the inflammatory activity in the patients with post-ERCP pancreatitis?, 2008, pp.2008, 481560~
- 김철우, "운동빈도의 차이가 식이유도 운동알레르기 질환과 관련기전에 미치는 영향", 생명과학회지, 22(7), pp.897~903 (2012), 한국생명과학회[DOI]
- 김철우, "Combined effects of food and exercise on anaphylaxis", Nutrition Research and Practice, 7(5), pp.347~351 (2013), 한국영양학회
- Kwon DK, "Effect of swimming exercise and soybean supplementation on the immune functions of rats fed a high-fat diet", Clinical and Experimental Pharmacology and Physiology, 35, pp.638~642 (2008)
- Lakier SL, "Overtraining, excessive exercise, and altered immunity: Is this a T helper-1 versus T helper-2 lymphocyte response?", Sports Medicine, 33, pp.347~364 (2003)
- Lancaster GI, "Effects of acute exhaustive exercise and chronic exercise training on type 1 and type 2 T lymphocytes", Exercise Immunology Review, 10, pp.91~106 (2004)
- Lee KY, "Macrophage activation by polysaccharide isolated from Astragalus membranaceus", International Immunopharmacology, 5, pp.1225~1233 (2005)
- Lim JD, "Structural characterization of an intestinal immune system-modulating arabino-3,6-galactan-like polysaccharide from the aboveground part of Astragalus membranaceus(Bunge)", Carbohydrate Polymers, 136, pp.1265~1272 (2016)
- Mackinon LT, "Mucosal(secretory) immune system responses to exercise of varying intensity and during overtraining", International Journal of Sports Medicine, 15, pp.S179~S183 (1994)
- McAlees JW, "Epigenetic regulation of beta2-adrenergic receptor expression in TH1 and TH2 cells", Brain, Behavior, and Immunity, 25, pp.408~415 (2011)
- McElhaney JE, "A placebocontrolled trial of a proprietary extract of North American ginseng(CVTE002) to prevent acute respiratory illness in institutionalized older adults", Journal of the American Geriatrics Society, 52, pp.13~19 (2004)
- Mosmann TR, "Two types of murine helper T cell clone. Part I. Definition according to profiles of lymphokine activities and secreted proteins", Journal of Immunology, 136, pp.2348~2357 (1986)
- Nieman DC, "Marathon training and immune function", Sports Medicine, 37, pp.412~415 (2007)
- Penkowa M, "Immunohistochemical detection of IL-6 in human skeletal muscle fibers following exercise", FASEB Journal, 17, pp.2166~2168 (2003)
- Predy GN, "Efficacy of an extract of North American ginseng containing poly-furanosyl-pyranosyl-saccharides for preventing upper respiratory tract infections: A randomized controlled trial", Canadian Medical Association Journal, 173, pp.1043~1048 (2005)
- Rihoux JP, "Understanding allergy", Allergy Immunology, 10, pp.5~17 (1988)
- Rittenhouse JR, "Chinese medicinal herbs reverse macrophage suppression induced by urological tumors", Journal of Urology, 146, pp.486~490 (1991)
- Rohde T, "The immune system and serum glutamine during a triathlon", European Journal of Applied Physiology and Occupational Physiology, 74, pp.428~434 (1996)
- Rosa LFBPC, "Exercise as a time-conditioning effector in chronic Disease: A complementary treatment strategy", Evidence Bed Complementary and Alternative Medicine, 1, pp.63~70 (2004)
- Rosa Neto JC, "Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats", European Journal of Applied Physiology, 106, pp.697~704 (2009)
- Schepetkin IA, "Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential", International Immunopharmacology, 6, pp.317~323 (2006)
- Schroder WA, "SerpinB2 deficiency modulates Th1", Th2 responses after schistosome infection, 2010, pp.32, 764~768
- Shimizu J, "Proportion of murine cytotoxic T cells is increased by high molecular-weight fucoidan extracted form Okinawa mozuku(Cladosiphon okamuranus)", Journal of Health Science, 51, pp.394~397 (2005)
- Sun CW, "Effects of Astragalus saponins on <TEX>$Ca^{2+}$<", TEX>-activated K+ channels of mouse T lymphocytes, 1996, pp.2, 125~126
- Udani JK, "Immunomodulatory effects of <TEX>$ResistAid^{TM}$<", TEX>: A randomized, double-blind, placebo-controlled, multidose study, 2013, pp.32, 331~338
- Verde T, "Potential markers of heavy training in highly trained distance runners", British Journal of Sports Medicine, 26, pp.167~173 (1992)
- Walsh NP, "Position statement part one: Immune function and exercise", Exercise Immunology Review, 17, pp.6~63 (2011)
- Wei H, "Traditional Chinese medicine Astragalus reverses predominance of Th2 cytokines and their up-stream transcript factors in lung cancer patients", Oncology Reports, 10, pp.1507~1512 (2003)
- Yang P, "The mutations of Th1 cell-specific T-box transcription factor may be associated with a predominant Th2 phenotype in gastric cancers", International Journal of Immunogenetics, 37, pp.111~115 (2010)
- 양수진, "황기 지상부 다당체의 면역 및 백신보조 효과", 한국약용작물학회지, 24(5), pp.408~419 (2016), 한국약용작물학회[DOI]
- Yoshida Y, "Immunomodulating activity of Chinese medicinal herbs and Oldenlandia diffusa in particular", International Journal of Immunopharmacology, 19, pp.359~370 (1997)
- Zhao Y, "Altered expressions of helper T cell(Th)1, Th2, and Th17 cytokines in <TEX>$CD8^{+}$<", TEX> and <TEX>${\gamma}{\delta}$<, Journal of Asthma, pp.2011, 48[DOI]