Optimal Culture Conditions for in vitro Propagation of Orostachys japonicus and Enhancement of Polysaccharide Production

Abstract

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of ploysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micro propagated shoots were successfully acclimatized artificial soils.