사철쑥 추출물의 면역세포의 생육증진 및 세포독성

Abstract

The immune activation and anticancer activities of the water and ethanol extracts from Artemisia capillaris Thunb. were studied. The growth of human hepatocarcinoma and human gastric cancer cell was inhibited by the addition of 1.0㎎/㎖ of the water extract, by about 77% and 95%, respectively. The growth of human breast cancer cells was also inhibited by addition of 0.5㎎/㎖ of both water and ethanol extracts by 88%. The growth of human normal lung cell, HEL299 was inhibited by 15% indicating very low cytotoxicity of both extracts. Overall selectivity of the both extracts on several human cancer cell line was over 2.5. The growth of both human B and T cells was enhanced up to 1.6 to 2.1 times by adding the ethanol extracts. The secretion of cytokines, TNF-α and IL-6, from human B cells was also increased showing 68pg/㎖ and 67pg/㎖ respectively, compared to 35~40pg/㎖ of the control. In terms of the immune activity, there was not much difference between water and ethanol extracts of Artemisia capillaris Thunb. It implies that the extraction solvent could not differ the biological activities of the extracts. Based on these results, Artemisia capillaris Thunb. can be developed into a potentially useful cancer chemoprentive agent.