Phytophthora sansomeana에 의한 큰꽃삽주 역병 발생 보고
Occurrence of the Phytophthora Blight Caused by Phytophthora sansomeana in Atractylodes macrocephala Koidz.
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.
Abstract
Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.
참고문헌 (23)
- Chang KF, "First report of Phytophthora sansomeana causing root rot in field pea in Alberta, Canada", Crop Protection, 101, pp.1~4 (2017)
- Cho JH, "Genetic variation of Atractylodes macrocephala in morphological traits and Phytophthora root rot resistance", Korean Journal of Breeding Science, 33, pp.191~198 (2001)
- 조준형, "포장조건에 따른 백출의 역병 발생", 한국약용작물학회지, 9(3), pp.211~219 (2001), 한국약용작물학회
- Cooke DEL, "A molecular phylogeny of Phytophthora and related oomycetes", Fungal Genetics and Biology, 30, pp.17~32 (2000)
- Hansen EM, "Phytophthora rosacearum and P. sansomeana, new species segregated from the Phytophthora megasperma “complex”", Mycologia, 101, pp.129~135 (2009)
- 정현수, "삽주 육성품종 뿌리의 항산화 및 항염증 효과 비교", 한국약용작물학회지, 26(5), pp.391~400 (2018), 한국약용작물학회[DOI]
- 정진태, "삽주 육성품종 간 생육특성 및 유효성분 분석", 한국약용작물학회지, 26(3), pp.220~226 (2018), 한국약용작물학회[DOI]
- Jerng U., "Comparative study on efficacy of Atractylodes rhizome recorded in traditional medical literature and in recent pharmacological article", Korean Herbal Medicine Informatics, 3, pp.1~16 (2015)
- Katoh K, "MAFFT multiple sequence alignment software version 7: Improvements in performance and usability", Molecular Biology and Evolution, 30, pp.772~780 (2013)
- Kim HK, "Occurrence of rhizome rot of Atractylodes spp. caused by Phytophthora drechsleri", Korean Journal of Plant Pathology, 13, pp.433~437 (1997)
- Kimura M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", Journal of Molecular Evolution, 16, pp.111~120 (1980)
- Ko WH, "A microsyringe method for determining concentration of fungal propagules", Phytopathology, 63, pp.1206~1207 (1973)
- Lee JH, "Studies of taxonomic origins of Atractylodis rhizoma Alba and Atractylodis rhizoma", Korean Journal of Oriental Medicine, 8, pp.55~63 (2002)
- Production results of special crops, "Ministry of Agriculture, Food and Rural Affairs", Ministry of Agriculture, Food and Rural Affairs, 41~60 (2019)
- The Korea pharmacopoeia, "Ministry of Food and Drug Safety", Ministry of Food and Drug Safety, 773~774 (2000)
- The Korea pharmacopoeia, "Ministry of Food and Drug Safety", Ministry of Food and Drug Safety, 44~ (2019)
- Compendium of medicinal plant diseases with colour plates, "National Institute of Agricultural Science(NIAS)", National Institute of Agricultural Science and Technology, 68~70 (1991)
- Phytophthora diseases in Korea, "National Institute of Agricultural Science(NIAS)", National Institute of Agricultural Science, 41~45 (2000)
- Tamura K, "MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Molecular Biology and Evolution, 28, pp.2731~2739 (2011)
- Tang QH, "First report of root rot caused by Phytophthora sansomeana on soybean in China", Plant Disease, 94, pp.378~378 (2010)
- White TJ, "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics", pp.315~322 (1990), Academic Press
- Guidelines for ATC classification and DDD assignment 2015, "World Health Organization(WHO)", World Health Organization, 275~ (2014)
- Zelaya-Molina LX, "First report of Phytophthora sansomeana causing wilting and stunting on corn in Ohio", Plant Disease, 94, pp.125~125 (2010)